123 results about "Mouse Intestine" patented technology

The invention relates to application of 12-ketolithocholic acid in preparation of a medicine for preventing or treating acute ischemic intestinal injury. According to the application, 12-ketolithocholic acid (12-KLCA) is used for preventing or treating acute ischemic intestinal injury and is verified on a constructed classical intestinal ischemia reperfusion model, and a verification result shows that the 12-KLCA obviously improves intestinal histopathologic injury induced by intestinal ischemia reperfusion of a mouse, improves the survival rate of the mouse, and has a good application prospect in prevention or treatment of acute ischemic intestinal injury. The protein expression level of the apoptosis factor activated caspase-3 is reduced, the effect is obvious, safety and non-toxicity are achieved, and the side effect is small.

The invention relates to the field of microorganism sampling detection, in particular to escherichia coli sampling detection equipment for mouse experiments. The technical problem to be solved is that when mouse intestinal escherichia coli is sampled and detected, attention needs to be highly concentrated during manual operation, physical and mental exhaustion is easily caused, and the intestinal tract is damaged by slight carelessness. In addition, in the prior art, adipose tissue existing on the outer surface of the intestinal tract cannot be removed, and therefore the sampling detection result is affected. According to the technical scheme, the escherichia coli sampling and detecting equipment for the mouse experiment comprises a sampling box, a fixing assembly and the like; and a fixing assembly is mounted in the sampling box. According to the device, the left end and the right end of the intestinal tract of a mouse are fixed through the first clamping block and the second clamping block respectively, sterile water is injected into the intestinal tract of the mouse through an injection pipe for flushing, and cleaning of the intestinal tract of the mouse is completed, so that the pressure of manual operation is relieved, and meanwhile accidental damage to the intestinal tract of the mouse caused by manual operation is reduced.

The invention discloses a preparation method of cordyceps sobolifera polysaccharide and application of the cordyceps sobolifera polysaccharide in relieving acute chemical liver injury. Monosaccharide of the cordyceps sobolifera polysaccharide (abbreviated as CCPW) is composed of glucose, mannose and galactose according to the molar ratio of 8.918: 1: 0.356, and the relative molecular mass is 2.62 * 10 < 4 > Da. Efficacy experiments show that the cordyceps sobolifera homogeneous polysaccharide CCPW can significantly inhibit increase of ALT and AST in a CCl4-induced acute chemical liver injury model mouse, effectively improve the degree of liver cell disruption and swelling, relieve liver inflammatory response, relieve CCl4-induced mouse liver tissue injury, protect mouse intestinal barriers, recover intestinal microbial balance and have obvious liver protection activity. The cordyceps sobolifera polysaccharide CCPW is clear in composition and remarkable in effect, and has wide development and application prospects in the aspect of relieving acute chemical liver injury.

The invention discloses application of phlebopus portentosus polysaccharide in preparation of medicines or foods for regulating intestinal flora, reducing blood fat and reducing weight, and belongs to the technical field of biological medicines. The research on the effect of the phlebopus portentosus polysaccharide on obese model mice finds that the polysaccharide can reduce the occurrence of cardiovascular diseases such as atherosclerosis and the like, and has the effect of protecting the liver from being damaged. In addition, the liver injury state of the obese mouse is obviously relieved, the content of total short-chain fatty acid (SCFAs) of the obese mouse is increased, the species diversity of intestinal flora of the mouse is adjusted to a certain extent, and the colony composition is optimized.

The invention discloses a construction method and application of a GSDMB intestinal epithelium specific knock-in mouse model. The construction method comprises the following steps: (1) designing gRNA according to an ROSA26 gene, constructing a Donor vector containing a GSDMB gene, introducing the gRNA and the Donor vector and Cas mRNA into a fertilized egg of a receptor mouse, transplanting the fertilized egg and Cas mRNA into a pseudopregnant mouse body for reproduction, and screening out a GSDMB gene knock-in F0-generation mouse; (2) mating and breeding the F0 generation mouse and a wild type mouse, and screening out an F1 heterozygote mouse with GSDMB knocked in; (3) carrying out inbreeding propagation on the F1 heterozygote mouse, and screening out an F2 homozygote mouse in which GSDMB is knocked; (4) the F2 homozygote mouse and the intestinal epithelium specific Vilin-Cre transgenic mouse are subjected to mating breeding, and a heterozygote mouse with GSDMB intestinal epithelium specific knock-in is screened out; and (5) carrying out inbreeding propagation on the heterozygote mouse with the GSDMB intestinal epithelium specific knock-in, and screening out the homozygote mouse with the GSDMB intestinal epithelium specific knock-in.

