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Intraocular fluid cytokine detection reagent, detection method and application

A technology for detecting reagents and cytokines, which is applied in the field of intraocular fluid cytokine detection reagents, and can solve the problems of difficult combination of cytokines, difficult to achieve auxiliary diagnosis and typing of ophthalmic diseases, etc.

Pending Publication Date: 2021-05-28
博真医学检验实验室(浙江)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For the detection of cytokines in intraocular fluid, there is currently a problem that the combination of cytokines is difficult to match, and it is difficult to achieve auxiliary diagnosis and typing of ophthalmic diseases

Method used

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  • Intraocular fluid cytokine detection reagent, detection method and application
  • Intraocular fluid cytokine detection reagent, detection method and application
  • Intraocular fluid cytokine detection reagent, detection method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] A detection reagent for intraocular fluid cytokines used for auxiliary diagnosis of inflammatory eye diseases, using HumanEotaxin Flex Set, Human IL-10 Flex Set, Human IL-12p70 Flex Set, Human IL-1βFlex Set, Human IL-6 Flex Set , Human IL-8 Flex Set, Human MCP-1 Flex Set, HumanTNF Flex Set, Human IFN-γ Flex Set and Human VEGF Flex Set detection reagents.

[0066] The detection method comprises the following steps:

[0067] Intraocular fluid sample preparation: take the vitreous humor and centrifuge it at 10000rpm for 10min to obtain the intraocular fluid sample;

[0068] Preparation of microsphere solution: Take 0.25 μl of each microsphere reagent and mix, add 10 μl of microsphere diluent, and mix well to obtain a microsphere solution;

[0069] Preparation of antibody solution: Take 0.25 μl of each antibody reagent and mix, add 10 μl of antibody diluent, mix well to obtain antibody solution;

[0070] Sample incubation: put the microsphere solution into a blank flow tu...

Embodiment 2

[0075] A method for detecting intraocular fluid cytokines for auxiliary diagnosis of immune eye diseases, using Human IL-1β Flex Set, Human IL-2 Flex Set, Human IL-5 Flex Set, Human IL-6 Flex Set, HumanTNF Flex Set , Human IFN-γ Flex Set, Human VEGF Flex Set, Human IL-17a Flex Set and Human TGF-β1 Single Plex Flex Set detection reagents.

[0076] The method for detecting cytokines other than TGF-β1 includes the following steps:

[0077] Intraocular fluid sample preparation: take aqueous humor and centrifuge at 10000rpm for 10min to obtain intraocular fluid sample;

[0078] Preparation of microsphere solution: Take 0.25 μl of each microsphere reagent except TGF-β1 and mix them, add 10.5 μl of microsphere diluent, and mix well to obtain a microsphere solution;

[0079] Antibody solution preparation: Take 0.25 μl of each antibody reagent except TGF-β1 and mix, add 10.5 μl of antibody diluent, mix well to obtain antibody solution;

[0080] Sample incubation: put the microsphere ...

Embodiment 3

[0094] A method for detecting intraocular fluid cytokines for auxiliary diagnosis of vascular eye disease, using Human IL-10 Flex Set, Human IL-6 Flex Set, Human IL-8 Flex Set, Human TNF Flex Set, HumanVEGF Flex Set, Human Soluble CD54 (ICAM-1) Flex Set, Human Soluble CD106 (VCAM-1) Flex Set, Human TGF-β1 Single Plex Flex Set detection reagents.

[0095] The method for detecting cytokines other than TGF-β1 includes the following steps:

[0096] Intraocular fluid sample preparation: take aqueous humor and centrifuge at 10000rpm for 10min to obtain intraocular fluid sample;

[0097] Preparation of microsphere solution: Take 0.25 μl of each microsphere reagent except TGF-β1 and mix them, add 10.75 μl of microsphere diluent, and mix well to obtain a microsphere solution;

[0098] Preparation of antibody solution: Take 0.25 μl of each antibody reagent except TGF-β1 and mix them, add 10.75 μl of antibody diluent, mix well to obtain antibody solution;

[0099] Sample incubation: pu...

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Abstract

The invention relates to the technical field of auxiliary diagnosis of diseases, and particularly discloses an intraocular fluid cytokine detection reagent, a detection method and application. The detection reagent comprises at least two of the reagents for detecting Eotaxin, IL-10, IL-12p70, IL-1[beta], IL-2, IL-5, IL-6, IL-8, MCP-1, TNF, IFN-gamma, VEGF, IL-17a, ICAM-1, VCAM-1, TGF-[beta]1 and bFGF in the intraocular fluid. The detection method comprises the following steps: 1, uniformly mixing an intraocular fluid sample and a microsphere solution; 2, uniformly mixing the premixed sample and the antibody solution; 3, carrying out fluorescence detection on the to-be-detected sample through a flow cytometer. According to the application, the seventeen cell factors are screened and combined, so that the requirements of auxiliary diagnosis and typing of ophthalmic diseases can be met.

Description

technical field [0001] The present application relates to the field of aided diagnosis technology for diseases, more specifically, it relates to a detection reagent, detection method and application of intraocular fluid cytokines. Background technique [0002] Cytokines are a class of small molecular proteins with a wide range of biological activities synthesized and secreted by immune cells and certain non-immune cells upon stimulation, and are important mediators of interactions between cells. The interaction of many cytokines constitutes a cytokine network characterized by pleiotropy and overlap. Pleiotropy means that one cytokine may have many different functions, while overlap means that certain cytokines have common activities. [0003] Changes in cytokine content can assist in the diagnosis and typing of diseases, so cytokine detection is one of the commonly used detection methods. For example: intraocular fluid detection is a means of ophthalmic disease diagnosis. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/543
CPCG01N33/6866G01N33/6869G01N33/6863G01N33/6872G01N33/6893G01N33/582G01N33/54313G01N2333/52G01N2333/5428G01N2333/5434G01N2333/545G01N2333/5409G01N2333/55G01N2333/5412G01N2333/5421G01N2333/57G01N2333/525G01N2333/523G01N2333/495G01N2333/503G01N2333/70525G01N2800/16G01N2800/164
Inventor 倪万茂迟妍妍陈慧陈乐芝陈鹏贵林鹏程林天鹏朱阳承
Owner 博真医学检验实验室(浙江)有限公司
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