Method for eliminating theophylline interference in immunoassay and immunoassay kit

An immunoassay and interfering agent technology, applied in the field of immunoassay, can solve problems affecting clinical judgment and interfering with the accuracy of immunoassay results, and achieve reliable judgment basis and simple method

Active Publication Date: 2022-07-12
武汉全景生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In clinical immunoassays, if there is a certain concentration of drugs or their metabolites in the subject's blood, it will interfere with the accuracy of the immunoassay results, and thus affect clinical judgment

Method used

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  • Method for eliminating theophylline interference in immunoassay and immunoassay kit
  • Method for eliminating theophylline interference in immunoassay and immunoassay kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] In this Example 1, the anti-theophylline-interfering ability of the above-mentioned anti-theophylline-interfering agents was evaluated.

[0059] First, prepare two sets of serum samples with the concentrations of precursor gastrin-releasing peptide (ProGRP) at (40.00±10.00) pg / mL and (600.00±120.00) pg / mL, two for each group; Add a certain volume of high-concentration theophylline solution (dissolved in absolute ethanol, and the added volume does not exceed 1 / 20 of the total volume after addition) to prepare an interference sample with theophylline concentration of 50 μg / mL; add to another sample An equal volume of absolute ethanol (the added volume is the same as the added volume of the aforementioned interference sample, and does not exceed 1 / 20 of the total volume after addition), to prepare a control sample without interference.

[0060] Here, the interfering samples and the control samples were tested twice using the gastrin-releasing peptide precursor chemilumines...

Embodiment 2

[0076] In this Example 2, the anti-theophylline-interfering ability of different final concentrations of anti-theophylline-interfering agents was evaluated in substantially the same manner as in Example 1.

[0077] In this example, the final concentrations of 1 μg / mL, 0.1 mg / mL, 1 mg / mL, 5 mg / mL, 10 mg / mL, 20mg / mL, 50mg / mL, 80mg / mL, 100mg / mL of purine, and then according to the same method as in Example 1, the concentrations of precursor gastrin-releasing peptide (ProGRP) were (40.00±10.00) pg / mL, (600.00±120.00) pg / mL serum samples containing 50μg / mL theophylline interference or without any interference were measured, and the interference deviation B was calculated. The results are shown in the following table.

[0078]

[0079] It can be seen from the results in the above table that different final concentrations of purine can reduce or even eliminate the interference caused by theophylline in immunoassays to varying degrees. As the concentration of purine in the gastrin...

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Abstract

A method for eliminating theophylline interference in immunoassay and an immunoassay kit. The method for eliminating theophylline interference in the immunoassay comprises: performing an immunoassay reaction in the presence of an anti-theophylline interfering agent, wherein the final concentration of the anti-theophylline interfering agent is 0.1 mg/mL to 100 mg/mL, and is selected from 6-mercapto At least one of the group consisting of purine, 6-mercaptoguanine, 8-azaguanine, hypoxanthine, adenine, purine, 2,6,8-trioxopurine, adenine nucleotide and adenosine kind. The method and the kit can effectively reduce the interference of theophylline on the results of immunoassays.

Description

technical field [0001] The invention relates to the field of immune detection, in particular to a detection method for eliminating the influence of theophylline drugs on immune detection for patients taking theophylline drugs. Background technique [0002] Theophylline is a class of anti-asthmatic drugs that have a direct relaxing effect on the smooth muscle of the respiratory tract. Theophylline drugs are suitable for the treatment of asthma (such as bronchial asthma), chronic bronchitis, wheezing bronchitis, emphysema, chronic obstructive pneumonia and other diseases, and play a role in relaxing the lung airways and increasing airflow, thereby relieving wheezing symptom. Theophylline is also used to treat wheezing in heart failure. [0003] For a certain period of time after taking theophylline drugs, the patient's blood will contain a certain amount of the drug. In clinical immunoassays, if there is a certain concentration of drugs or their metabolites in the blood of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53
CPCG01N33/5306G01N33/54387G01N33/57484G01N33/57488
Inventor 李可詹成雄张裕平
Owner 武汉全景生物技术有限公司
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