The invention discloses an immunoblotting instrument. Theimmunoblotting instrument comprises an immunoblotting instrument shell, and the immunoblotting instrument is divided into an incubation space, a
reagent storage space and a
control space; a touch display screen and a control mainboard are arranged at the upper part of the
control space; an incubation groove is formed in the upper portion of the incubation space, an incubation
assembly is movably arranged in the incubation groove, and a driving
assembly used for driving the incubation
assembly to vibrate is arranged on the lower portion of the incubation space; a
reagent storage groove is formed in the
reagent storage space, and an
infusion catheter is arranged between the reagent storage groove and the incubation groove; and a
refrigeration assembly is arranged on the lower portion of the incubation space and / or the
control space and used for outputting cold energy to the incubation groove and the reagent storage groove. The immunoblotting instrument is more compact in structure and more reasonable in space
layout, and meanwhile, the
refrigeration assembly is additionally arranged, so the environment temperature for reagent storage and the environment temperature for incubation reaction can be adjusted and controlled, the activity of
protein is guaranteed, parts of the immunoblotting instrument are reduced, and installation and maintenance are facilitated.