A method for collecting and processing variable-wavelength data of a liquid chromatograph ultraviolet detector
A technology of data acquisition and processing and ultraviolet detectors, which is applied in the field of liquid chromatography detection, can solve the problems that there is no combined application of variable wavelength and gradient elution, so as to ensure baseline consistency and data rationality, ensure data integrity, The effect of reducing the impact
Active Publication Date: 2022-06-28
上海仪电分析仪器有限公司
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However, when the variable wavelength operation and the gradient elution function are combined, the baseline will change significantly due to the superposition of the variable wavelength and gradient conditions, which will affect the analysis. Therefore, there is no combined application of variable wavelength and gradient elution.
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[0031] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments. The objects, technical solutions and advantages of the present invention will become clearer from the following description. It should be noted that the described embodiments are preferred embodiments of the present invention, but not all embodiments.
[0032] combine figure 1 and as figure 2 As shown, the above-mentioned method for acquiring and processing variable wavelength data of an ultraviolet detector of a liquid chromatograph includes the following steps:
[0033] S1, start to pass through the fixed wavelength λ n Data acquisition at a higher sampling frequency as the initial wavelength;
[0034] S2, perform t in step S1 n After -x duration, the transmission mechanism controls the grating to start to rotate and switches to λ n with subsequent wavelength λ n+1 Alternately performed dual-wavelength acquisition mode at a low...
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The invention relates to a method for collecting and processing variable-wavelength data of an ultraviolet detector of a liquid chromatograph, and specifically refers to a method for collecting and processing data that can realize an optimized variable-wavelength function and can perform joint control and detection with a gradient elution program . It consists of 4 steps. The data collection and processing method of the present invention can effectively improve the baseline stability of the detector under specific conditions (variable wavelength), improve the detection efficiency, and can realize seamless connection of spectrograms in different wavelength bands. By updating the subsequent wavelength pre-acquisition part in the process of changing the wavelength, it is sequentially connected with the data of the previous wavelength, avoiding the distortion and sudden change of the baseline caused by real-time data collection and display, and ensuring the baseline of the data in each wavelength collection time period Consistency and data rationality make spectrogram data set in a unified baseline and coordinate range for easy processing and analysis, and the extension realizes the joint application of variable wavelength and gradient elution.
Description
technical field [0001] The invention relates to a variable wavelength data acquisition and processing method for an ultraviolet detector of a liquid chromatograph, in particular to a data acquisition and processing method capable of realizing an optimized variable wavelength function and capable of jointly controlling and running detection with a gradient elution program , which belongs to the technical field of liquid chromatography detection. Background technique [0002] UV-Vis spectrophotometry is a method for measuring the absorbance of substances in the wavelength range of 190-800nm for identification, impurity inspection and quantitative determination. By measuring the absorbance of the substance at different wavelengths, and drawing the relationship between the absorbance and the wavelength, the absorption spectrum of the substance to be measured is obtained. Ultraviolet-visible detector (UV-VIS) in liquid phase is an extended application, which can realize dual w...
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IPC IPC(8): G01N30/74G01N21/33G01N30/02G01N30/86
CPCG01N30/74G01N21/33G01N30/02G01N30/86
Inventor 张云龙陈浩飞李娟李涛
Owner 上海仪电分析仪器有限公司