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Alcaligenes aquatilis DF-27 strain and application thereof

A DF-27, a technology for producing alkali bacteria, applied in the direction of bacteria, microorganisms, microorganisms, etc., can solve the problems of industrial use in the field of biological deodorization/deodorization technology that have not yet been found, and achieve convenient transportation, quick start, and high deodorization efficiency. Effect

Active Publication Date: 2019-08-30
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prior art has not yet found its industrial use in the field of biological deodorization / deodorization technology

Method used

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  • Alcaligenes aquatilis DF-27 strain and application thereof
  • Alcaligenes aquatilis DF-27 strain and application thereof
  • Alcaligenes aquatilis DF-27 strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Strain screening and identification.

[0021] 1. Strain screening

[0022] 1.1 Sample and culture medium

[0023] Samples: Select 5 sampling points from the aerobic composting tank of Sichuan Zhongnong Runze Biotechnology Co., Ltd. (sampling points cover the compost heating stage, high temperature stage, and cooling stage), and use a sampler to collect 500g of compost material from each sampling point. Then the compost materials collected at 5 points were mixed, and then 500g was collected therefrom as a sample for subsequent use. The aerobic composting raw materials include various raw materials such as chicken manure, pig manure, sludge, sawdust, fungus residue and the like.

[0024] Enriched acclimatization medium: (NH 4 ) 2 SO 4 4.5g, sodium succinate 12.35g, glucose 4.5g, sodium acetate 4.5g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.1g, NaCl 0.2g, FeSO 4 ·7H 2 O 0.01g, MnSO 4 0.005g, add water to 1000ml, pH7.0. Sterilize by steam at 121℃ for 20min.

[0025]...

Embodiment 2

[0037] The Alcalibacterium water production DF-27 strain CGMCC No.16149 of the present invention is applied to the removal of nitrogen-containing malodorous substances.

[0038] 1. Medium

[0039] Growth and preservation medium: beef extract 3.0g, peptone 10.0g, NaCl 5.0g, H 2 O 1000ml, pH 7.0. Sterilize by steam at 121°C for 20min.

[0040] High-concentration ammonium ion medium: (NH 4 ) 2 SO 4 3.8g, sodium succinate 13.5g, glucose 6.0g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.1g, NaCl 0.2g, FeSO 4 ·7H 2 O 0.01g, MnSO 4 0.005g, add water to 1000ml, pH7.0. Dispense each 150ml into 250ml Erlenmeyer flasks, and sterilize with high-temperature steam at 121°C for 20min.

[0041] Low concentration ammonium ion medium: (NH 4 ) 2 SO 4 0.5g, sodium succinate 5.4g, glucose 1.6g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.1g, NaCl 0.2g, FeSO 4 ·7H 2 O 0.01g, MnSO 4 0.005g, add water to 1000ml, pH7.0. Dispense each 150ml into 250ml Erlenmeyer flasks, and sterilize with high-t...

Embodiment 3

[0051] The water-producing Alcalibacterium DF-27 strain CGMCC No.16149 of the present invention is applied to the removal of volatile acids.

[0052] 1. Medium

[0053] Growth and storage medium are the same as in Example 2.

[0054] Volatile acid removal experiment medium: 1.5ml formic acid, 1.5ml acetic acid, 1.5ml propionic acid, 1.5g glucose, (NH 4 ) 2 SO 4 1.0g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.1g, NaCl 0.2g, FeSO 4 ·7H 2 O 0.01g, MnSO 4 0.005g, add water to 1000ml, pH7.0. Dispense each 150ml into 250ml Erlenmeyer flasks, and sterilize with high-temperature steam at 121°C for 20min.

[0055] 2. Experimental method

[0056] The strain obtained in Example 1 was inoculated into a test tube containing 5 ml of growth and preservation medium, and cultured at 35° C. for 1 d under aerobic conditions. Observe that the bacterial liquid grows well, and the activated bacterial liquid is obtained.

[0057] Experimental group: Inoculate the activated bacterial liquid i...

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Abstract

The invention discloses alcaligenes aquatilis DF-27 strain and an application thereof. The strain is screened from trough compost and deposited in the China General Microbiological Culture CollectionCenter on July 25, 2018, with deposit number of CGMCC No. 16149. The strain can be used for biological deodorization and removing odorous substances in nitrogen-containing compounds and volatile acidsubstances in formic acids and / or acetic acids and / or propionic acids. The strain can also be applied in biological deodorization of dung compost, and realize in-situ deodorization for single or composite poultry excrement. The strain has instant start for deodorization, excellent effect, more than 99% of ammonium nitrogen removal rate and 96.46% of total removal rate to the concentration of threevolatile acid substances. The strain is screened from environmental samples stressed by ammonia, hydrogen sulfide, volatile acids, etc., for a long term, and complies with ecological safety regulations.

Description

technical field [0001] The invention relates to a water-producing alkali bacterium, in particular to a water-producing alkali bacterium that can be applied to biological deodorization and deodorization, and belongs to the technical field of industrial microorganisms. Background technique [0002] Odor is one of the seven major environmental hazards in the world. It is a hazard to the environment caused by the release of odorous gases from the complex reduction reaction of odorous substances under anaerobic conditions. Malodorous substances can be divided into five categories according to their composition: nitrogen-containing compounds, sulfur-containing compounds, oxygen-containing compounds, hydrocarbons, halogens and their derivatives. The pollution of bad volatile gases to the environment is caused by the release of gas molecules into the environment by certain volatile compounds that have been designated as odorous substances, causing discomfort and disgust to the human...

Claims

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Application Information

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IPC IPC(8): C12N1/20B01D53/84C12R1/05
CPCB01D53/84B01D2257/90C12N1/205C12R2001/05Y02A50/20
Inventor 袁月祥孙将李建章黄生田闫志英廖银章
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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