A high-efficiency actinomycete separation culture device

Abstract

A high-efficiency actinomycete separation culture device comprises a support, a plurality of vertical rails on the right side of the support, sliders on the vertical rails, a circular ring at the upper end of the sliders, a dilution tube on the inner ring of the circular ring, a dropper at the bottom of the dilution tube, a first one-way pressure valve in the dropper, a first piston on the dropper, a second through hole on the first piston, and a first electromagnetic valve connected with the second through hole. The first electromagnetic valve is connected with a sterile water supply pipe, the slider is connected with a driving device, and an electric push rod is arranged at the upper end of the support. A controller is also included. In use, the invention is controlled by the controller, sterile water in all the dilution pipes can be automatically supplemented, the dilution ratio of actinomycetes in all the dilution pipes can be ensured to be constant, and the problem of overlarge error caused by single dilution is avoided through a sequential dilution method, so that the device is applicable to supply of a plurality of culture medium plates; especially, the assembly line of a production workshop is adapted, and supplement of solutions of the dilution tubes on different layers and liquid outlet of the dropper on the lowermost side do not conflict with each other.

Description

technical field [0001] The invention belongs to the field of culture devices, in particular to a high-efficiency separation and culture device for actinomycetes. Background technique [0002] Actinomycetes is a special group of prokaryotes that can form branched hyphae and conidia. It grows in the form of mycelium and reproduces mainly by spores. It is named because the colony is radial. Most have well-developed branched hyphae. The hyphae are slender, and the width is close to that of rod-shaped bacteria, about 0.2-1.2 microns. Can be divided into: vegetative mycelium, also known as basal mycelium or primary mycelium, the main function is to absorb nutrients, some can produce different pigments, which is an important basis for bacterial species identification; aerial mycelium, superimposed On the vegetative mycelia, it is also called secondary mycelium; spores, aerial mycelia develop to a certain stage, and mycelia that form spores can be differentiated on it. The cultiv...

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Problems solved by technology

The cultivation process of actinomycetes needs to select soil samples to make soil suspension, then dilute the soil suspension by a certain number of times, and finally drop the diluted actinomycetes solution on the medium plate, and finally put it on the petri dish, place After 3-4 days in an incubator at 28°C, the tube can be transferred. However, the traditional operation of the whole process is quite complicated, and it is necessary to use a dropper to absorb the solution for transfer. Not only is the precision not enough, but it is also easy to contaminate other bacteria and cannot Realize streamlined production

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