Mouse intestinal roll specimen preparation device and method

Abstract

The invention discloses a mouse intestinal roll specimen preparation device and method, and relates to the technical field of animal specimen preparation. The mouse intestinal roll specimen preparation device comprises a winder and a wax strip, wherein the winder comprises a handle and a clamping base fixedly connected to one end of the handle, and an inserting hole is formed in the end, which isaway from the handle, of the clamping base; and one end of the wax strip is inserted into the inserting hole of the clamping base, and the other end of the wax strip extends out of the inserting holeof the clamping base. The mouse intestinal roll specimen preparation method is applied to the mouse intestinal roll specimen preparation device. The advantages are that 1, the Bouins liquid is used for carrying out pre-hardening treatment on the intestinal skin, so that the smoothness of the intestinal skin is favorably maintained, the pulling and pressing of the intestinal skin caused by the intestinal skin winding operation can be reduced, the operation difficulty during intestinal skin winding is reduced on the one hand, and the integrity of the intestinal morphological structure is guaranteed on the other hand; and 2, the preparation method of the intestinal roll is simple and easy to operate, consumes short time, can block endogenous or exogenous enzyme reaction in time, and is beneficial to ensuring the stability of protein in small intestine tissues;.

Description

technical field [0001] The invention relates to the technical field of animal specimen preparation, in particular to a device and method for preparing mouse intestinal roll specimens. Background technique [0002] The intestinal tract is an important organ for the transformation and absorption of organic matter, water, poisons, and drugs. Unlike other solid organ tissues, the intestinal tract presents a tubular cavity-like structure. The pathophysiological verification of intestinal tissue is an indispensable part of medical experimental research. In medical experiments, small animals such as rats, mice, and rabbits are usually used as the collection objects of intestinal samples. In the collection and fixation of samples, how to maintain the integrity of intestinal morphological structure and the stability of detection proteins has always been a technical problem that plagues researchers in the laboratory. [0003] Existing methods for fixing the intestinal tissues of expe...

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the deficiencies of the prior art, and provide a mouse intestinal roll sample preparation device and method, which solves the problem that the cross-section fixation method cannot completely present the intestine in the existing mouse intestinal tissue fixation method. The overall view of the tract tissue, the longitudinal section fixation method is difficult to operate and is likely to cause intestinal damage

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