Method for culturing photosynthetic bacteria by utilizing membrane concentration biogas slurry

Abstract

The invention discloses a method for culturing photosynthetic bacteria by using membrane concentrated biogas slurry. The method comprises the following steps: detecting the content and pH value of nitrogen, phosphorus and potassium in the membrane concentration biogas slurry, diluting the concentrated biogas slurry according to the content of nitrogen, phosphorus and potassium, directly culturing photosynthetic bacteria by using the diluted membrane concentration biogas slurry as a culture solution, controlling the inoculation amount to be 10% of the volume of the culture solution, after inoculation, performing placing under the illumination intensity of 3000-5000Lux at the temperature of 30 DEG C, and performing culturing for 5-7 days. The membrane concentration biogas slurry is adopted as a culture solution of the photosynthetic bacteria, nutrient substances are richer after the biogas slurry is concentrated, nutrient substances do not need to be manually added any more, the dilution ratio of the concentrated biogas slurry is determined by detecting various indexes such as the nitrogen, phosphorus and potassium salt degree of the concentrated biogas slurry, and it is ensured that the concentrated biogas slurry meets the growth requirement of the photosynthetic bacteria. Most of microorganisms and all solid particles in the biogas slurry subjected to membrane concentration are removed.

Description

Technical field[0001]The present invention relates to the field of biotechnology, and more particularly to a method of culturing photosynthetic bacteria using a membrane.Background technique[0002]Photosynthetic bacteria is an prokaryotic organism that can utilize light energy as energy, all of which are Gram-negative bacteria, divided into oxygen-oxygen-based blue bacteria and non-oxygenated purple bacteria. Usually used in the purple bacteria in the purple bacteria, the swamp red psessasyl bacillus and capsular red psessa. Swamp red psessa primary is mainly used in low salinity water, which is mainly used in high salt water. Swamp red psessas used in the present invention. It not only performs non-oxygen-free light energy, but also can use organic substances to survive using organic matter in the case of oxygen in the dark. Widely existed in rivers, lakes, ponds, swamps and other places.[0003]Photosynthetic bacteria can utilize organics, ammonium nitrogen, hydrogen sulfide, nitrite...

AI Technical Summary

Problems solved by technology

However, the biogas slurry that has only been filtered is not only dark gray or dark brown in color and opaque, but also contains a small amount of solid particles and a large number of microorganisms, which obviously seriously affects the cultivation of photosynthetic bacteria.

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