A method for detecting amantadine content

Abstract

The invention discloses a method for rapidly detecting the content of amantadine. The method includes the steps that a, 100 microliters of a cucurbit[7]uril solution are placed into a micro tube, 100 microliters of a sample solution containing amantadine is added into the micro tube for a reaction at the temperature of 20-30 DEG C, reaction time is 0.5-1 hour, and a cucurbit[7]uril @amantadine complex solution is obtained after the reaction, wherein the amount-of-substance concentration of the cucurbit[7]uril solution is 0.25-0.6 mM; b, 25 microliters of an ABTS solution and 25 microliters of a hydrogen dioxide solution are added into the obtained cucurbit[7]uril@amantadine complex solution, the mixture is heated to 40-50 DEG C, reaction time is 0.5-1 hour, and a detection solution is obtained after a color development reaction, wherein the amount-of-substance concentration of the ABTS solution is 30-50 mM, and the amount-of-substance concentration of the hydrogen dioxide solution is 25-50 mM; c, an ultraviolet-visible spectrophotometer is adopted for measuring the ultraviolet-visible absorption spectrum of the detection solution obtained after the color development reaction, and the content of the amantadine is calculated according to the relation between an absorbance value at the position of 420 nm wave length and the concentration of the amantadine. The method is easy, convenient and rapid to operate, and makes sensitivity high and specificity good.

Description

technical field [0001] The invention relates to a method for quickly detecting the content of amantadine, which belongs to the technical field of analytical chemistry. Background technique [0002] Amantadine, also known as tricyclodecanylamine, is a common drug molecule with a symmetrical structure. Pharmacological studies have shown that amantadine can prevent the virus from penetrating into host cells and effectively inhibit the uncoating and reproduction of the virus; at the same time, it can promote the release of dopamine transmitters from dopaminergic neurons and inhibit the reuptake of dopamine in brain tissue. Therefore, amantadine has important applications in antiviral and treatment of Parkinson's syndrome. However, overdose of amantadine can cause adverse reactions such as drowsiness, dizziness, auditory hallucinations, and nervous system damage. In order to avoid the excessive intake of amantadine as much as possible, it is particularly important to use an acc...

AI Technical Summary

Problems solved by technology

Although these methods have their own advantages in terms of detection sensitivity, specificity, and stability, the commonly used amantadine detection methods generally have disadvantages such as long detection time, expensive equipment, and cumbersome operations.

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