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A kind of wheat ear number per unit area main effect qtl site and closely linked kasp primer and application

A unit area, wheat technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial determination/inspection, etc., can solve the problems of long breeding time, lack of understanding, etc., to save breeding costs, increase stability, reduce Effects of phenotyping efforts

Active Publication Date: 2022-05-20
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the molecular mechanism of its inheritance is not understood, and the breeding time is long

Method used

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  • A kind of wheat ear number per unit area main effect qtl site and closely linked kasp primer and application
  • A kind of wheat ear number per unit area main effect qtl site and closely linked kasp primer and application
  • A kind of wheat ear number per unit area main effect qtl site and closely linked kasp primer and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Acquisition of main effect QTL QSn.sau-2D.2 and KASP-AX-111151907 molecular marker for number of panicles per unit area

[0028] (1) F 1 Generation, F 1 F 2 A high-generation self-bred genetic population containing 371 lines was obtained through the single-seed method;

[0029] (2) Using the 55K SNP gene chip to construct Chuannong 18×T1208 containing 371 lines (F 11 -F 13 ) of the recombined inbred population to scan the genome and construct a linkage genetic map, which covers 21 chromosomes of wheat with a total length of 4192.62cM;

[0030] (3) Planted at the Qionglai Wheat Breeding Base of Sichuan Agricultural University (Qianjin Town, Qionglai City, Sichuan Province, 30°25′N, 103°28′E, 493.3m above sea level) during 2015-2016, 2016-2017, and 2017-2018. Random block design is adopted, the plot length is 2m, each row has 10 plants, and the row spacing is 0.25m. Each strain is planted in 4 rows and repeated three times. The cultivation management is the...

experiment example 3

[0042] Experimental example 3 Application of KASP-AX-111151907 molecular marker in Chuannong 18×T1208 high-generation self-bred population

[0043] The same method as above was used to randomly select 109 strains (including two parents) in the Chuannong 18×T1208 advanced self-bred population to extract DNA for fluorescent quantitative PCR genotyping, and the final result was the same as that of Chuannong 18 with the same fluorescent signal It is recorded as A genotype, and those with the same fluorescent signal as T1208 are recorded as B genotype. Finally, it can be seen that there are 47 strains with A genotype, and 62 strains with B genotype and A gene. The number of spikes per unit area of ​​genotype strains was significantly higher than that of genotype B strains, and the results are shown in Table 2 and Table 3.

[0044] Table 2 shows the genotyping of 109 strains in Chuannong 18×T1208 high-generation self-bred population

[0045]

[0046]

[0047]

[0048]

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Abstract

The invention discloses a major QTL locus for the number of spikes per unit area of ​​wheat and a closely linked KASP primer and its application. The major QTL locus QSn.sau-2D.2 is located on the 2D chromosome of wheat and in the Chinese Spring Reference Genome. The physical position on the site is between 82.2Mb‑95.9Mb, and the SNP molecular markers on both sides are AX‑110544009 and AX‑109283238, respectively. The present invention discloses for the first time a main effect QTL site QSn.sau‑2D.2 for controlling the number of spikes per unit area of ​​wheat, which is a new QTL site for controlling the number of spikes per unit area of ​​wheat located at the physical position 82.2Mb‑95.9Mb on the 2D chromosome. QTL, this QTL can significantly increase the number of spikes per unit area of ​​wheat, which is of high value for high-yield breeding of wheat.

Description

technical field [0001] The invention relates to a main effect QTL locus for the number of spikes per unit area of ​​wheat, in particular to a main effect QTL locus for the number of ears per unit area of ​​wheat, closely linked KASP primers and applications thereof. Background technique [0002] Wheat is one of the most important food crops in the world, "feeding" about one-third of the world's population, and provides about 20% of protein and calories in all human food. In addition, wheat is also the second largest food crop in my country, and the output of wheat is closely related to national food security. As one of the three factors of wheat yield, the number of spikes per unit area is directly related to wheat yield. Increasing the number of spikes per unit area of ​​wheat has always been an important breeding goal of wheat. [0003] Studies have shown that the number of ears per unit area of ​​wheat is controlled by polygenes, and is more susceptible to environmental ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2600/172C12Q2531/113C12Q2563/107
Inventor 任天恒范涛蒋庆李治谭飞泉
Owner SICHUAN AGRI UNIV