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Rhodiola sachalinensis extract and application thereof

A technology of Rhodiola rosea, extract, applied in application, biocide, animal repellant, etc.

Active Publication Date: 2021-03-26
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the inhibition of ginseng pathogenic bacteria by plant-derived pesticide Rhodiola rosea extract

Method used

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  • Rhodiola sachalinensis extract and application thereof
  • Rhodiola sachalinensis extract and application thereof
  • Rhodiola sachalinensis extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 Test material

[0031] Test material: dry roots of Rhodiola rosea. Origin: Baishan City, Jilin Province, purchased from Baishan Lanxin Specialty Products Trading Co., Ltd.

[0032] Standard products and reagents for testing: salidroside (standard product), tyrosol (standard product), rosevi (standard product), kaempferol (standard product). All four standard products were purchased from Baoji Chenguang Biotechnology Co., Ltd.

[0033] Test agent: 50% carbendazim wettable powder (Jiangsu Lanfeng Biochemical Co., Ltd.), 30% Jingjia·hymexazol aqueous solution (Zhejiang Heben Technology Co., Ltd.), 2.5% fludioxonil suspension Coating agent (Syngenta Nantong Crop Protection Co., Ltd.).

[0034] Bacteria tested: Phytophthora blight of ginseng ( Rhizoctonia solani ), ginseng damping-off bacteria ( Python debaryanum ), ginseng root rot ( F. oxysporum) , Panax ginseng ( Alternaria panax ), Botrytis ginseng ( Botrytis cinerea ), Sclerotinia ginseng ( ...

Embodiment 2

[0039] Example 2 Preparation of Rhodiola rosea water extract

[0040] Weigh 20g of the dried root of Rhodiola rosea, put it in an appropriate container, add 100 times the amount of distilled water to soak, and soak for 5d. After soaking, filter with non-woven cloth, collect filtrate and filter residue respectively. Put the filter residue in a stainless steel pot, add 30 times the amount of distilled water into the pot and cook for 20 minutes. After decocting, use non-woven cloth to filter and collect the Rhodiola rosea filtrate. Mix the filtrate soaked in distilled water and the filtrate decocted, use a rotary evaporator to concentrate, and stop the operation when the volume of the concentrated solution reaches about 10mL. The water extract of Rhodiola rosea was obtained by freeze-drying, sealed and stored in a brown reagent bottle, and placed in a refrigerator at 4°C until use.

Embodiment 3

[0041] Example 3 Preparation of fine Rhodiola rosea extract

[0042]Take a certain volume of macroporous resin (D101) in a large beaker, slowly add ethanol solution with a volume concentration of 95% to the beaker until the liquid level of the ethanol solution exceeds the macroporous resin particles, let it stand still for about 12 hours, rinse with distilled water until it is odorless After loading the column, fill it to two-thirds of the glass column, configure the water extract of Rhodiola rosea to a concentration of 5 mg / mL for loading, and the loading volume is one-third of the volume of the macroporous resin. After loading, stand still for 12 Hours, use distilled water, 10%, 20%, 30%...80% ethanol in turn to wash, collect different eluted components, concentrate the eluent, and dry it into powder. Number a: water eluate, b : 10% ethanol eluate, c: 20% ethanol eluate, d: 30% ethanol eluate...i: 80% ethanol eluate.

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Abstract

The invention discloses a rhodiola sachalinensis water extract prepared by the steps: drying roots of rhodiola sachalinensis, adding water for soaking, filtering, collecting filtrate and filter residues, adding water into the filter residues for decocting, filtering after decocting, collecting filtrate, mixing the two filtrates, concentrating, and freeze-drying to obtain the rhodiola sachalinensisaqueous extract; and carrying out macroporous resin dynamic adsorption-elution, eluting with water, discarding the eluent, eluting with a 10% ethanol solution, collecting the eluent, evaporating to remove the solvent, and thus obtaining the rhodiola crenulata extract. When the concentration of the rhodiola sachalinensis aqueous extract is 2 mg / mL, the inhibition rate of the rhodiola sachalinensisaqueous extract on ginseng rhizoctonia solani kuhn can reach 100%, and when the concentration of the rhodiola sachalinensis aqueous extract is 3 mg / mL, the inhibition rate of the rhodiola sachalinensis aqueous extract on ginsengrhizoctonia solani kuhn is 50%. When the refined rhodiola sachalinensis extract is 0.5 mg / mL, the inhibition rate of the rhodiola sachalinensis extract on ginseng rhizoctonia solani kuhn is 47.47%, and the inhibition rate of the rhodiola sachalinensis extract on ginseng rhizoctonia solani kuhn reaches 97.47%.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and specifically relates to the extract of Rhodiola rosea alpine and its application. Background technique [0002] Rhodiola alpine ( Rhodiolasachalinensis ), also known as Sakhalin Rhodiola, is a wild plant that grows in the tundra zone with harsh environment and changeable climate at an altitude of 1700-2500 meters, with strong vitality. Rhodiola rosea plays a more significant role in resistance tests such as anti-hypoxia, anti-radiation, anti-fatigue, and anti-virus. Alpine Rhodiola is mainly produced in Changbai Mountains of Jilin Province, Shangzhi, Hailin, Ning'an and other counties of Heilongjiang Province. Among them, Changbai Mountains of Jilin Province took the lead in planting Rhodiola in the 1980s, and the development area is relatively large. "The Hometown of Sedum" has been planted on a large scale in the mountainous areas of the three northeastern provinces. T...

Claims

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Application Information

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IPC IPC(8): A01N65/08A01N25/04A01P3/00A01P1/00
CPCA01N65/08A01N25/04
Inventor 马贵龙高新馨赵成爱袁守超张婷婷
Owner JILIN AGRICULTURAL UNIV
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