91 results about "Mouse Intestine" patented technology

The invention discloses application of phlebopus portentosus polysaccharide in preparation of medicines or foods for regulating intestinal flora, reducing blood fat and reducing weight, and belongs to the technical field of biological medicines. The research on the effect of the phlebopus portentosus polysaccharide on obese model mice finds that the polysaccharide can reduce the occurrence of cardiovascular diseases such as atherosclerosis and the like, and has the effect of protecting the liver from being damaged. In addition, the liver injury state of the obese mouse is obviously relieved, the content of total short-chain fatty acid (SCFAs) of the obese mouse is increased, the species diversity of intestinal flora of the mouse is adjusted to a certain extent, and the colony composition is optimized.

The invention discloses a construction method and application of a probiotic engineering bacterium EcNpAm targeting animal intestinal tracts and improving inflammation. According to the present invention, the heterologous expression of the immunomodulatory protein Amuc1100 is successfully achieved through the gene engineering technology by using the Escherichia coli Niss1917 (EcN) as the host and using the anaerobic inducible plasmid pnirBMisL as the expression vector, and the novel engineering bacterium EcNpAm suitable for the animal intestinal anaerobic environment is constructed; furthermore, the expression efficiency of the target protein is represented by fusion expression of green fluorescent protein (GFP). The engineering bacterium is applied to prevention and treatment of animal intestinal inflammation, and successfully realizes targeted delivery of therapeutic protein to intestinal tracts of mice. Experiments show that EcNpAm can effectively relieve typical symptoms such as weight loss, colon shortening, pathological injury, colitis and the like of mice induced by dextran sodium sulfate (DSS) and protect integrity and functions of an intestinal barrier structure.

The invention relates to the technical field of biology, in particular to a composition, a culture medium and application of the culture medium in intestinal organ culture. The glucosamine hydrochloride is applied to culture of intestinal organs for the first time, organ proliferation and differentiation can be effectively promoted, and the number of formed organs is remarkably increased. On the basis, a three-dimensional culture system for staged time sequence induction in a postpartum newborn period, a child period and an adult period is constructed, and accurate construction of intestinal organs of the mice in different development periods is realized by combining dynamic regulation and control of an extracellular matrix and combinatorial optimization of growth factors; and an in-vitro model covering multiple time sequence stages is provided for intestinal development mechanism research and disease modeling.

The invention discloses a composition for synergistically regulating intestinal flora and application thereof. The composition comprises the following components in parts by weight: 10-80 parts of a cordyceps militaris extract, 2-40 parts of quercetin, 5-50 parts of prebiotics and 5-80 parts of nucleotide. The compositions provided by the invention supplement each other, have a synergistic effect, are compounded according to a scientific proportion, can improve the diversity of intestinal microorganisms of mice and the abundance of species, and can significantly improve intestinal inflammation symptoms in a mouse colitis model.

The mouse intestinal tumor cell injector comprises a handle, an injection device installed in the handle, and a radially telescopic support ring structure connected with the handle, tumor cells can be discharged from a first injection needle by operating a first push rod, and antibiotics can be discharged from a second injection needle by operating a second push rod; when the support ring structure is in a contracted state, the first injection needle and the second injection needle are located in the support ring structure; when the support ring structure is in an expanded support state, the needle heads of the first injection needle and the second injection needle can both extend to the outside of the outer edge of the support ring structure and are inserted into the mouse intestinal wall. The present application supports the mouse intestine by the support ring structure, supports the insertion of the first injection needle and the second injection needle into the mouse intestinal wall, ensures that only the mouse intestinal endothelium is punctured and the mouse intestinal canal is not punctured, ensures that tumor cells are injected into the mouse intestinal wall, and when the tumor cells are injected, antibiotics are also injected for anti-inflammatory treatment, which is beneficial to the recovery of the mouse intestinal puncture point.

The invention discloses a saccharomyces boulardii colon delivery system which comprises the following steps: the survival rate in gastric juice is enhanced, and the residence time in the colon is increased, so that the treatment effect of the saccharomyces boulardii is enhanced. Saccharomyces boulardii is taken as a research object, in order to improve the bioavailability of the Saccharomyces boulardii in gastrointestinal tracts and prolong the adhesion time of bacteria in intestinal tracts, a layer-by-layer electrostatic self-assembly method is adopted, PDAD (poly dimethyl diallyl ammonium chloride), PSS (sodium polystyrene sulfonate), CS (chitosan) and SA (sodium alginate) are taken as biological membranes to coat the Saccharomyces boulardii, and the coated Saccharomyces boulardii is prepared. Then a series of characterization is carried out, and the effect of the delivery system is verified through in-vitro artificial gastric juice tolerance tests and mouse intestinal colonization.

