Immunofluorescence sealing liquid remover and application thereof

Abstract

The invention discloses an immunofluorescence sealing strip liquid removing agent and an application thereof and relates to the field of immunoassay and biotechnology application. The immunofluorescence sealing strip liquid removing agent comprises the following components by volume, 25-35 parts of isopropanol and 65-75 parts of double distilled water, and the application of the immunofluorescence sealing strip liquid removing agent is characterized by comprising the following steps of A1, dropwise adding the remover to the periphery of a cover glass on a tissue slice, and removing the cover glass; A2, cleaning the tissue slice for 2-3 times at room temperature by using the remover, wherein the cleaning time is 5 minutes each time; and A3, cleaning the tissue slice once with a PBS solution for 5 minutes to obtain the tissue slice without the immunofluorescence slice sealing liquid. According to the method, after the sealing liquid is removed, the tissue slice or the cell sample is operated again, so the error-tolerant rate of an immunofluorescence experiment is increased, the number of samples is reduced, and the experiment time is saved.

Description

technical field [0001] The invention relates to the application fields of immunoanalysis and biotechnology, in particular to an immunofluorescence mounting fluid remover and application thereof. Background technique [0002] Immunofluorescence is often one-time, that is, it is observed with a fluorescence microscope or confocal laser immediately after an experiment. If the experimental samples are precious, for example, tissue samples of rare diseases or experimental samples that require long-term modeling, there are often no spare samples to make mistakes. At the same time, for precious samples, I want to use immunofluorescence to detect multiple indicators, but the existing immunofluorescence technology can only detect five colors at most, and is limited by the number of detection channels of the detection instrument, which leads to the required sample size Increase and extend the experiment time. Contents of the invention [0003] Aiming at the problems existing in th...

AI Technical Summary

Problems solved by technology

At the same time, for precious samples, I want to use immunofluorescence to detect multiple indicators, but the existing immunofluorescence technology can only detect five colors at most, and is limited by the number of detection channels of the detection instrument, which leads to the required sample size Increase, prolong the experiment time

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