The invention belongs to the technical field of biology, and particularly relates to a construction method and application of a model with fusobacterium nucleatum damaged mouse intestinal tract organoid, and the construction method comprises the following steps: separating mouse intestinal tract organoid; obtaining a culture supernatant with fusobacterium nucleatum; obtaining a cell-free extract with fusobacterium nucleatum; viable bacteria with fusobacterium nucleatum are obtained; and treating the mouse intestinal organ with the fusobacterium nucleatum culture supernatant or the fusobacterium nucleatum cell-free extract or the fusobacterium nucleatum viable bacteria to obtain the model of the mouse intestinal organ damaged by the fusobacterium nucleatum. The construction method of the organ-like model with the fusobacterium nucleatum damaged intestinal tract is simple, operability and convenience can be increased for further research on a regulation mechanism of the fusobacterium nucleatum damaged intestinal tract, and a reliable and accurate research tool is provided for gastrointestinal diseases such as inflammatory bowel diseases and colorectal cancer.

The invention discloses a construction method and application of a probiotic engineering bacterium EcNpAm targeting animal intestinal tracts and improving inflammation. According to the present invention, the heterologous expression of the immunomodulatory protein Amuc1100 is successfully achieved through the gene engineering technology by using the Escherichia coli Niss1917 (EcN) as the host and using the anaerobic inducible plasmid pnirBMisL as the expression vector, and the novel engineering bacterium EcNpAm suitable for the animal intestinal anaerobic environment is constructed; furthermore, the expression efficiency of the target protein is represented by fusion expression of green fluorescent protein (GFP). The engineering bacterium is applied to prevention and treatment of animal intestinal inflammation, and successfully realizes targeted delivery of therapeutic protein to intestinal tracts of mice. Experiments show that EcNpAm can effectively relieve typical symptoms such as weight loss, colon shortening, pathological injury, colitis and the like of mice induced by dextran sodium sulfate (DSS) and protect integrity and functions of an intestinal barrier structure.

The invention discloses Kluyveromyces marxianus KUST3801 and application thereof in relieving constipation, and the preservation number of the Kluyveromyces marxianus KUST3801 is CGMCC (China General Microbiological Culture Collection Center) NO.32767. The Kluyveromyces marxianus strain is classified and named as Kluyveromyces marxianus, and the Kluyveromyces marxianus strain is named as Kluyveromyces marxianus. The sequence of the 16SrRNA is as shown in SEQ ID NO. 1; the composition is used for improving constipation symptoms. After the kluyveromyces marxianus KUST3801 is applied, the excrement water content and the small intestine propulsion rate of a mouse with ICR constipation induced by loperamide hydrochloride are remarkably improved, and the defecation frequency and the defecation number within the same time are remarkably increased; intestinal mucosa tissues of constipation mice can be protected, and intestinal mucosa injury is relieved; the concentration of SP and MTL is obviously improved, and the concentration of VIP is reduced; iL-6 concentration is reversed, and inflammatory response is relieved; the concentrations of acetic acid, butyric acid, isobutyric acid and isovaleric acid in the short-chain fatty acid are obviously higher than those of mice with constipation, and the composition has a relatively strong effect of relieving constipation symptoms.

The invention relates to the technical field of biology, in particular to a composition, a culture medium and application of the culture medium in intestinal organ culture. The glucosamine hydrochloride is applied to culture of intestinal organs for the first time, organ proliferation and differentiation can be effectively promoted, and the number of formed organs is remarkably increased. On the basis, a three-dimensional culture system for staged time sequence induction in a postpartum newborn period, a child period and an adult period is constructed, and accurate construction of intestinal organs of the mice in different development periods is realized by combining dynamic regulation and control of an extracellular matrix and combinatorial optimization of growth factors; and an in-vitro model covering multiple time sequence stages is provided for intestinal development mechanism research and disease modeling.

