Method for determining content of AEEA-AEEA

Abstract

The invention discloses a method for determining the content of AEEA-AEEA. The method comprises the following steps: diluting a to-be-detected sample, and detecting the diluted sample in a high performance liquid chromatograph; the chromatographic conditions are as follows: a chromatographic column is Welch Ultra HILIC Silica, 250mm * 4.6 mm < 5 > [mu] m; the mobile phase flow velocity is 1.0 mL / min; the column temperature is 35 + / -2 DEG C; the detection wavelength is 205 nm; the gradient elution program is as following within 0-10 min, the mobile phase A is increased from 10% to 20%, the mobile phase B is decreased from 90% to 80%, within 10-45 min, the mobile phase A is increased from 20% to 30%, the mobile phase B is decreased from 80% to 70%, within 45-50 min, the mobile phase A is kept at 30%, the mobile phase B is kept at 70%, within 50-51 min, a the mobile phase A is decreased from 30% to 10%, the mobile phase B is increased from 70% to 90%, within 51-66 min, the mobile phase Ais maintained at 10%, and the mobile phase B is maintained at 90%. wherein the mobile phase A is a 0.01 mol / L ammonium acetate aqueous solution, and the mobile phase B is acetonitrile. According to the method, the product can be effectively separated from each impurity, so that the content detection of the target product is realized.

Description

technical field [0001] The invention belongs to the field of chemical analysis, in particular to a method for measuring AEEA-AEEA content. Background technique [0002] AEEA-AEEA, the Chinese name is 17-amino-10-oxo-3,6,12,15-tetraoxa-9-azaheptadecanoic acid, in the process of producing AEEA-AEEA, various Impurities, among which 2A methyl ester, AEEA, β-Ala-AEEA (β-Ala-A), β-Ala-2A and 3A are the main impurities. The polarity of the product AEEA-AEEA and the impurities is relatively large, and there is no determination method for AEEA-AEEA and the above-mentioned impurities in the prior art to detect the content of AEEA-AEEA. Contents of the invention [0003] In order to solve the above problems, the present invention provides a method for determining the content of AEEA-AEEA, which is based on high-performance liquid chromatography and can effectively separate the target product from other impurities, thereby accurately determining the content of the target product. ...

AI Technical Summary

Problems solved by technology

The polarity of the product AEEA-AEEA and each impurity is relatively large, and there is no AEEA-AEEA and the determination method of the above-mentioned impurities in the prior art to detect the content of AEEA-AEEA

Images