Method for detecting high-density lipoprotein in serum
A high-density lipoprotein and serum technology, applied in the preparation of test samples, measuring devices, instruments, etc., can solve the problems of lack of HDL subclass analysis methods and lack of unified understanding, so as to improve efficiency, shorten electrophoresis time, Guaranteed accurate detection results
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Embodiment 1
[0031]A method of detecting high-density lipoprotein in serum, including the steps of:
[0032]Step 1, the sample preparation: 40 ul serum is prepared, and the centrifugation was carried out by the addition of an 8 ul phosphate tungsungnic acid solution, centrifugation was 8 min, the rotation speed was 2500 rpm, and the supernatant was placed in a new centrifuge tube;
[0033]Step 2, dyeing: 20 ul 0.1% (w / v) Sandan black b dyeing liquid and 10 ul40% sucrose solution were sequentially added in step 1, and mixed well and mixed.
[0034]Step 3, preparing an electrophoresis medium: The electrophoresis medium is used with a tubular gel system, and the upper layer employs a concentrated glue, the lower layer adopts a uniform concentration separation glue;
[0035]Step 4, electrophoresis: Put the electrophoresis medium into the disc-shaped electrophoresis instrument, add an electrophoretic buffer, and then slowly add the detection sample obtained by 35 ul step 2, first in electrophoresis at a curren...
Embodiment 2
[0050]A method of detecting high-density lipoprotein in serum, including the steps of:
[0051]Step 1, the sample preparation: 50 ul serum, centrifuged by adding 10 ul of phosphate tungsungstic acid solution for 10 min, centrifugal time is 10 min, the rotation speed is 2500 rpm, and the suction supernatant is placed in a new centrifuge tube;
[0052]Step 2, staining: 25 ul 0.5% (w / v) Sandan black B dyeing liquid and 15 ul40% sucrose solution were sequentially added in step 1, and mixed well and mixed.
[0053]Step 3, preparing an electrophoresis medium: The electrophoresis medium is used with a tubular gel system, and the upper layer employs a concentrated glue, the lower layer adopts a uniform concentration separation glue;
[0054]Step 4, electrophoresis: Put the electrophoretic medium into the disc-shaped electrophoresis instrument, add an electrophoretic buffer, and then slowly add the detection sample obtained by the 40 ul step 2, first in electrophoresis at a 2mA current, to be detected...
Embodiment 3
[0069]A method of detecting high-density lipoprotein in serum, including the steps of:
[0070]Step 1, the sample preparation: 60 ul serum is prepared, and the centrifugation is carried out by adding 12 ul phosphorite acid tungsionic acid solution, centrifugation is 12 min, the rotation speed is 2500 rpm, and the suction supernatant is placed in a new centrifuge tube;
[0071]Step 2, dyeing: 30 ul 0.1% (w / v) Sudanese black b dyeing liquid and 20 ul40% sucrose solution were sequentially added in step 1, and mixed well and mixed, and obtained the sample;
[0072]Step 3, preparing an electrophoresis medium: The electrophoresis medium is used with a tubular gel system, and the upper layer employs a concentrated glue, the lower layer adopts a uniform concentration separation glue;
[0073]Step 4, electrophoresis: Put the electrophoretic medium into the disc-shaped electrophoresis instrument, add an electrophoretic buffer, and then slowly add the detection sample obtained by 45 ul step 2, first in ...
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