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Breeding method of glyphosate-resistant cotton genic male sterility dual line

A glyphosate-resistant and nuclear-sterile technology, which is applied in the directions of botanical equipment and methods, applications, and plant genetic improvement, can solve the problem of increased seed production cost of nuclear sterile dual-purpose hybrid cotton, affecting the normal growth of cotton, Seed production labor reduction and other issues, to achieve the effect of reducing manual weeding, reducing labor input and saving labor

Active Publication Date: 2021-04-09
四川省农业科学院经济作物育种栽培研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with artificial emasculated hybrid cotton, the seed production of genie male sterile hybrid cotton does not require manual emasculation, the seed production procedure is simplified, the labor required for seed production is greatly reduced, and the efficiency is greatly improved. It is a hotspot in the research of hybrid cotton, but at the same time, the genetic male sterile hybrid cotton still needs manual weeding during planting, especially most cotton is not tolerant to glyphosate, and the normal growth of cotton will be affected after spraying glyphosate
With the continuous increase of labor price in our country, the seed production cost of GMS dual-purpose line hybrid cotton has increased significantly

Method used

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  • Breeding method of glyphosate-resistant cotton genic male sterility dual line
  • Breeding method of glyphosate-resistant cotton genic male sterility dual line
  • Breeding method of glyphosate-resistant cotton genic male sterility dual line

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Embodiment 1

[0034] (1) In the summer of 2013, the GMS line GA18 and the glyphosate-resistant line GGK-2 (introduced from the Institute of Biotechnology, Chinese Academy of Agricultural Sciences) were planted in Chengdu. Before cross-pollination, GGK-2 grew to 6 true leaves and sprayed a 0.2% glyphosate solution on its growth points. After 9 days, all cotton seedlings grew normally, and the growth points did not wilt or Death, indicating that GGK-2 is 100% resistant to glyphosate. The fertility of GA18 was identified at the bud stage, and the sterile plants were marked. After flowering, use the flowers of GGK-2 to pollinate and cross the flowers on the GA18 sterile plant, harvest the hybrid boll seeds, and obtain the glyphosate-resistant genic male sterile hybrid F 1 (Material name RH).

[0035] (2) In the winter of 2013, hybrid RH and GMS line GA18 were planted in Hainan, and their resistance to glyphosate was identified when RH grew to 6 true leaves fully expanded (the method was the s...

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Abstract

The invention provides a breeding method of a glyphosate-resistant cotton genic male sterility dual line, and relates to the technical field of new crop variety breeding. A sterile plant in the cotton genic male sterility dual line is hybridized with a glyphosate-resistant line, the sterile plant of the genic male sterility dual line is used as a recurrent parent (female parent) and is continuously backcrossed with a glyphosate-resistant fertile plant (male parent) in a filial generation for six generations, the glyphosate-resistant sterile plant and the fertile plant are selected for hybrid propagation, the glyphosate-resistant fertile plant in the next generation of planting is selfed to harvest single plants, the plants are planted to form plant rows, all the plant rows with glyphosate resistance are selected for continuous three generations of plant cross, and the glyphosate-resistant cotton genic male sterility dual line is cultivated. The breeding method is reasonable in step and easy to operate, the bred glyphosate-resistant genic male sterile line is high in hereditary purity, the glyphosate-resistant genic male sterile line is used as the female parent for producing genic male sterile hybrids, glyphosate can be sprayed to a seed preparation field for weeding, and therefore labor investment is reduced, labor is saved, and the production cost of hybrid cotton is reduced.

Description

technical field [0001] The invention belongs to the technical field of breeding of new crop varieties, and in particular relates to a breeding method of glyphosate-resistant cotton male sterile dual-purpose lines. Background technique [0002] As an important economic crop and strategic resource, cotton industry occupies an important position in the national economy. At present, the hybrid cotton varieties used in production are mainly artificial emasculated hybrid cotton, cytoplasmic sterile three-line hybrid cotton and nuclear sterile dual-purpose line hybrid cotton. Restricted the further development of hybrid cotton. [0003] The research and utilization of GMS hybrid cotton has continued since the 1970s, and it is one of the three hybrid cottons currently used in large-scale cotton production. Compared with artificial emasculated hybrid cotton, the seed production of genie male sterile hybrid cotton does not require manual emasculation, the seed production procedure i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 牟方生钟文娟周永航陈四维陈正杰戢沛城杨泽湖蒋理石盛佳宛永璐毛正轩王小强
Owner 四川省农业科学院经济作物育种栽培研究所
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