The invention belongs to the technical field of biology, and discloses a preparation method of an intestinal plexus patch, which comprises the following steps: killing an intestinal tract donor, fixing intestinal tissue segments of duodenum, jejunum, ileum or colon by using paraformaldehyde, and dehydrating to obtain dehydrated intestinal tissue segments; washing the dehydrated intestinal tissue section and removing mesentery, fat and Pari's lymph nodes attached to the jejunum, cutting an intestinal mass of about 4-6mm perpendicular to the intestinal tissue section, flatly laying the intestinal mass on a glass slide, dropwise adding a PBS (Phosphate Buffer Solution) of Triton X-100, fixing the intestinal mass by using a smooth surface of a pair of tweezers, stripping muscle tissues along the annular muscle direction, and drying the intestinal mass in a drying oven. The intestinal slices with intermuscular nerve plexus are obtained; finally, the intestinal piece with the intermuscular nerve plexus is cleaned, then fluorescent staining and piece sealing are conducted, and the mouse intestinal nerve plexus patch is obtained. The preparation method of the mouse intestinal nerve plexus patch has the advantages of being convenient, rapid and the like.

The application discloses application of Clostridium herbivorans in improving litter performance of mammals, and a probiotic preparation containing Clostridium herbivorans, wherein the viable bacterial count of Clostridium herbivorans in the probiotic preparation is 10 6 ~10 10 million / mL. After the Clostridium herbivorans colonizes in the intestinal tract of mice, the number of litters and the number of live litters of the mice can be improved.

The invention relates to the technical field of biology, in particular to a probiotic composition for reducing blood sugar and application thereof.The probiotic composition comprises bifidobacterium longum subsp. Longum FMBL B241770LS and bifidobacterium animalis subsp. Lactis FMBL B24304AHDM which are preserved in the China Center for Type Culture Collection on September 21, 2024 with the preservation number of CCTCC NO: M 20241993 and preserved in the China Center for Type Culture Collection on November 18, 2024 with the preservation number of CCTCC NO: M 20241993 with the preservation number of CCTCC NO: M 20241993 with the preservation number of CCTCC NO: M 20241993 with the preservation number of CCTCC NO: M 20241993 with the the preservation number is CTCC (China Type Culture Collection) NO: M 20242505; the compound has a good inhibition effect on pathogenic bacteria, can obviously reduce oral glucose tolerance and insulin tolerance of T2DM mice, reduce abnormal fluctuation of postprandial blood glucose, reduce fasting blood glucose, fasting insulin level and blood fat level, relieve insulin resistance, effectively regulate intestinal flora imbalance and promote generation of short-chain fatty acid in intestinal tracts of the T2DM mice.

The invention relates to the technical field of biological medicines, in particular to application of extracellular vesicles of Ackermann muciniphila in preparation of trichina control products. Experiments prove that by supplementing the AKK bacterium extracellular vesicles, the intestinal flora of a mouse can be remarkably improved, the immune balance of a host is regulated, and the pathological change of the intestinal tract of the mouse can be improved. Experimental results show that the AKK bacterium extracellular vesicles have a certain protection effect on trichina infected mice and have the advantages of being efficient, safe, easy to produce on a large scale and the like, and a brand new technical solution is provided for prevention and treatment of trichinosis.

A mouse intestinal pathological section embedding box is provided with an anti-collision block for fixing a Swiss roll, two non-woven fabric sheets, and a punching assembly for punching the Swiss roll. When in operation, the Swiss roll is fixed by the anti-collision block and the two non-woven fabric sheets, so that gaps are not easily generated in the formed wax block, and the subsequent sectioning and staining are more favorable, and the intestinal canal is not damaged. The method for making pathological sections comprises the following steps: separating the matched tool box from the embedding box body, punching the anti-collision block with a suitable punch, cleaning and rolling the mouse intestinal canal fixed by formalin into a Swiss roll; inserting the Swiss roll into the hole by using external force and flattening the protruding part of the intestinal canal tissue with a blade, placing the two layers of non-woven fabric to clamp the anti-collision block into the embedding box for tissue dehydration, peeling off the Swiss roll from the anti-collision block after dehydration and making a wax block; freezing and re-solidifying after heating and melting the paraffin, and then sectioning. The method can improve the overall morphological quality of pathological sections, and there is no phenomenon of structural loss.

