Hepatocyte large-scale culturing and freezing device

Abstract

The invention relates to the technical field of cell culture, and discloses a hepatocyte large-scale culturing and freezing device. The large-scale hepatocyte culture and freezing device comprises a base, a box body, a placement frame, a refrigerator, a power assembly and an air passing assembly, the top end of the base is fixedly connected with the box body, the left side of the inner wall of the box body is fixedly connected with the placement frame, and the right side of the inner wall of the box body is fixedly connected with the air passing assembly; the right side of the air passing assembly is fixedly connected with the refrigerator, and the front face of the box body is movably connected with the power assembly. A transmission block drives a second transmission belt to rotate through a transmission shaft and a first transmission belt, rotating force of a first knob is slowly transmitted to the second transmission belt, a rotating ring drives a rolling rod to rotate, the rolling rod drives a rolling wheel to rotate, and the rolling wheel exerts pushing force on a baffle plate through a wedge-shaped groove, so that the baffle plate is slowly away from an air passing pipe, and the drift diameter of the air passing pipe is gradually increased; the temperature in the box body is not suddenly reduced, so that the hepatocytes are prevented from generating relatively large ice crystals, and the survival rate of the hepatocytes is improved.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a device for large-scale culture and freezing of hepatocytes. Background technique [0002] Hepatocytes are highly differentiated cells, which are the parenchymal cells of the liver and the most important cells that make up the liver, accounting for 80% of the volume and number of the liver. Cell culture, this culture method can no longer keep up with the demand for liver cells in modern society. The principle of slow freezing and freezing of cells must be implemented when freezing cells. Large ice crystals, the existing large-scale hepatocyte culture device has some disadvantages. Due to the large scale of the culture device, it is difficult to control the temperature when freezing the cells. If the cells are not frozen slowly, the cells will These large ice crystals will cause damage and rupture of cell membranes and organelles. During the cell recovery stage, the cells s...

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Problems solved by technology

[0002] Hepatocytes are highly differentiated cells, which are the parenchymal cells of the liver and the most important cells that make up the liver, accounting for 80% of the volume and number of the liver. Cell culture, this culture method can no longer keep up with the demand for liver cells in modern society. The principle of slow freezing and freezing of cells must be implemented when freezing cells. Large ice crystals, the existing large-scale hepatocyte culture device has some disadvantages. Due to the large scale of the culture device, it is difficult to control the temperature when freezing the cells. If the cells are not frozen slowly, the cells will These large ice crystals will cause damage and rupture of cell membranes and organelles. During the cell recovery stage, the cells should be melted quickly to prevent the original small ice crystals from forming large ice crystals, that is, heavy ice crystals. crystallization, therefore, we propose a device for slowly cooling large-scale culture and freezing of hepatocytes

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