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Method for calibrating ICP-MS trace element analyzer by using internal standard combined solution

A technology of trace elements and internal standard elements, which is applied in the analysis of materials, material analysis by electromagnetic means, instruments, etc., can solve the problems affecting the detection of trace elements in blood, too many internal standard combinations, and reduced sensitivity. The effect of human error, simple operation of preparation work and high sensitivity

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Problems solved by technology

However, when using the same internal standard system to detect multiple trace elements, it is necessary to use various elements with similar mass numbers and ionization energies as the internal standard, resulting in too many currently available internal standard combinations, resulting in the use of inconvenient
However, if only too few internal standard quantities (such as 1-2 types) are used, although the cost can be reduced and the use is convenient, there is a problem of reduced sensitivity due to the lack of necessary internal standard elements with similar mass numbers of the elements to be measured. Insufficient, affecting the application of ICP-MS detection of trace elements in blood

Method used

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  • Method for calibrating ICP-MS trace element analyzer by using internal standard combined solution
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  • Method for calibrating ICP-MS trace element analyzer by using internal standard combined solution

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Embodiment 1

[0080] Embodiment 1: Preparation of internal standard calibrator of manganese element in human whole blood

[0081] (1) 1% HNO 3 : Measure 5mL of HNO 3 , dilute to a 500mL volumetric flask with purified water, and mix well;

[0082] (2) Preparation of sample diluent: measure 0.5mL of HNO 3 , 0.5mL of Triton, 10mL of isopropanol were distilled to a 500mL volumetric flask with purified water, and mixed;

[0083] (3) Manganese internal standard calibration product preparation in human whole blood: the single element standard solution of germanium Ge and scandium Sc is diluted 100 times with 1% HNO3, the total volume is 10mL, and the concentration is 10mg / L to obtain the manganese element in human whole blood Internal standard stock solution; then take 0.4mL human whole blood manganese element internal standard stock solution and dilute it 100 times with sample diluent, the total volume is 100mL, the concentration is 40μg / L, and the human whole blood manganese element internal ...

Embodiment 2

[0084] Embodiment 2: Calibrate the trace element analyzer with internal standard calibrator

[0085] The purpose of the experiment: to calibrate and optimize the repeatability and stability of the trace element analyzer (Clin-ICP-QMS-I) by using the internal standard calibrator.

[0086] experimental method:

[0087] (1) Put the internal standard calibration product at room temperature (10-30°C), shake well and set aside.

[0088] (2) When testing, inject the internal standard calibrator and external standard calibrator (or quality control product, or unknown sample) at the same time.

[0089] (3) Adjust the parameters of the trace element analyzer (Clin-ICP-QMS-I) to the best state according to the instrument operating procedures. After the instrument is stabilized, the internal standard calibrator and the external standard calibrator are injected at the same time, and the external standard calibrator is injected sequentially (from No. 0 to No. 5). Each sample needs to cons...

Embodiment 3

[0091] Example three: detection of manganese element content in 116 cases of human whole blood samples

[0092] experimental method:

[0093] 1. Sample collection and storage

[0094] (1) The blood collection personnel should try not to wear latex gloves when collecting blood. They can use plastic gloves, or clean their hands without gloves; Wipe the iodine with alcohol); (2) Collect about 2mL of whole blood using a sodium heparin anticoagulant blood collection tube, make sure the cap is tightly closed, and immediately mix by inverting gently to avoid coagulation. If it cannot be submitted for inspection within 2 hours, it should be stored at 2-8°C for a maximum of 14 days.

[0095] 2. Sample pretreatment

[0096] Dilute 20 times: Whole blood sample (>200μL) is mixed with a blood mixer for 5-8 minutes, pipette 100μL blood sample into a centrifuge tube containing 1.9mL diluent with a calibrated pipette gun, and place in a vortex Mix well on the shaker and wait for testing o...

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Abstract

The invention discloses a method for calibrating an ICP-MS trace element analyzer through an internal standard combined solution. The method comprises the following steps: preparing the internal standard combined solution; sampling the internal standard calibration product and the external standard calibration product at the same time; adjusting parameters of a trace element analyzer; sampling theinternal standard calibration product and the external standard calibration product at the same time; drawing a calibrated standard working curve; and detecting signals of trace elements and internalstandard elements in the sample to be detected, calculating the ratio of the trace elements to the internal standard elements, and calculating the concentration of the trace elements to be detected on the basis of the calibrated standard working curve. According to the method, the preparation work before computer detection is simple and rapid to operate, the probability of manual operation errorscaused by tedious preparation is reduced, and when the standard working curve is directly adopted for testing, an internal standard method is used for correction, so that the optimal test result canbe obtained.

Description

technical field [0001] The invention belongs to the field of biological detection, and relates to an internal standard calibrator for measuring Mn element in human whole blood by using inductively coupled plasma mass spectrometry, and a method for measuring Mn element in human whole blood by using inductively coupled plasma mass spectrometry. Background technique [0002] According to the content of elements in the human body, the elements in the human body can be divided into macro elements and trace elements. The macro elements refer to the elements that account for more than one ten thousandth of the body weight, including calcium (Ca), sodium (Na), phosphorus (P), Potassium (K), Magnesium (Mg), Chlorine (Cl) and Sulfur (S), etc., while trace elements refer to content below one ten-thousandth of the total amount of the human body, and the daily intake is low Elements in 100mg. In 1995, copper (Cu), cobalt (Co), chromium (Cr), iron (Fe), fluorine (F), iodine (I), manganes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/626
CPCG01N27/626
Inventor 马庆伟朱柯文熊婵向华
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