The invention belongs to the technical field of intestinal flora sampling, and particularly relates to an experimental mouse intestinal flora sampling device which comprises a mounting cylinder, the side wall of the mounting cylinder is fixedly connected with a controller and a buzzer, the right side of the mounting cylinder is of an open structure, and the inner wall of the left side of the mounting cylinder is connected with an annular plate through a spring. And an injector is inserted into the right side of the mounting cylinder, a mounting frame is fixedly connected to the front side wall of the mounting cylinder, and the mounting frame is of a U-shaped structure. When the insertion head of the sampling device is inserted from the anus of the mouse, the diameter of the insertion head is reduced, so that the problems that the insertion head is too thick, the mouse is severely painful, the stress hormone level of the mouse is promoted to rise, and the intestinal flora composition is interfered are solved; and the insertion head can be expanded to a proper diameter, so that the problem that the insertion head is blocked by dietary fibers in intestinal tracts of mice is avoided.

The present application relates to the field of biotechnology, and particularly relates to a composition, a culture medium and application thereof in intestinal organoid culture. The present application first applies glucosamine hydrochloride to the culture of intestinal organoids, which can effectively promote organ proliferation and differentiation, and significantly increase the number of organ formation. On this basis, the present application constructs a three-dimensional culture system for postpartum neonatal, childhood and adult stages, and realizes the precise construction of mouse intestinal organoids at different development stages by combining dynamic regulation of extracellular matrix and optimization of growth factor combinations, thereby providing an in-vitro model covering multiple time sequences for intestinal development mechanism research and disease modeling.

The invention relates to a method for screening and identifying metabolites and florae with a human immune regulation effect, which comprises the following steps: (1) establishing immune system humanized mice with multiple intestinal types, and analyzing the change of the florae, the metabolites and the immune system through intestinal flora sequencing, serum metabolome sequencing, flow cytometry and transcriptome sequencing; (2) determining the correlation and sequence between flora related metabolites and the change of the immune system through bioinformatics analysis; and (3) confirming the regulation and control relationship between the candidate metabolites and the immune subgroups in the step (2) through biological experiments. By means of the screening method, the chemical small molecule metabolites for regulating and controlling the human immune cells in the normal physiological state can be screened on a large scale, and the chemical small molecule metabolites can be developed into immunotherapy drugs or used as cell therapy drug culture system components. The screened propionic acid can promote cytotoxicity of T cells and CAR-T and secretion of related cytokines in the aspect of function regulation of the T cells, and the metabolite 2-hydroxybutyric acid is highly positively correlated with the Treg cells and can promote generation and functions of the Treg cells.

The invention discloses application of bitter gourd exosomes in regulating intestinal flora. The method comprises the following steps: establishing an Alzheimer's disease mouse model, injecting the extracted balsam pear exosome into the mouse body in a gavage manner to serve as an experimental group drug, taking normal saline as a model negative control group, detecting the change of the intestinal flora of the AD mouse by applying a 16S amplicon sequencing principle, and detecting the change of the intestinal flora metabolite of the AD mouse by applying an LS-MS technology. An open field experiment, a nesting experiment and a water maze experiment are adopted to verify the treatment effect of the balsam pear exosome on AD mouse intestinal flora and metabolic level changes thereof. Results prove that the bitter gourd exosome extracted by the invention can effectively improve the intestinal flora micro-ecology of AD mice; the compound can be used for preparing a microecological preparation for regulating intestinal flora of neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, Huntington's disease, amyotrophic lateral sclerosis, different types of spinal cerebellar ataxia and the like and diseases such as cerebral apoplexy, cerebral injury, epilepsy, tumor and the like.