The invention discloses a composition for synergistically regulating intestinal flora and application thereof. The composition comprises the following components in parts by weight: 10-80 parts of a cordyceps militaris extract, 2-40 parts of quercetin, 5-50 parts of prebiotics and 5-80 parts of nucleotide. The compositions provided by the invention supplement each other, have a synergistic effect, are compounded according to a scientific proportion, can improve the diversity of intestinal microorganisms of mice and the abundance of species, and can significantly improve intestinal inflammation symptoms in a mouse colitis model.

The mouse intestinal tumor cell injector comprises a handle, an injection device installed in the handle, and a radially telescopic support ring structure connected with the handle, tumor cells can be discharged from a first injection needle by operating a first push rod, and antibiotics can be discharged from a second injection needle by operating a second push rod; when the support ring structure is in a contracted state, the first injection needle and the second injection needle are located in the support ring structure; when the support ring structure is in an expanded support state, the needle heads of the first injection needle and the second injection needle can both extend to the outside of the outer edge of the support ring structure and are inserted into the mouse intestinal wall. The present application supports the mouse intestine by the support ring structure, supports the insertion of the first injection needle and the second injection needle into the mouse intestinal wall, ensures that only the mouse intestinal endothelium is punctured and the mouse intestinal canal is not punctured, ensures that tumor cells are injected into the mouse intestinal wall, and when the tumor cells are injected, antibiotics are also injected for anti-inflammatory treatment, which is beneficial to the recovery of the mouse intestinal puncture point.

The invention discloses a saccharomyces boulardii colon delivery system which comprises the following steps: the survival rate in gastric juice is enhanced, and the residence time in the colon is increased, so that the treatment effect of the saccharomyces boulardii is enhanced. Saccharomyces boulardii is taken as a research object, in order to improve the bioavailability of the Saccharomyces boulardii in gastrointestinal tracts and prolong the adhesion time of bacteria in intestinal tracts, a layer-by-layer electrostatic self-assembly method is adopted, PDAD (poly dimethyl diallyl ammonium chloride), PSS (sodium polystyrene sulfonate), CS (chitosan) and SA (sodium alginate) are taken as biological membranes to coat the Saccharomyces boulardii, and the coated Saccharomyces boulardii is prepared. Then a series of characterization is carried out, and the effect of the delivery system is verified through in-vitro artificial gastric juice tolerance tests and mouse intestinal colonization.

The invention belongs to the technical field of biology, and discloses a preparation method of an intestinal plexus patch, which comprises the following steps: killing an intestinal tract donor, fixing intestinal tissue segments of duodenum, jejunum, ileum or colon by using paraformaldehyde, and dehydrating to obtain dehydrated intestinal tissue segments; washing the dehydrated intestinal tissue section and removing mesentery, fat and Pari's lymph nodes attached to the jejunum, cutting an intestinal mass of about 4-6mm perpendicular to the intestinal tissue section, flatly laying the intestinal mass on a glass slide, dropwise adding a PBS (Phosphate Buffer Solution) of Triton X-100, fixing the intestinal mass by using a smooth surface of a pair of tweezers, stripping muscle tissues along the annular muscle direction, and drying the intestinal mass in a drying oven. The intestinal slices with intermuscular nerve plexus are obtained; finally, the intestinal piece with the intermuscular nerve plexus is cleaned, then fluorescent staining and piece sealing are conducted, and the mouse intestinal nerve plexus patch is obtained. The preparation method of the mouse intestinal nerve plexus patch has the advantages of being convenient, rapid and the like.

Disclosed herein is a custom platform called NSC-seq that can be used for cell lineage and cell division tracking in a temporal fashion but also with single-cell resolution. With simultaneous integration of single-cell transcriptomics with lineage barcoding and temporal recording information, NSC-seq facilitates the generation of multidimensional datasets for elucidating functional heterogeneity and clonal events in vivo. We apply this platform (i) to decipher lineage branching of mouse embryonic development, (ii) to record clonal dynamics of the adult intestinal epithelium, and (iii) to track clonal composition of the mouse intestinal tumors. In addition, we apply NSC-seq to assess a comprehensive gene expression phenotype for individual gene knockout at the single-cell level using existing whole-genome CRISPR knockout screening plasmid libraries. Overall, NSC-seq enables in vivo temporal recording of mammalian development and cancer at single-cell resolution.

