Alcalibacterium df-27 strain for water production and its application
A DF-27, a technology for the production of Alkaline bacteria, which is applied in the direction of bacteria, microorganisms, microorganisms, etc., can solve the problems of industrial applications in the field of biological deodorization/deodorization technology that have not yet been discovered, and achieve the effects of convenient transportation, quick start, and convenient use
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0020] Strain screening and identification.
[0021] 1. Strain screening
[0022] 1.1 Sample and culture medium
[0023] Samples: Select 5 sampling points from the aerobic composting tank of Sichuan Zhongnong Runze Biotechnology Co., Ltd. (sampling points cover the compost heating stage, high temperature stage, and cooling stage), and use a sampler to collect 500g of compost material from each sampling point. Then the compost materials collected at 5 points were mixed, and then 500g was collected therefrom as a sample for subsequent use. The aerobic composting raw materials include various raw materials such as chicken manure, pig manure, sludge, sawdust, fungus residue and the like.
[0024] Enriched acclimatization medium: (NH 4 ) 2 SO 4 4.5g, sodium succinate 12.35g, glucose 4.5g, sodium acetate 4.5g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.1g, NaCl 0.2g, FeSO 4 ·7H 2 O 0.01g, MnSO 4 0.005g, add water to 1000ml, pH7.0. Sterilize by steam at 121℃ for 20min.
[0025]...
Embodiment 2
[0037] The Alcalibacterium water production DF-27 strain CGMCC No.16149 of the present invention is applied to the removal of nitrogen-containing malodorous substances.
[0038] 1. Medium
[0039] Growth and preservation medium: beef extract 3.0g, peptone 10.0g, NaCl 5.0g, H 2 O 1000ml, pH 7.0. Sterilize by steam at 121°C for 20min.
[0040] High-concentration ammonium ion medium: (NH 4 ) 2 SO 4 3.8g, sodium succinate 13.5g, glucose 6.0g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.1g, NaCl 0.2g, FeSO 4 ·7H 2 O 0.01g, MnSO 4 0.005g, add water to 1000ml, pH7.0. Dispense each 150ml into 250ml Erlenmeyer flasks, and sterilize with high-temperature steam at 121°C for 20min.
[0041] Low concentration ammonium ion medium: (NH 4 ) 2 SO 4 0.5g, sodium succinate 5.4g, glucose 1.6g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.1g, NaCl 0.2g, FeSO 4 ·7H 2 O 0.01g, MnSO 4 0.005g, add water to 1000ml, pH7.0. Dispense each 150ml into 250ml Erlenmeyer flasks, and sterilize with high-t...
Embodiment 3
[0051] The water-producing Alcalibacterium DF-27 strain CGMCC No.16149 of the present invention is applied to the removal of volatile acids.
[0052] 1. Medium
[0053] Growth and storage medium are the same as in Example 2.
[0054] Volatile acid removal experiment medium: 1.5ml formic acid, 1.5ml acetic acid, 1.5ml propionic acid, 1.5g glucose, (NH 4 ) 2 SO 4 1.0g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.1g, NaCl 0.2g, FeSO 4 ·7H 2 O 0.01g, MnSO 4 0.005g, add water to 1000ml, pH7.0. Dispense each 150ml into 250ml Erlenmeyer flasks, and sterilize with high-temperature steam at 121°C for 20min.
[0055] 2. Experimental method
[0056] The strain obtained in Example 1 was inoculated into a test tube containing 5 ml of growth and preservation medium, and cultured at 35° C. for 1 d under aerobic conditions. Observe that the bacterial liquid grows well, and the activated bacterial liquid is obtained.
[0057] Experimental group: Inoculate the activated bacterial liquid i...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com