Plectasin secretory probiotic agent and preparation method and application thereof

A plectasiamycin, secretion-type technology, applied in the field of pphaceliamycin-secretion probiotics and its preparation, can solve the problems that the antibacterial activity needs to be further improved, so as to improve the quality of animal products, reduce drug resistance, and reduce disease rate effect

Active Publication Date: 2021-03-30
广州市微生物研究所集团股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The strain has both the dual functions of plectasin antibacterial peptide and Clostridium tyrobutyricum. Although it has a certain inhibitory effect on bacteria, its antibacterial activity and the effect of improving immunity still need to be further improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation of embodiment 1 plectasomycin secretion type probiotics

[0036] 1. Preparation of Plectasin-secreting Clostridium tyrobutyricum Powder

[0037] (1) According to the existing technology "Jia Junhao, Cao Ding, Chen Mianhua, et al. Study on the expression of mycelia in Clostridium tyrobutyricum [J]. Food Industry Science and Technology, 2020 (18). http: / / kns. cnki.net / kcms / detail / 11.1759.TS.20200408.1414.017.html"Preparation of plectasin-secreting Clostridium tyrobutyricum strain.

[0038] (2) After expanding the culture, prepare 30L fermentation medium in a 50L fermenter, inoculate the mycelia secreting Clostridium tyrobutyricum strain with an inoculum size of 8%, control the pH value of the fermentation process to be 6.5, and the growth temperature to be 37°C , the whole process is strictly anaerobic, the liquid volume is 60%, and 15% glucose is used to feed intermittently in the middle and late stages of fermentation. Glucose with a mass concentration ...

Embodiment 2

[0043] The preparation of embodiment 2 plectasomycin secretion type probiotics

[0044] Weigh each component in the following mass percentages: 35% of Plectasin-secreting Clostridium tyrobutyricum powder, 30% of Lactobacillus plantarum powder, 20% of Lactobacillus reuteri powder, 5% of fructooligosaccharides, and 10% of lactose . Among them, the number of live bacteria of the plectasin-secreting Clostridium tyrobutyricum powder was 3×10 8 cfu / g, the protective agent formula is according to the mass ratio Fumei powder: rice bran: corn starch = 75:10:15; the number of live bacteria of Lactobacillus plantarum powder is 5×10 11 cfu / g, the protective agent formula is skimmed milk powder in mass ratio: trehalose: sodium glutamate=30:5:5; the number of viable bacteria in the Lactobacillus reuteri powder is 2×10 11 cfu / g, the protective agent formula is skimmed milk powder: lactose: trehalose: fructooligosaccharides in mass ratio = 30:5:1:1. The above components are stirred and mix...

Embodiment 3

[0045] The preparation of embodiment 3 plectasomycin secretion type probiotics

[0046] Weigh each component of the following mass percentages: 45% of Plectasin-secreting Clostridium tyrobutyricum powder, 20% of Lactobacillus plantarum powder, 10% of Lactobacillus reuteri powder, 10% of fructooligosaccharides, and 15% of lactose . Among them, the number of live bacteria of the plectasin-secreting Clostridium tyrobutyricum powder was 3×10 9 cfu / g, the protective agent formula is according to the mass ratio Fumei powder: rice bran: corn starch = 80:10:10; the number of live bacteria of Lactobacillus plantarum powder is 1×10 11 cfu / g, the protective agent formula is skimmed milk powder in mass ratio: trehalose: sodium glutamate=20:10:5; the number of viable bacteria in the Lactobacillus reuteri powder is 5×10 10 cfu / g, the protective agent formula is skimmed milk powder: lactose: trehalose: fructooligosaccharide 20:10:10:5 by mass ratio. The above components are stirred and mi...

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Abstract

The invention belongs to the technical field of feed additives, and relates to a plectasin secretion type probiotic agent and a preparation method and application thereof. The plectasin secretory clostridium tyrobutyricum, the lactobacillus plantarum, the lactobacillus reuteri, the fructo-oligosaccharide and the lactose are scientifically matched to obtain the plectasin secretory type probiotic agent. The plectasin secreting type clostridium tyrobutyricum is matched with lactobacillus plantarum and lactobacillus reuteri for use, so that the drug resistance, double infection and the like causedby the use of antibiotics can be reduced, and the feed utilization rate, the natural defense mechanism of animals and the quality of animal products can be improved. The probiotic agent is tried outin a Guangdong tilapia mossambica improved variety farm, and the result shows that the probiotic agent has an obvious anti-streptococcal infection effect, the survival rate is increased, and the morbidity is obviously lower than that of an antibiotic group. The plectasin secretory probiotic agent has a wide application prospect in serving as or preparing aquatic animal feed additives.

Description

technical field [0001] The invention relates to the technical field of feed additives, and more specifically, to a plectasomycin-secreting probiotic agent and its preparation method and application. Background technique [0002] With the development of high-density and intensive farming of tilapia, the incidence of tilapia is getting higher and higher, and the prevention and treatment of streptococcal disease is more difficult, which poses a major threat to tilapia farming and has become a sustainable culture of tilapia. major constraint on development. The disease spreads quickly and has a high fatality rate. The outbreak of streptococcal disease is often related to the deterioration of water quality and high breeding density. There is still a lack of safe and effective drugs to control Streptococcus streptococcosis in tilapia. Tilapia is the main aquatic product export species, which is extremely important to my country's agricultural international trade. The outbreak of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/742A61K35/747A61P31/04A61P37/04A23K10/18A23K50/80C12R1/145C12R1/25C12R1/225
CPCC12N1/20A61K35/742A61K35/747A61P31/04A61P37/04A23K10/18A23K50/80A23V2400/173A23V2400/169A61K2300/00Y02A40/818
Inventor 曹丁赵培静张玲华李学优陈勉华甘祥武邓钧尹夏枫耿
Owner 广州市微生物研究所集团股份有限公司
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