The invention discloses an application of gelatinized intestinal epithelial cells in preparation of drugs for treating inflammatory bowel disease, the gelatinized intestinal epithelial cells take mouse intestinal epithelial cells as cores, poly (ethylene glycol) diacrylate, a photoinitiator and poly-L-lysine permeate into the cells through low-temperature freeze thawing, and an intracellular porous hydrogel skeleton is formed through ultraviolet crosslinking. The gelated intestinal epithelial cell has a strong cfDNA and inflammatory factor removal function, has good biocompatibility, has no direct or indirect toxic effect on a human body, has no potential toxicity, and can be used for treating inflammatory bowel diseases.

The mouse intestine roll specimen preparation device comprises a box body, a box cover, a sliding plate and an insertion column, the top face of the box body is open, and the box cover is connected to the opening of the top face of the box body in an inserted mode; the sliding plate is located on the inner bottom face of the box body, the side face of the sliding plate is slidably connected with the inner side wall of the box body, and the sliding plate slides in the vertical direction. The upper surface of the sliding plate is symmetrically provided with two rows of jacks, each row of jacks comprises three jacks, the lower ends of the insertion columns are provided with insertion blocks, and the insertion blocks are connected with the holes in a pluggable mode. According to the mouse intestine dehydration device, after the mouse intestine is completely dehydrated, the sliding plate and the insertion columns are moved upwards to be completely away from formalin solution, and the insertion columns are pulled out of the insertion holes, so that the mouse intestine can be conveniently taken down from the insertion columns, and subsequent sample manufacturing is facilitated; the mouse intestine is in a specific shape, and after the mouse intestine is soaked in a formalin solution and dehydrated, the mouse intestine has a plurality of straight sections, so that the later processing and manufacturing of specimens are facilitated.

The present application relates to the field of microbial sampling and detection, and particularly relates to a mouse experimental E. coli sampling and detection device. The technical problem to be solved is that when sampling and detecting E. coli in the intestinal tract of a mouse, the attention of manual operation needs to be highly concentrated, and it is easy to cause physical and mental fatigue, and the intestinal tract will be damaged if a little carelessness. In addition, the existing technology cannot remove the fat tissue existing on the outer surface of the intestinal tract, thereby affecting the sampling and detection result. The technical scheme is as follows: a mouse experimental E. coli sampling and detection device, comprising a sampling box and a fixing assembly. The first clamp block and the second clamp block are respectively arranged on the left end and the right end of the intestinal tract of the mouse to fix the intestinal tract, the injection tube is used to inject sterile water into the inside of the intestinal tract to flush, the cleaning of the intestinal tract of the mouse is completed, the pressure of manual operation is reduced, and the accidental damage of the intestinal tract of the mouse caused by manual operation is reduced.

The present application relates to a kind of crude extract of magnolia flower and its preparation method and application, the preparation method of the crude extract of magnolia flower includes: fresh magnolia flower is sequentially dried, pulverized, screened and then obtains magnolia flower powder;The required quality of magnolia flower powder is weighed, under the conditions of extraction temperature 40-80 ℃, extraction pressure 10-30 Mpa, entrainer concentration 40%-80%, entrainer flow rate 0.5-2.5 ml / min, supercritical CO2 extraction is carried out for 1-3 hours to obtain the crude extract of magnolia flower;The crude extract of magnolia flower is rotary evaporated to dryness, and the crude extract of magnolia flower is obtained.The present application uses magnolia flower as raw material, uses supercritical CO2 extraction technology to prepare the crude extract of magnolia flower, uses excessive antibiotics to induce intestinal flora disorder of mouse, establishes weak mouse model of spleen deficiency, and by using different concentrations of the crude extract of magnolia flower to the mouse is treated by gavage, find that the crude extract of magnolia flower can balance its intestinal flora, adjust the weak state of mouse of spleen deficiency, and improve immunity.

The invention discloses bifidobacterium breve for regulating metabolism of vitamin A in intestinal flora and application of the bifidobacterium breve, and belongs to the field of microorganisms. According to the bifidobacterium breve CCFM1489 disclosed by the invention, vitamin A can be subjected to external metabolism to generate retinoic acid; corneal injury and conjunctival goblet cell injury of mice with xerophthalmia are relieved; the RA level in serum of the dry-eye mouse is improved; the levels of IL-6, IL-17 and TNF-alpha in serum of the mouse with the dry eye syndrome are reduced, the intestinal flora of the mouse with the dry eye syndrome is regulated, and the abundance of beneficial bacteria is increased. Therefore, the bifidobacterium breve CCFM1489 has a huge application prospect in preparation of products for preventing and / or relieving xerophthalmia.

