Immunoblotting instrument

Abstract

The invention discloses an immunoblotting instrument. Theimmunoblotting instrument comprises an immunoblotting instrument shell, and the immunoblotting instrument is divided into an incubation space, a reagent storage space and a control space; a touch display screen and a control mainboard are arranged at the upper part of the control space; an incubation groove is formed in the upper portion of the incubation space, an incubation assembly is movably arranged in the incubation groove, and a driving assembly used for driving the incubation assembly to vibrate is arranged on the lower portion of the incubation space; a reagent storage groove is formed in the reagent storage space, and an infusion catheter is arranged between the reagent storage groove and the incubation groove; and a refrigeration assembly is arranged on the lower portion of the incubation space and / or the control space and used for outputting cold energy to the incubation groove and the reagent storage groove. The immunoblotting instrument is more compact in structure and more reasonable in space layout, and meanwhile, the refrigeration assembly is additionally arranged, so the environment temperature for reagent storage and the environment temperature for incubation reaction can be adjusted and controlled, the activity of protein is guaranteed, parts of the immunoblotting instrument are reduced, and installation and maintenance are facilitated.

Description

technical field [0001] The invention relates to the field of western blot analysis, more specifically, it relates to a fully automatic western blot instrument. Background technique [0002] Western blotting is often used to identify a certain protein, and can perform qualitative and semi-quantitative analysis of the protein. It is widely used in the fields of life science research and laboratory medicine (such as tumor marker detection, pathogenic microorganism detection, etc.). In this method, the target protein separated by electrophoresis is transferred from the gel to a solid support (such as NC membrane or PVDF membrane), and then hybridized with a specific ligand of the known protein (such as an antibody, etc.), through an enzyme system After color development, the target protein is detected, and the immunodetection part includes blocking, primary antibody hybridization, washing, secondary antibody hybridization, and washing in five steps, involving multi-step liquid a...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of large volume, scattered and complex structure, and difficult installation and maintenance in the prior art, this application proposes a western blotting instrument, which uses a modular design idea to integrate various functions into different module structures In addition, the functional reuse of part of the module structure is realized, which makes the entire western blotting instrument compact in structure, effectively reduces the number of parts, and is very convenient for production, installation, use and maintenance. The specific scheme is as follows:

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