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Method for extracting macromolecular collagen peptide through enzymolysis of fish scales

A collagen peptide and macromolecular technology, which is applied in the field of enzymatic hydrolysis of fish scales to extract macromolecular collagen peptides, and can solve the problem of easily producing a large amount of bitter peptides.

Pending Publication Date: 2021-04-20
海南华研胶原科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for extracting macromolecular collagen peptides by enzymatic hydrolysis of fish scales, so as to solve the problem that a large amount of bitter peptides is easily produced by the enzymatic extraction method

Method used

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Embodiment 1

[0023] A method for extracting macromolecular collagen peptides by enzymatic hydrolysis of fish scales, the method for extracting collagen peptides by enzymatic hydrolysis of fish scales is as follows:

[0024] The first step: pretreatment, through the cleaning of fish scales, including the first cleaning, the first filtration, the second cleaning, the second filtration, alkali cleaning, the third cleaning, the pickling, and the fourth cleaning;

[0025] The second step: substrate extraction, mix fish scales with appropriate amount of water, and heat, after continuous extraction for 60 minutes, lower the temperature to 50 degrees to obtain the substrate of fish scale extract;

[0026] The third step: enzymatic hydrolysis processing, using alkaline protease 2709 for enzymolysis processing, the temperature is controlled at 50 degrees, the amount of enzyme is 3%, and the reaction kettle is stirred to obtain A liquid;

[0027] The fourth step: to remove the bitter peptide, pour th...

Embodiment 2

[0039] A method for extracting macromolecular collagen peptides by enzymatic hydrolysis of fish scales, the method for extracting collagen peptides by enzymatic hydrolysis of fish scales is as follows:

[0040] The first step: pretreatment, through the cleaning of fish scales, including the first cleaning, the first filtration, the second cleaning, the second filtration, alkali cleaning, the third cleaning, the pickling, and the fourth cleaning;

[0041] The second step: substrate extraction, mix fish scales with appropriate amount of water, and heat, after continuous extraction for 60 minutes, lower the temperature to 50 degrees to obtain the substrate of fish scale extract;

[0042] The third step: enzymatic hydrolysis processing, using alkaline protease 2709 for enzymolysis processing, the temperature is controlled at 50 degrees, the amount of enzyme is 3%, and the reaction kettle is stirred to obtain A liquid;

[0043] The fourth step: to remove the bitter peptide, pour th...

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Abstract

The invention discloses a method for extracting macromolecular collagen peptide by fish scale enzymolysis. The method comprises the following steps of 1, performing pretreatment: cleaning fish scales, namely, performing primary cleaning, primary filtering, secondary cleaning, secondary filtering, alkaline cleaning, tertiary cleaning, acid cleaning and quartic cleaning; 2, performing substrate extraction: mixing the fish scales with a proper amount of water, performing heating, performing continuous extracting for 60 minutes, and performing cooling to 50 DEG C to obtain a substrate of a fish scale extracting solution; and 3, performing enzymolysis processing: selecting alkaline protease 2709 for enzymolysis processing, controlling the temperature to be 50 DEG C and the enzyme amount to be 3%, and performing stirring through a reaction kettle to obtain a solution A. The macromolecular collagen peptide can be obtained in an enzymolysis extraction mode, and bitter peptide generated during enzymolysis can be taken out in an activated carbon adsorption mode, so that edible products made of the collagen peptide are better in taste.

Description

technical field [0001] The invention belongs to the technical field of enzymatic hydrolysis of fish scales, in particular to a method for enzymatic hydrolysis of fish scales to extract macromolecular collagen peptides. Background technique [0002] The national freshwater fish production is about 6 million tons, and the waste accounts for four layers. In recent years, in addition to food processing, cephalin has been extracted from visceral oil, bones, etc., as well as fish skin tanning and fish scale extraction. [0003] Fish scale extraction can obtain high-protein, low-fat, and multi-trace element substances. Collagen peptide is one of them. After being fully absorbed and utilized by the human body, it will synthesize skin collagen and play a certain role in beauty. [0004] At present, when preparing collagen peptides, most of them use enzyme extraction method. Although the extraction rate is high, considering the amount of enzyme input, it is easy to produce a large amo...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/22C07K1/34
Inventor 李立郭红星周尽学
Owner 海南华研胶原科技股份有限公司