The invention provides application of a compound azelaic acid in preparation of a medicine for preventing and treating sepsis acute lung injury. Animal experiments show that azelaic acid is highly enriched in intestinal tracts and lungs of mice with ketogenic diet and is closely related to the occurrence and development process of sepsis acute lung injury. The compound azelaic acid can obviously inhibit lung inflammatory cell infiltration, inflammatory factor generation and lung cell apoptosis on the animal level, and can be used for preparing the medicine for preventing and treating acute lung injury caused by sepsis.

The application discloses application of Clostridium herbivorans in improving litter performance of mammals, and a probiotic preparation containing Clostridium herbivorans, wherein the viable bacterial count of Clostridium herbivorans in the probiotic preparation is 10 6 ~10 10 million / mL. After the Clostridium herbivorans colonizes in the intestinal tract of mice, the number of litters and the number of live litters of the mice can be improved.

The invention relates to the technical field of biology, in particular to a probiotic composition for reducing blood sugar and application thereof.The probiotic composition comprises bifidobacterium longum subsp. Longum FMBL B241770LS and bifidobacterium animalis subsp. Lactis FMBL B24304AHDM which are preserved in the China Center for Type Culture Collection on September 21, 2024 with the preservation number of CCTCC NO: M 20241993 and preserved in the China Center for Type Culture Collection on November 18, 2024 with the preservation number of CCTCC NO: M 20241993 with the preservation number of CCTCC NO: M 20241993 with the preservation number of CCTCC NO: M 20241993 with the preservation number of CCTCC NO: M 20241993 with the the preservation number is CTCC (China Type Culture Collection) NO: M 20242505; the compound has a good inhibition effect on pathogenic bacteria, can obviously reduce oral glucose tolerance and insulin tolerance of T2DM mice, reduce abnormal fluctuation of postprandial blood glucose, reduce fasting blood glucose, fasting insulin level and blood fat level, relieve insulin resistance, effectively regulate intestinal flora imbalance and promote generation of short-chain fatty acid in intestinal tracts of the T2DM mice.

The invention relates to the technical field of biological medicines, in particular to application of extracellular vesicles of Ackermann muciniphila in preparation of trichina control products. Experiments prove that by supplementing the AKK bacterium extracellular vesicles, the intestinal flora of a mouse can be remarkably improved, the immune balance of a host is regulated, and the pathological change of the intestinal tract of the mouse can be improved. Experimental results show that the AKK bacterium extracellular vesicles have a certain protection effect on trichina infected mice and have the advantages of being efficient, safe, easy to produce on a large scale and the like, and a brand new technical solution is provided for prevention and treatment of trichinosis.

A mouse intestinal pathological section embedding box is provided with an anti-collision block for fixing a Swiss roll, two non-woven fabric sheets, and a punching assembly for punching the Swiss roll. When in operation, the Swiss roll is fixed by the anti-collision block and the two non-woven fabric sheets, so that gaps are not easily generated in the formed wax block, and the subsequent sectioning and staining are more favorable, and the intestinal canal is not damaged. The method for making pathological sections comprises the following steps: separating the matched tool box from the embedding box body, punching the anti-collision block with a suitable punch, cleaning and rolling the mouse intestinal canal fixed by formalin into a Swiss roll; inserting the Swiss roll into the hole by using external force and flattening the protruding part of the intestinal canal tissue with a blade, placing the two layers of non-woven fabric to clamp the anti-collision block into the embedding box for tissue dehydration, peeling off the Swiss roll from the anti-collision block after dehydration and making a wax block; freezing and re-solidifying after heating and melting the paraffin, and then sectioning. The method can improve the overall morphological quality of pathological sections, and there is no phenomenon of structural loss.

The invention belongs to the technical field of intestinal flora sampling, and particularly relates to an experimental mouse intestinal flora sampling device which comprises a mounting cylinder, the side wall of the mounting cylinder is fixedly connected with a controller and a buzzer, the right side of the mounting cylinder is of an open structure, and the inner wall of the left side of the mounting cylinder is connected with an annular plate through a spring. And an injector is inserted into the right side of the mounting cylinder, a mounting frame is fixedly connected to the front side wall of the mounting cylinder, and the mounting frame is of a U-shaped structure. When the insertion head of the sampling device is inserted from the anus of the mouse, the diameter of the insertion head is reduced, so that the problems that the insertion head is too thick, the mouse is severely painful, the stress hormone level of the mouse is promoted to rise, and the intestinal flora composition is interfered are solved; and the insertion head can be expanded to a proper diameter, so that the problem that the insertion head is blocked by dietary fibers in intestinal tracts of mice is avoided.