The invention relates to the field of biological medicine, in particular to application of a quinic acid derivative. The quinic acid derivative comprises one or two of 3-O-p-coumaroyl quinic acid and 4-O-p-coumaroyl quinic acid, and the quinic acid derivative comprises one or two of 3-O-p-coumaroyl quinic acid and 4-O- The applications include treatment and / or prevention of depression. The quinic acid derivatives 3-O-p-coumaroyl quinic acid and 4-O-p-coumaroyl quinic acid can be used for improving the behavioristics of depressive mice; the compound can significantly increase dopamine and noradrenaline in the brain of a depressive mouse, reduce the levels of inflammatory factors IL-6 and TNF-alpha in the brain of the depressive mouse, increase the intestinal microbial diversity of the depressive mouse and significantly increase the BDNF level in the brain of the depressive mouse, and can be used as a drug for treating and / or preventing depression.

The invention belongs to the technical field of medicine preparation, discloses a preparation method of a composite microbial preparation, focuses on a specific co-disease group of'depression-constipation-sleep disorder 'for the first time, provides a single-preparation comprehensive solution for comprehensively adjusting'intestine-brain axis' through multi-strain synergy, and fills the market blank. Constipation indexes are as follows: the intestinal tract propulsion rate of mice is increased by 50%, and the discharge time of first black feces is shortened by 30%. The depression index is as follows: in a forced swimming experiment, the immobile time is reduced by 40%, and the sweet water preference is obviously increased. Sleep indexes are as follows: the total sleep time is prolonged by 25%, and the sleep latency period is shortened by 20%. Biochemical indexes are as follows: the level of inflammatory factor IL-6 in serum is reduced by 60%, and the content of 5-HT and GABA in colon tissue is remarkably increased.

This application relates to the use of 12-ketolithocholic acid in the preparation of a drug for preventing or treating acute ischemic intestinal injury. This application attempts to use 12-ketolithocholic acid (12-KLCA) for the prevention or treatment of acute ischemic intestinal injury, and the application has been validated in a constructed classic intestinal ischemia-reperfusion model. The validation results show that 12-KLCA significantly improves intestinal histopathological damage induced by intestinal ischemia-reperfusion in mice, increases the survival rate of mice, and reduces the protein expression level of the apoptotic factor activating caspase-3. The effect is significant, safe, non-toxic, and has minimal side effects.

The invention belongs to the field of medicines, and particularly relates to fructus amomi hainanensis polysaccharide as well as a preparation method and application thereof. According to the method, the active ingredients of the polysaccharide are extracted by taking the fructus amomi hainanensis as a raw material, HPGPC, UV, FT-IR and monosaccharide composition analysis are adopted for structural analysis, the structural formula of the fructus amomi hainanensis polysaccharide is obtained, the structural characteristics of the fructus amomi hainanensis polysaccharide are defined, and a material foundation is laid for pharmacodynamic activity research of the fructus amomi hainanensis polysaccharide. After treatment with the fructus amomi hainanensis polysaccharide, the DAI score of a mouse with ulcerative colitis and the pathological damage degree of colon tissue are obviously reduced, the levels of proinflammatory factors IL-1beta, TNF-alpha and IL-6 are obviously reduced, the level of an anti-inflammatory factor IL-10 is obviously increased, the expression reduction of tight junction protein caused by DSS can be recovered, and the ulcerative colitis can be inhibited. Disorder of metabolites in mice with ulcerative colitis can be regulated, and reduction of diversity and abundance of intestinal microorganisms of the UC mice can be reversed.

The invention provides an application of exemestane or a derivative thereof in preparation of a medicine for preventing radioactive intestinal injury, and also provides a pharmaceutical composition for preventing radioactive intestinal injury, which consists of exemestane or a derivative thereof and pharmaceutically acceptable auxiliary materials. According to the invention, exemestane is screened and locked at high throughput from 3067 compound libraries approved by FDA for the first time. A radioactive intestinal injury mouse model verifies that 6.25 mg / kg exemestane is given for intragastric therapy 2 hours before the whole body is irradiated by 8 Gy, and the survival rate of the mouse can be remarkably increased. HE histology shows that the integrity of intestinal crypt-villus structures of mice in an exemestane administration group is recovered, the empty degree of marrow cavities is relieved, and spleen injury is relieved. The results prove that exemestane can protect intestinal tracts, hematopoietic organs and immune organs at the same time at the whole animal level, and the exemestane is a rare multi-organ integrated protection small molecule compound so far. The invention provides a new basis for exemestane or the derivative thereof to prevent radioactive intestinal injury.

