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Corneal tissue culture system and corneal tissue culture method thereof

A tissue culture and cornea technology, applied in tissue culture, tissue cell/virus culture device, 3D culture, etc., can solve the problems of difficult operation, cell loss, corneal tissue edema, etc.

Pending Publication Date: 2021-05-07
CHUNG GUNG MEDICAL FOUNDATION LINKOU BRANCH
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The endothelial cell density of the corneal tissue is an important condition for the corneal tissue to maintain its physiological function. If the endothelial cell layer of the corneal tissue is damaged, the endothelial cell density of the corneal tissue decreases or the function is abnormal, it will cause edema of the corneal tissue, making it difficult to operate during the operation. In severe cases, it will cause the corneal tissue to be unusable. However, the known technology mainly only considers how to preserve the donor corneal tissue, but lacks attention on how to promote the proliferation of the endothelial cell layer of the corneal tissue. For example, the common corneal preservation solution is Optisol GS The disadvantage of preservation solution is that it requires higher cost, and the longer the storage time, the more cells will be lost

Method used

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  • Corneal tissue culture system and corneal tissue culture method thereof
  • Corneal tissue culture system and corneal tissue culture method thereof
  • Corneal tissue culture system and corneal tissue culture method thereof

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Embodiment Construction

[0066] [Statistical Analysis]

[0067] All numerical results are expressed as mean ± standard deviation. The comparison of data is done by using Microsoft Excel 2016 version for one-way ANOVA. The analysis of data adopts the p value of two-tailed test, where p<0.05* and p<0.01** indicates a significant difference.

[0068] [Corneal Tissue Culture System]

[0069] Please refer to figure 2 The corneal tissue culture system 1000 shown in the first embodiment includes a tissue culture tank 100 and a tissue culture solution 300, wherein the tissue culture tank 100 includes a dish body 1 and a tube body 2, and the dish body The body 1 has a first accommodating space 11 and a first opening 12, the pipe body 2 is arranged in the first accommodating space 11, and has a second accommodating space 21 and a second opening 22, the second opening 22 and the first opening 12 face a first direction A. The tissue culture medium 300 includes DMEM basal medium, fetal bovine serum (FBS), gen...

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Abstract

A corneal tissue culture system and a corneal tissue culture method for increasing corneal endothelial cell density, healing of damaged corneal endothelial cell, and stimulating corneal endothelial cell proliferation are disclosed. The corneal tissue culture system comprises a tissue culture medium and a tissue culture dish to reduce metabolic pressure and reduce corneal tissue edema.

Description

technical field [0001] The present invention provides a corneal tissue culture system for improving the quality of eye bank donor corneal tissue, especially a system for increasing the density of endothelial cell layer of corneal tissue, healing of endothelial cell layer damage of corneal tissue and stimulating corneal tissue Methods of endothelial cell layer proliferation. Background technique [0002] If the corneal tissue lesions can be treated timely and accurately at the initial stage, the probability of complete cure is quite high. However, if the corneal tissue lesions are too serious without early treatment, corneal transplantation must be performed to control the disease. However, due to the influence of the current religious or traditional concepts, the amount of donated corneal tissue is scarce, and the amount of donated corneal tissue is far smaller than the demand for corneal tissue transplantation, so the amount of donation is not proportional to the demand. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/24C12N5/079
CPCC12M21/08C12M23/06C12N5/0621C12N2500/42C12N2513/00C12M23/10C12N2500/36A61K35/30C12N2500/30
Inventor 陈宏吉郑兆珉黄玠诚薛诒仁
Owner CHUNG GUNG MEDICAL FOUNDATION LINKOU BRANCH
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