The present invention provides a preparation method for extracting volatile oil of Chimonanthus chinensis leaves by ultrasonic-assisted steam distillation and its application in improving ulcerative colitis drugs. The method includes crushing Chimonanthus chinensis leaves to a specific particle size and then performing ultrasonic treatment and steam distillation under optimized conditions, and finally achieving 2.5% to 4% high-efficiency extraction. Volatile oil is rich in phellandrene, α-terpineol and (+)-limonene, accounting for more than 50% of the total mass. Pharmacological experiments show that this volatile oil can significantly reduce the levels of pro-inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) in mouse serum, increase anti-inflammatory factor interleukin 10 (IL-10); increase the expression of tight junction protein ZO-1, protect the integrity of mouse intestinal epithelial barrier; and significantly downregulate the expression of proline hydroxylase 3 factor of HIF-1 pathway to inhibit inflammation.

The present application relates to the field of biotechnology, and particularly relates to a composition, a culture medium and application thereof in intestinal organoid culture. The present application first applies glucosamine hydrochloride to the culture of intestinal organoids, which can effectively promote organ proliferation and differentiation, and significantly increase the number of organ formation. On this basis, the present application constructs a three-dimensional culture system for postpartum neonatal, childhood and adult stages, and realizes the precise construction of mouse intestinal organoids at different development stages by combining dynamic regulation of extracellular matrix and optimization of growth factor combinations, thereby providing an in-vitro model covering multiple time sequences for intestinal development mechanism research and disease modeling.

The invention relates to a method for screening and identifying metabolites and florae with a human immune regulation effect, which comprises the following steps: (1) establishing immune system humanized mice with multiple intestinal types, and analyzing the change of the florae, the metabolites and the immune system through intestinal flora sequencing, serum metabolome sequencing, flow cytometry and transcriptome sequencing; (2) determining the correlation and sequence between flora related metabolites and the change of the immune system through bioinformatics analysis; and (3) confirming the regulation and control relationship between the candidate metabolites and the immune subgroups in the step (2) through biological experiments. By means of the screening method, the chemical small molecule metabolites for regulating and controlling the human immune cells in the normal physiological state can be screened on a large scale, and the chemical small molecule metabolites can be developed into immunotherapy drugs or used as cell therapy drug culture system components. The screened propionic acid can promote cytotoxicity of T cells and CAR-T and secretion of related cytokines in the aspect of function regulation of the T cells, and the metabolite 2-hydroxybutyric acid is highly positively correlated with the Treg cells and can promote generation and functions of the Treg cells.

The invention provides application of P.distassonis ATCC: 8503 in treatment of intestinal ischemia reperfusion injury, and belongs to the technical field of microorganisms. According to the application disclosed by the invention, the P.distaxis ATCC: 8503 is used for treating the intestinal ischemia-reperfusion injury, the treatment effect of the P.distaxis ATCC: 8503 is detected, and a result proves that the P.distaxis ATCC: 8503 can be used for inhibiting the pathological morphology injury of intestinal tissues induced by the intestinal ischemia-reperfusion injury, reducing the content of FITC (Fluorescein Isothiocyanate) and LPS (Lipopolysaccharide) in serum and reducing the permeability of an intestinal barrier, so that the P.distaxis ATCC: 8503 can be used for treating the intestinal ischemia-reperfusion injury. The intestinal barrier function injury induced by the intestinal ischemia reperfusion injury is relieved, and the endotoxin displacement and the kidney injury induced by the intestinal ischemia reperfusion injury of the mouse are relieved.

The invention discloses application of bitter gourd exosomes in regulating intestinal flora. The method comprises the following steps: establishing an Alzheimer's disease mouse model, injecting the extracted balsam pear exosome into the mouse body in a gavage manner to serve as an experimental group drug, taking normal saline as a model negative control group, detecting the change of the intestinal flora of the AD mouse by applying a 16S amplicon sequencing principle, and detecting the change of the intestinal flora metabolite of the AD mouse by applying an LS-MS technology. An open field experiment, a nesting experiment and a water maze experiment are adopted to verify the treatment effect of the balsam pear exosome on AD mouse intestinal flora and metabolic level changes thereof. Results prove that the bitter gourd exosome extracted by the invention can effectively improve the intestinal flora micro-ecology of AD mice; the compound can be used for preparing a microecological preparation for regulating intestinal flora of neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, Huntington's disease, amyotrophic lateral sclerosis, different types of spinal cerebellar ataxia and the like and diseases such as cerebral apoplexy, cerebral injury, epilepsy, tumor and the like.

