Application of green seedling extract
A technology of extracts and green shoots, applied in the direction of food science, etc., can solve problems such as hidden health risks and large side effects, and achieve obvious protective effects
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Embodiment 1
[0055] 1. Cultivation: The seed of Job's tears is sterilized with 2% NaClO solution for 10 minutes, and washed 3 times with distilled water. Then disinfect with 70% ethanol for 30 seconds, rinse with distilled water three times, then add warm water (25°C), and soak for 12 hours at room temperature. Place in an artificial climate chamber for seed germination. The culture conditions are constant temperature day and night (25±1° C.), the light time is 12 hours, the light intensity is 3500 lx, and the humidity is 85%. During seed germination, the seeds need to be kept moist. Select young seedlings without joints, rich in flesh, and dark green in color. The growth period is 18 days, and they grow to a height of 20-30 cm. They are cut at 2.5-3 cm from the ground, and yellow leaves and impurities are removed for experiments.
[0056] 2. Extraction: extract barley seedlings in 60% ethanol at a solid-to-liquid ratio of 1:30 (g / mL), shake in a 70°C water bath for 120 minutes (150 rpm). ...
Embodiment 2
[0061] 1. Cultivation: The highland barley seeds were sterilized with 2% NaClO solution for 10 minutes, and washed 3 times with distilled water. Then disinfect with 70% ethanol for 30 seconds, rinse with distilled water three times, then add warm water (25°C), and soak for 12 hours at room temperature. Place in artificial climate chamber to carry out seed germination test, culture condition is constant temperature day and night (25 ± 1 ℃), light time is 12h, illuminance is 3500lx, humidity 85%, need keep seed moist during seed germination. Select young seedlings that are not jointed, rich in flesh, and dark green in color. The growth cycle is 15 days, and they grow to a height of 20-30 cm. They are cut at 2.5-3 cm from the ground, and yellow leaves and impurities are removed for experiments.
[0062] 2. Extraction: extract highland barley seedlings in 60% ethanol at a solid-to-liquid ratio of 1:30 (g / mL), shake in a 70°C water bath for 120 minutes (150 rpm).
[0063] 3. Filtr...
Embodiment 3
[0067] 1. Cultivation: Naked oat seeds are sterilized with 2% NaClO solution for 10 minutes, and rinsed with distilled water 3 times. Then disinfect with 70% ethanol for 30 seconds, rinse with distilled water three times, then add warm water (25°C), and soak for 12 hours at room temperature. Place in artificial climate chamber to carry out seed germination test, culture condition is constant temperature day and night (25 ℃ ± 1), light time is 12h, illuminance is 3500lx, humidity 85%, need keep seed moist during seed germination. Select young seedlings that are not jointed, rich in flesh, and dark green in color. The growth period is 19 days, and they grow to a height of 20-30 cm. They are cut at 2.5-3 cm from the ground, and yellow leaves and impurities are removed for experiments.
[0068] 2. Extraction: take naked oat seedlings and extract them in 60% ethanol at a material-to-liquid ratio of 1:30 (g / mL), shake in a 70°C water bath for 120 minutes (150 rpm).
[0069] 3. Filt...
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