Gamma-aminobutyric acid lactobacillus plantarum leavening agent and application thereof in fermentation of pickled vegetables

A technology of Lactobacillus plantarum and aminobutyric acid, applied in the field of starter, can solve the problems of single variety of kimchi, no health care effect, low nutritional content, etc., achieve food safety, promote liver function and kidney function, and improve mental depression. Effect

Pending Publication Date: 2021-06-01
漯河微康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in one aspect of the present invention is to provide a γ-aminobutyric acid lactobacillus plantarum starter, which solves the technical problems in the prior art that the variety of kimchi is single, the nutritional content is not high, and it does not have a health care effect, and achieves The technical effects of cost saving, simple process operation, safe food and functional health care

Method used

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  • Gamma-aminobutyric acid lactobacillus plantarum leavening agent and application thereof in fermentation of pickled vegetables
  • Gamma-aminobutyric acid lactobacillus plantarum leavening agent and application thereof in fermentation of pickled vegetables

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Effect test

Embodiment 1

[0034] Preparation of Lactobacillus plantarum 29MJ-2 direct-throwing starter:

[0035] A. Lactobacillus plantarum 29MJ-2 preserved in glycerol tubes was inoculated into MRS liquid medium at a ratio of 2%, cultured statically at 37° C. for 16 hours, and activated for 3 generations.

[0036] B. Inoculate the activated seeds into the fermenter at 2%, culture at 37°C for 12 hours, control the pH to 5.8, centrifuge the bacterial solution at 10,000r / min for 15 minutes, discard the supernatant, collect the bacterial sludge, add trehalose and malt paste Essence, sucrose, mannitol, and Tween-80 are used as a mixed protective agent, and then vacuum freeze-dried to prepare a direct-throwing starter.

[0037] Among them, the components in the Lactobacillus plantarum 29MJ-2 direct-injection starter are, by mass percentage: 65% of Lactobacillus plantarum, 20% of trehalose, 10% of maltodextrin, 3% of sucrose, 0.2% of mannitol, and Temperature-80 0.2%, the number of viable Lactobacillus plan...

Embodiment 2

[0045] Preparation of Lactobacillus plantarum 29MJ-2 direct-throwing starter:

[0046]A. Lactobacillus plantarum 29MJ-2 preserved in glycerol tubes was inoculated into MRS liquid medium at a ratio of 2%, cultured statically at 37° C. for 16 hours, and activated for 3 generations.

[0047] B. Inoculate the activated seeds into the fermenter at 2%, culture at 37°C for 12 hours, control the pH to 5.8, centrifuge the bacterial solution at 6000r / min for 15 minutes, discard the supernatant, collect the bacterial sludge, add trehalose and malt paste Essence, sucrose, mannitol, and Tween-80 are used as a mixed protective agent, and then vacuum freeze-dried to prepare a direct-throwing starter.

[0048] Among them, the components in the Lactobacillus plantarum 29MJ-2 direct-injection starter are, by mass percentage: 65% of Lactobacillus plantarum, 20% of trehalose, 10% of maltodextrin, 3% of sucrose, 0.2% of mannitol, and Temperature-80 0.2%, the number of viable Lactobacillus plantar...

Embodiment 3

[0056] Preparation of Lactobacillus plantarum 29MJ-2 direct-throwing starter:

[0057] A. Lactobacillus plantarum 29MJ-2 preserved in glycerol tubes was inoculated into MRS liquid medium at a ratio of 2%, cultured statically at 37° C. for 16 hours, and activated for 3 generations.

[0058] B. Inoculate the activated seeds into the fermenter at 2%, culture at 37°C for 12 hours, control the pH to 5.8, centrifuge the bacterial solution at 10,000r / min for 15 minutes, discard the supernatant, collect the bacterial sludge, add trehalose and malt paste Essence, sucrose, mannitol, and Tween-80 are used as a mixed protective agent, and then vacuum freeze-dried to prepare a direct-throwing starter.

[0059] Among them, the components in the Lactobacillus plantarum 29MJ-2 direct-injection starter are, by mass percentage: 65% of Lactobacillus plantarum, 20% of trehalose, 10% of maltodextrin, 3% of sucrose, 0.2% of mannitol, and Temperature-80 0.2%, the number of viable Lactobacillus plan...

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Abstract

The invention discloses a gamma-aminobutyric acid lactobacillus plantarum leavening agent. The lactobacillus plantarum leavening agent is prepared from lactobacillus plantarum, trehalose, maltodextrin, cane sugar, mannitol and tween-80. The pickled vegetables are prepared by fermenting the gamma-aminobutyric acid lactobacillus plantarum leavening agent prepared by adopting the preparation method of the gamma-aminobutyric acid lactobacillus plantarum leavening agent. Gamma-aminobutyric acid is prepared through a high-yield strain fermentation method and applied to food, the use is safe, the cost is low, pollution is small, the yield is high, the content of gamma-aminobutyric acid in the prepared pickled vegetables is high. The prepared pickled vegetables are rich in gamma-aminobutyric acid, the effects of reducing blood pressure, reducing cholesterol absorption, regulating lipid metabolism, promoting ethanol metabolism and regulating nerves can be achieved; insomnia and deprementia can be improved, liver and kidney functions can be effectively promoted, and senescence is delayed.

Description

technical field [0001] The invention relates to the technical field of starters, in particular to a plant lactobacillus starter, especially a gamma-aminobutyric acid plant lactobacillus starter and the application of the fermented kimchi. Background technique [0002] γ-aminobutyric acid (γ-aminobutyric acid, referred to as GABA) is converted from glutamic acid by glutamic acid decarboxylase, and is an inhibitory neurotransmitter substance present in the brain and spinal cord of mammals. It has the effects of sedation, hypnosis, anticonvulsion, lowering blood pressure, improving brain function, promoting long-term memory, as well as promoting the secretion of growth hormone, activating kidney function, and liver function. On September 27, 2009, the Ministry of Health approved γ-aminobutyric acid as a new resource food. [0003] At present, the methods for obtaining GABA mainly include chemical synthesis, plant enrichment, and microbial fermentation. Among them, the chemica...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04A23L19/20A23L29/00A23L33/00C12R1/25
CPCC12N1/20C12N1/04A23L19/20A23L29/065A23L33/00A23V2002/00A23V2400/169A23V2200/326A23V2200/3262A23V2200/30A23V2200/322A23V2200/302A23V2250/636A23V2250/628A23V2250/6418A23V2250/5114
Inventor 方曙光武岩峰汪欣朱建国
Owner 漯河微康生物科技有限公司
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