The present invention discloses Parabacteroides distasonis NSP007 for alleviating insulin resistance and its application, belonging to the field of microbial technology. Parabacteroides distasonis NSP007 of the present invention can inhibit the weight gain and body fat accumulation of IR mice without affecting food intake; improve the glucose tolerance and insulin sensitivity of IR mice, while reducing the serum insulin and insulin resistance index of mice; regulate the lipid metabolism disorder of IR mice and relieve liver tissue damage; improve the intestinal barrier function of IR mice. The Parabacteroides distasonis NSP007 of the present invention is used for preparing a pharmaceutical composition for alleviating IR, and has very broad application prospects.

The invention discloses an application of gelatinized intestinal epithelial cells in preparation of drugs for treating inflammatory bowel disease, the gelatinized intestinal epithelial cells take mouse intestinal epithelial cells as cores, poly (ethylene glycol) diacrylate, a photoinitiator and poly-L-lysine permeate into the cells through low-temperature freeze thawing, and an intracellular porous hydrogel skeleton is formed through ultraviolet crosslinking. The gelated intestinal epithelial cell has a strong cfDNA and inflammatory factor removal function, has good biocompatibility, has no direct or indirect toxic effect on a human body, has no potential toxicity, and can be used for treating inflammatory bowel diseases.

The mouse intestine roll specimen preparation device comprises a box body, a box cover, a sliding plate and an insertion column, the top face of the box body is open, and the box cover is connected to the opening of the top face of the box body in an inserted mode; the sliding plate is located on the inner bottom face of the box body, the side face of the sliding plate is slidably connected with the inner side wall of the box body, and the sliding plate slides in the vertical direction. The upper surface of the sliding plate is symmetrically provided with two rows of jacks, each row of jacks comprises three jacks, the lower ends of the insertion columns are provided with insertion blocks, and the insertion blocks are connected with the holes in a pluggable mode. According to the mouse intestine dehydration device, after the mouse intestine is completely dehydrated, the sliding plate and the insertion columns are moved upwards to be completely away from formalin solution, and the insertion columns are pulled out of the insertion holes, so that the mouse intestine can be conveniently taken down from the insertion columns, and subsequent sample manufacturing is facilitated; the mouse intestine is in a specific shape, and after the mouse intestine is soaked in a formalin solution and dehydrated, the mouse intestine has a plurality of straight sections, so that the later processing and manufacturing of specimens are facilitated.

The present invention provides a method for preparing a mouse intestinal tissue wax block for slicing, which comprises the following steps: sampling of intestinal tissue, fixation of intestinal tissue, dehydration of intestinal tissue, and embedding of intestinal tissue. The present invention has simple steps and convenient operation. The embedded intestinal tissue wax block can keep the intestinal tissue morphology intact during subsequent slicing, without or only occasionally showing muscle layer separation phenomenon; the connective tissue morphology of the submucosal layer is completely presented, which is convenient for scientific researchers to conduct comprehensive observation of specimens, and provides reliable materials for theoretical research on intestinal diseases.

The present application relates to the field of microbial sampling and detection, and particularly relates to a mouse experimental E. coli sampling and detection device. The technical problem to be solved is that when sampling and detecting E. coli in the intestinal tract of a mouse, the attention of manual operation needs to be highly concentrated, and it is easy to cause physical and mental fatigue, and the intestinal tract will be damaged if a little carelessness. In addition, the existing technology cannot remove the fat tissue existing on the outer surface of the intestinal tract, thereby affecting the sampling and detection result. The technical scheme is as follows: a mouse experimental E. coli sampling and detection device, comprising a sampling box and a fixing assembly. The first clamp block and the second clamp block are respectively arranged on the left end and the right end of the intestinal tract of the mouse to fix the intestinal tract, the injection tube is used to inject sterile water into the inside of the intestinal tract to flush, the cleaning of the intestinal tract of the mouse is completed, the pressure of manual operation is reduced, and the accidental damage of the intestinal tract of the mouse caused by manual operation is reduced.