Unlock instant, AI-driven research and patent intelligence for your innovation.

Cell culture method and cell culture apparatus

A cell culture and cell technology, used in tissue cell/virus culture devices, cell culture media, animal cells, etc., can solve the problems of inhibiting cell proliferation, reducing and increasing cell proliferation activity, and achieving low cost and mass culture. Effect

Pending Publication Date: 2021-04-09
NIKKISO COMPANY
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is known that when the cell density is continuously increased, the proliferation of the cells is inhibited
The reason for this is that the concentration of waste products (metabolites) in the culture solution (liquid medium) increases due to the high density of the cells, thereby reducing the growth activity of the cells.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cell culture method and cell culture apparatus
  • Cell culture method and cell culture apparatus
  • Cell culture method and cell culture apparatus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0037] The cell culture method of the present embodiment includes the step of culturing cells in a culture solution satisfying at least one of the following conditions (I) and (II).

[0038] Condition (I): the concentration of lactic acid in the culture solution is less than 7.5mM (7.5×10 －3 mol / L).

[0039] Condition (II): The ammonia concentration in the culture solution is less than 2.5 mM.

[0040] When lactic acid is contained in the culture medium, when the concentration of lactic acid is less than 7.5 mM, it is possible to suppress the inhibition of cell growth. In addition, it is also possible to suppress the inhibition of cell growth due to the decrease in the pH of the culture solution due to lactic acid. In addition, cells can be maintained in an undifferentiated state at concentrations of lactic acid less than 10 mM. Therefore, it is possible to reduce the influence of lactic acid on both the proliferating state and the undifferentiated state of the cells by cul...

Embodiment approach 2

[0068] Embodiment 2 has the same configuration as Embodiment 1 except that the structure of the cell culture apparatus is different. Hereinafter, the present embodiment will be described focusing on configurations different from those in Embodiment 1, and descriptions of common configurations will be briefly described or omitted. figure 2 It is the schematic diagram of the cell culture apparatus of Embodiment 2.

[0069] The cell culture device 1 of the present embodiment includes a culture container 2 and an adjustment unit 6 as main components. Cells 8 and a culture solution 10 are housed in the culture container 2 . The adjustment unit 6 adjusts the culture medium 10 so that at least one of the above-mentioned condition (I) and condition (II) is satisfied. The adjustment unit 6 of the present embodiment includes a storage container 12 , a supply pump 14 , a concentration sensor 4 , and a control unit 16 .

[0070] The storage container 12 stores a replenishment liquid 1...

Embodiment approach 3

[0073] Embodiment 3 has the same configuration as Embodiment 1 except that the structure of the cell culture apparatus is different. Hereinafter, the present embodiment will be described focusing on configurations different from those in Embodiment 1, and descriptions of common configurations will be simplified or omitted. image 3 It is the schematic diagram of the cell culture apparatus of Embodiment 3.

[0074] The cell culture device 1 of the present embodiment includes a culture container 2 and an adjustment unit 6 as main components. Cells 8 and a culture solution 10 are housed in the culture container 2 . The adjustment unit 6 adjusts the culture medium 10 so that at least one of the above-mentioned condition (I) and condition (II) is satisfied. The adjustment unit 6 of the present embodiment includes a substance exchanger 26 , a circulation pump 28 , a concentration sensor 4 , and a control unit 16 .

[0075] The material exchanger 26 accommodates the material excha...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

This cell culture method comprises a step for culturing cells 8 in a culture medium 10 that satisfies at least one among the following condition (I) and condition (II). Condition (I): the concentration of a lactic acid in the culture medium is less than 7.5 mM. Condition (II): the concentration of an ammonia in the culture medium is less than 2.5 mM.

Description

technical field [0001] The invention relates to a cell culture method and a cell culture device. Background technique [0002] In recent years, in fields such as pharmaceutical product manufacturing and regenerative medicine, it is necessary to artificially and efficiently culture large quantities of cells or microorganisms. Examples of cells requiring mass culture include antibody-producing cells such as Chinese hamster ovary cells (CHO cells), and pluripotent stem cells such as embryonic stem cells (ES cells) and artificial pluripotent stem cells (iPS cells). As long as these cells can be stably cultured in large quantities for a long period of time, biological substances such as monoclonal antibodies and differentiation-induced tissues derived from pluripotent stem cells can be efficiently produced. [0003] Suspension-stirring culture using a culture tank such as a spinner flask is conceivable as a method for industrially cultivating a large amount of cells. On the oth...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/074C12M3/00
CPCC12M41/32C12N5/0018C12N2500/30C12N2500/60C12M29/26C12M41/30C12M29/18C12M29/04C12M37/02C12M33/14C12N5/0607
Inventor 北川文彦
Owner NIKKISO COMPANY