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Multiple fluorescence detection spectrum splitting method and device, equipment, medium and system

A multiple fluorescence and spectrum technology, applied in the field of fluorescence spectrum detection, can solve the problems of complex process and signal interference.

Active Publication Date: 2021-05-25
广州睿贝医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the overlapping wavelengths of the excitation light and emission light of various fluorescent dyes, and the limitation of optical hardware, there will be signal interference between different fluorescent dyes
[0003] In some scenarios, it is necessary to detect the fluorescence signal of a single fluorescent dye. For mixed multi-dye, if the fluorescent signal of a single fluorescent dye needs to be obtained, the mixed multi-dye needs to be purified, and then detected by excitation and emission , the process is relatively complicated

Method used

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  • Multiple fluorescence detection spectrum splitting method and device, equipment, medium and system
  • Multiple fluorescence detection spectrum splitting method and device, equipment, medium and system
  • Multiple fluorescence detection spectrum splitting method and device, equipment, medium and system

Examples

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Embodiment 1

[0053]Seefigure 1 ,figure 1 It is a flow chart of a spectral split method of a multi-fluorescence detection disclosed in the embodiment of the present invention. The execution body of the method described in the embodiments of the present invention can be applied to spectroscopy equipment, flow cytometry, multi-function microplate reader, fluorescence microscope, fluorescent quantitative PCR, etc. You can also use your mobile phone, tablet, server, etc. with devices with processing and storage functions. Such asfigure 1 As shown, the spectral split method of the multi-fluorescence detection includes the following steps:

[0054]110. The fluorescence intensity of each fluorescent passage collected, the fluorescence passage corresponding to the emission passage of the mixed multi-dye excitation.

[0055]Fluorescence intensity, spectral signal, and fluorescent signals, all refer to fluorescence information emitted by the emitting passage after the dye is excited. In some cases, it is shown t...

Embodiment 2

[0094]Seefigure 2 ,figure 2 It is a schematic structural diagram of a spectral split device (also known as a multi-dye mixing fluorescence detecting device) of the embodiment of the present invention. Such asfigure 2 As shown, the multi-fluorescent detection spectrum splitting device, which may include:

[0095]The receiving unit 210 is configured to receive the fluorescence intensity of each fluorescent passage acquisition, the fluorescence passage corresponding to the emission passage of the mixed multi-dye excitation;

[0096]The decomposing unit 220 is used to decompose fluorescence intensity of each fluorescent passage:

[0097]

[0098]Where CiFluorescence intensity, C of the i-th fluorescence passageI-Dyej It is the fluorescence intensity of the JIN of the JIN in the first fluorescent passage; 1 ≤ i ≤ N, 1 ≤ J ≤ M, M ≤ N, M is the total number of dyes, and n is the total number of fluorescent channels;

[0099]The selection unit 230 is used to select the fluorescence intensity of each of th...

Embodiment 3

[0106]Seeimage 3 ,image 3 A structural diagram of a spectral split system (also known as a multi-dye mixing fluorescence detection system) disclosed in the examples of the present invention. Such asimage 3 As shown, the multi-fluorescence detection spectrum split system can include:

[0107]The excitation mechanism 310 is used to excite the mixed multi-dye, produce a fluorescent signal, and transmit the fluorescent signal from the emission passage;

[0108]The conversion mechanism 320 is configured to receive the fluorescent signal of each transmit channel, and convert the fluorescent signal from the optical signal to the electrical signal of fluorescent intensity;

[0109]Electronic device 330, is used to perform some or all of the steps in the spectral split method of the multi-fluorescence detection in the first embodiment.

[0110]In a preferred embodiment of the invention, the excitation mechanism 310 can employ a single excitation source, or multiple excitation light sources can also be e...

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Abstract

The embodiment of the invention relates to the technical field of fluorescence spectrum detection, and discloses a multiple fluorescence detection spectrum splitting method and device, equipment, a medium and a system. The method comprises the steps: receiving collected fluorescence intensity of each fluorescence channel; decomposing the fluorescence intensity of each fluorescence channel; selecting the fluorescence intensity of each dye in one fluorescence channel as the reference fluorescence intensity of each dye, and determining the fluorescence intensity of each dye in each fluorescence channel by using the reference fluorescence intensity; calculating the reference fluorescence intensity of each dye according to the fluorescence intensity of the selected at least m fluorescence channels; and determining the fluorescence intensity of each dye in each fluorescence channel based on each reference fluorescence intensity. According to the embodiment of the invention, on the basis of measured values of multiple excitation and multiple emission channels, heterozygous spectral signals generated when different fluorescent dyes are simultaneously detected can be split into original spectra of single dyes; and single fluorescence signal resolution during joint detection of multiple fluorescent dyes is realized.

Description

Technical field[0001]The present invention relates to the field of fluorescence spectral detection, and more particularly to a spectral split method, apparatus, apparatus, medium, and system of multi-fluorescence detection.Background technique[0002]The fluorescent dye refers to a substance that absorbs a fluorescence after absorbing a wavelength of optical wave, which is widely used in modern biology, medicine, optical and other fields. However, due to the various fluorescent dyes excitation light, the wavelength of the emission light will overlap, and the optical hardware is limited, and there is signal interference between the different fluorescent dyes.[0003]In some scenes, it is necessary to detect a fluorescent signal of a single fluorescent dye. For mixed multi-dyed, if a fluorescent signal of a single fluorescent dye is needed, it is necessary to purify the mixed multi-dye, and then detect and transmit it by excitation and emission. The process is relatively complicated.Inven...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N2021/6421
Inventor 公杰张俊宾郭求真谭卿
Owner 广州睿贝医学科技有限公司
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