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Bacillus albus J1 strain and application thereof

The technology of a white bacillus and strains is applied in the directions of bacteria, microorganisms, buildings, etc., which can solve the problems such as no disclosure of the white bacillus, waste of calcium sources, no involvement of the mineralization of the white bacillus, etc., and has a large industrial application prospect and is easy to form. The effect of commercialization and convenient transportation

Active Publication Date: 2021-05-25
CHENGDU UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Practical industrial application will cause great waste of calcium source
[0004] The prior art has not dealt with the mineralization of Leucobacter sp. microorganisms nor disclosed any Leucobacter sp. strains capable of mineralization

Method used

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  • Bacillus albus J1 strain and application thereof
  • Bacillus albus J1 strain and application thereof
  • Bacillus albus J1 strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Strain screening and identification.

[0031] 1. Strain screening

[0032] 1.1 Samples, media, solutions

[0033] Solid samples of travertine: The surface of travertine deposits in Jiuzhaigou Natural Scenic Area, Jiuzhaigou County, Aba Tibetan and Qiang Autonomous Prefecture, Sichuan Province was collected with a sterile scraper.

[0034] B4 solid medium: calcium acetate 2.5g / L, glucose 5g / L, yeast powder 4.0g / L, agar 15.0g / L, pH 8.0.

[0035] B4 liquid medium: calcium acetate 2.5g / L, glucose 5g / L, yeast powder 4.0g / L, pH value 8.0.

[0036] LB solid medium: sodium chloride 10g / L, yeast powder 5g / L, peptone 10g / L, pH 7.

[0037] All media were sterilized by high temperature and high pressure before use.

[0038] Dilute hydrochloric acid: 1mol / L.

[0039] 1.2 Isolation and purification of bacterial strains

[0040] 1.2.1 Initial screening

[0041] Weigh 5g of the travertine solid sample in an ultra-clean aseptic operating bench, add 5ml of sterile water to shake e...

Embodiment 2

[0062] The CGMCC No. 21284 of the white bacillus J1 strain of the present invention is applied to the calcium mineralization / calcium precipitation reaction.

[0063] 1. Medium

[0064] B4 liquid culture medium, with embodiment one.

[0065] LB liquid medium: peptone 10g / L, yeast powder 5g / L, sodium chloride 10g / L, pH 7.0.

[0066] All media were sterilized by high temperature and high pressure before use.

[0067] 2. Experimental method

[0068] 2.1 Calcium mineralization culture

[0069] The purified J1 strain was cultured in LB liquid medium to logarithmic phase (OD600=1.0). Draw 1ml of inoculum into a conical flask filled with 100mL of B4 liquid medium on a sterile operating table, culture at 30°C and shake at a constant temperature of 120r / min. After culturing for 28 days, the culture medium of the strain was obtained. Set up three parallel experimental groups.

[0070] Set 100mL B4 liquid medium without inoculation as the control group. The control group was cultu...

Embodiment 3

[0082] Experiments on optimum growth temperature and optimum growth pH conditions of CGMCC No. 21284 strain CGMCC No.21284 of the present invention.

[0083] 1. Medium

[0084] LB liquid culture medium is the same as embodiment two.

[0085] All media were sterilized by high temperature and high pressure before use.

[0086] 2. Experimental methods and results

[0087] 2.1 Determination of the optimum growth temperature of J1 strain

[0088] Cultivate the purified J1 strain to the logarithmic phase (OD600=1.0) in LB liquid medium, draw 1ml of the logarithmic phase bacterial liquid on the aseptic operation bench and inoculate it into a conical flask equipped with 100mL LB liquid medium, They were cultured at 10°C, 20°C, 25°C, 30°C, 35°C, and 40°C, respectively. The static culture was finished for 24 hours. The experiment was repeated for 3 groups. After the culture is finished, samples are taken from the culture, and the concentration of bacteria in the samples is detecte...

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Abstract

The invention discloses a Bacillus albus J1 strain and application thereof. The bacillus albus J1 strain CGMCC No.21284 is screened from a travertine solid sample in a natural scenic area of Juzhaigou Valley County of Juzhaigou County of Tibetan Qiang Autonomous Pzhou in Sichuan, and can mineralize calcium in a culture medium and convert calcium ions in the culture medium into calcium carbonate. The J1 strain is cultured for 28 days under laboratory conditions, the calcium mineralization / calcium precipitation efficiency of the J1 strain is as high as 97% on average, and the industrial application prospect is wide. Calcium mineralization / calcium precipitation culture conditions suitable for the J1 strain are as follows: the pH is 6-7 and the temperature is 20-35 DEG C. A calcium mineralization / calcium precipitation reaction of the J1 strain has no by-product, is highly environment-friendly and safe, and is obviously superior to the existing common microorganisms with calcium precipitation purpose. The invention simultaneously provides multiple purposes of the Bacillus albus J1 strain and a calcium precipitation product taking the Bacillus albus J1 strain as a utility component.

Description

technical field [0001] The invention relates to a microorganism belonging to the genus Albus, in particular to a calcium mineralizing / calcifying bacteria J1 strain, which belongs to the technical field of industrial microorganisms. Background technique [0002] On the surface of the earth, microorganisms that have a major impact on the formation of carbonate rocks mainly include bacteria, fungi, algae and multicellular organisms, and most of the minerals formed are precipitated as calcium carbonate. This phenomenon is called Microbial induced carbonate precipitation (MICP) or Microbial induced mineralization. The use of microorganisms to induce the mineralization of calcium ions in the environment to precipitate into calcium carbonate has a very wide range of practical or potential applications. Including the restoration of immobilized divalent heavy metals and radionuclides, the restoration of limestone cultural relics, and the performance improvement of geotechnical engin...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P3/00E02D3/00C12R1/01
CPCC12P3/00E02D3/00
Inventor 谢鸿观杨明浩陈聪裴向军雷泞菲杜杰童晋
Owner CHENGDU UNIVERSITY OF TECHNOLOGY
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