The invention provides application of difructose anhydride in improving intestinal inflammation, and by constructing a DSS colitis mouse model, the DFA is proved to be capable of remarkably improving the disease activity index, inhibiting weight loss and relieving splenomegaly and colon shortening. The intestinal flora analysis shows that the DFA intervention obviously increases the relative abundance of bifidobacterium and lactobacillus in the intestinal tract of the mouse, reduces the abundance of pathogenic bacterium desulfurization vibrio and promotes the generation of short-chain fatty acid. The invention further develops a synbiotics capsule containing DFA, and a sodium alginate-chitosan dual embedding technology is adopted. The invention systematically discloses an action mechanism of improving intestinal inflammation through flora-metabolism-immunization in a multi-target manner by DFA, and provides a novel functional preparation for IBD treatment.

The invention discloses an application of an intestinal organ model in nano-material intestinal toxicity evaluation, which comprises the following steps: mixing a mouse intestinal crypt fragment with matrigel, inoculating the mixture to a pore plate, adding an organ culture medium after the matrigel is solidified, adding a nano-material into a culture system, and carrying out subculture; acquiring the form and growth index data of intestinal organs, and evaluating the toxic and side effects of the nano material according to the acquired data result. Compared with a traditional 2D model, the nanometer material intestinal toxicity evaluation method based on the 3D organ-like model is more real and reliable in evaluation result.

The invention belongs to the technical field of molecular biology, and discloses application of DDX24 in maintaining the homeostasis of an intestinal vascular barrier. According to the present invention, the low expression of the intestinal microvascular endothelial DDX24 in the inflammatory bowel disease (IBD) is firstly found so as to construct the adult endothelial cell specific DDX24 induced knockout mouse model, the homeostasis of the intestinal vascular barrier (GVB) of the mouse is imbalanced, and the mouse suffers from spontaneous enteritis, such that the intestinal microvascular endothelial cell DDX24 is the important molecule for maintaining the intestinal homeostasis; and mechanism research finds that the intestinal microvascular endothelial cell DDX24 inhibits cell senescence to protect the GVB steady state. The research discloses an important mechanism of DDX24 for protecting GVB homeostasis by inhibiting intestinal microvascular endothelial cell senescence for the first time, and provides a strategy and basis for clinical diagnosis and treatment of IBD vascular barrier.

The invention relates to application of taurine in treatment of neonatal necrotizing enterocolitis. Experiments prove that taurine can improve intestinal inflammation of NEC mice and promote intestinal epithelial cell proliferation. The participation of taurine in the regulation and control of NEC inflammation is possibly related to a TLR4 / NF-kappa B signal. Molecular docking data shows that taurine interferes with TLR4 / MD2 protein interaction, and then TLR4 downstream inflammation signal activation is inhibited. NEC inflammation is relieved by inhibiting a TLR4 / NF-kappa B signal, and repair after NEC intestinal epithelium injury is promoted. The application of taurine in neonatal necrotizing enterocolitis is explored from animal and molecular levels, and a theoretical basis is provided for prevention and treatment of neonatal necrotizing enterocolitis.

The invention belongs to the field of health food, and particularly relates to a dendrobium officinale fermentation liquor composition with an anti-inflammatory effect and a preparation method thereof.The composition is prepared from 30-40 parts of dendrobium officinale fermentation liquor, 15-25 parts of cyclocarya paliurus juice, 10-20 parts of kiwi fruit juice, 8-12 parts of sugarcane juice, 3-5 parts of polydextrose, 2-4 parts of lemon juice and 0.5-1 part of mint. The invention further optimizes a process method for fermenting the dendrobium officinale. According to the dendrobium officinale fermentation liquor composition, the anti-inflammatory effect is remarkably improved, the immunocompetence in a mouse body can be adjusted, the remarkable protection effect on the intestinal tract of the mouse is achieved, and the total phenol content and the total sugar content of the dendrobium officinale fermentation liquor can be remarkably improved through the dendrobium officinale fermentation process.