Method for identifying transgenic soybean plants through flower color

A technology of transgenic plants and flower colors, applied in botany equipment and methods, genetic engineering, plant genetic improvement, etc., can solve the problems of destroying tissue activity and not being easy to observe

Inactive Publication Date: 2021-04-27
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

GFP and DsRed2 are not easily visualized in soybean, and GUS staining destroys tissue viability

Method used

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  • Method for identifying transgenic soybean plants through flower color
  • Method for identifying transgenic soybean plants through flower color

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Experimental program
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Embodiment 1

[0050] Embodiment 1. A method for identifying transgenic soybean plants by flower color

[0051] 1. Cloning of GmZI gene

[0052] RNA was extracted from the flower of the purple-flowered soybean cultivar Zhonghuang42, and cDNA was synthesized by reverse transcription.

[0053] The cDNA was used as a template, and the primer pair composed of the new primer F and primer R was used for PCR amplification, and the product size was about 1500bp.

[0054] F: 5'-ATGGACTCATTGTTACTTCT-3';

[0055] R: 5'-CAACCAATTCTAAGAAATGTAA-3'.

[0056] After sequencing, the nucleotide sequence of the PCR product is sequence 1 in the sequence table, which is the ZI gene.

[0057] Two, the construction of GmZI overexpression vector

[0058] 1. The PCR product obtained in the above one is used as a template, and a primer pair consisting of primer F-inf and primer R-inf is used for PCR amplification, and a PCR amplification product of about 1500 bp is recovered.

[0059] F-inf: 5'-GAGAACACGGGGGACTCT...

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Abstract

The invention discloses a method for identifying transgenic soybean plants through a flower color. The invention provides a nucleic acid molecule. The nucleic acid molecule is a DNA molecule composed of a nucleotide sequence shown as a sequence 1 in a sequence table. A inventor of the invention finds that the soybean flower color is a phenotype which is easy to identify, a white flower can be changed into a purple flower through overexpression of a Z1 gene, and the method can be used as a visual phenotype identification index for identifying a soybean transgenic positive plant. Therefore, when placed on a visual report system, transgenic soybean plants can be detected conveniently and effectively.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for identifying transgenic soybean plants by flower color. Background technique [0002] At present, soybean genetic transformation mainly adopts the soybean cotyledon node method mediated by Agrobacterium tumefaciens. Most of the obtained T0 transgenic plants are chimeras, and homozygous transgenic plants are obtained at least from the T1 generation. A large number of transgenic plant identification work is in the T1 generation, and the detection is time-consuming, laborious and costly. Currently commonly used reporter genes include green fluorescent protein gene (GFP), β-glucuronidase gene (GUS), red fluorescent protein gene (DsRed2), etc., which are not suitable for soybean. GFP and DsRed2 are not easily visualized in soybean, and GUS staining will destroy tissue viability. An efficient and visualized transgenic plant screening system will have a major impact on soybean t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82C12N5/02A01H6/54G01N21/78
CPCC07K14/415C12N15/827G01N21/78
Inventor 侯文胜陈莉袁珊孙石
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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