Breeding method of glyphosate-resistant cotton genic male sterility dual line
A glyphosate-resistant and nuclear-sterile technology, which is applied in the directions of botanical equipment and methods, applications, and plant genetic improvement, can solve the problem of increased seed production cost of nuclear sterile dual-purpose hybrid cotton, affecting the normal growth of cotton, Seed production labor reduction and other issues, to achieve the effect of reducing manual weeding, reducing labor input and saving labor
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[0034] (1) In the summer of 2013, the GMS line GA18 and the glyphosate-resistant line GGK-2 (introduced from the Institute of Biotechnology, Chinese Academy of Agricultural Sciences) were planted in Chengdu. Before cross-pollination, GGK-2 grew to 6 true leaves and sprayed a 0.2% glyphosate solution on its growth points. After 9 days, all cotton seedlings grew normally, and the growth points did not wilt or Death, indicating that GGK-2 is 100% resistant to glyphosate. The fertility of GA18 was identified at the bud stage, and the sterile plants were marked. After flowering, use the flowers of GGK-2 to pollinate and cross the flowers on the GA18 sterile plant, harvest the hybrid boll seeds, and obtain the glyphosate-resistant genic male sterile hybrid F 1 (Material name RH).
[0035] (2) In the winter of 2013, hybrid RH and GMS line GA18 were planted in Hainan, and their resistance to glyphosate was identified when RH grew to 6 true leaves fully expanded (the method was the s...
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