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Large-week-age female mouse egg cell in-vitro fertilization nutrient solution and application thereof

An in vitro fertilization, egg cell technology, applied in the direction of cell culture active agents, animal cells, germ cells, etc., to prolong the time of maintaining fertilization ability, delay the aging process, and improve fertilization ability.

Active Publication Date: 2021-06-01
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of scientific research, it is impossible to provide uniform 3-4 week-old female donor eggs for microbiological purification and sperm recovery operations. How to improve the fertilization and development ability of egg cells provided by older female mice under in vitro conditions has become a challenge Urgent problems to be solved in mouse assisted reproductive technology

Method used

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  • Large-week-age female mouse egg cell in-vitro fertilization nutrient solution and application thereof
  • Large-week-age female mouse egg cell in-vitro fertilization nutrient solution and application thereof
  • Large-week-age female mouse egg cell in-vitro fertilization nutrient solution and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A nutrient solution for in vitro fertilization of egg cells of older female mice, consisting of the following components: NaCl 5.5g, KCl 0.3g, KH 2 PO 4 0.045g, MgSO 4 ·7H 2 O 0.045g, sodium lactate 2.35g, sodium pyruvate 0.03g, glucose 0.4g, NaHCO 3 2.0g, CaCl 2 2H 2 O 0.25g, BSA3.0g, melatonin 1×10 -10 mol, made up to 1L with ultrapure water.

Embodiment 2

[0030] A nutrient solution for in vitro fertilization of egg cells of older female mice, consisting of the following components: NaCl 6.2g, KCl 0.4g, KH 2 PO 4 0.05g, MgSO 4 ·7H 2 O 0.05g, sodium lactate 2.43g, sodium pyruvate 0.04g, glucose 0.6g, NaHCO 3 2.2g, CaCl 2 2H 2 O 0.35g, BSA 5.0g, melatonin 1×10 -8 mol, made up to 1L with ultrapure water.

Embodiment 3

[0032] A nutrient solution for in vitro fertilization of egg cells of older female mice, consisting of the following components: NaCl 5.7g, KCl 0.32g, KH 2 PO 4 0.047g, MgSO 4 ·7H 2 O 0.047g, sodium lactate 2.38g, sodium pyruvate 0.034g, glucose 0.45g, NaHCO 3 2.1g, CaCl 2 2H 2 O 0.28g, BSA 3.5g, melatonin 6×10 -9 mol, made up to 1L with ultrapure water.

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Abstract

The invention provides a large-week-age female mouse egg cell in-vitro fertilization nutrient solution and application thereof, and belongs to the technical field of in-vitro fertilization of rats. The large-week-age female mouse egg cell in-vitro fertilization nutrient solution is prepared from small-week-age female mouse egg cell in-vitro fertilization liquid and melatonin. According to the invention, a certain dose of melatonin is added into the in-vitro insemination liquid for the egg cells of the small-week-age female rats, so that the in-vitro aging process of the eggs of the large-week-age female rats can be delayed, and the time for maintaining the fertilization ability is prolonged, thereby improving the fertilization ability and improving the success rate of microbiological purification and strain reconstruction.

Description

technical field [0001] The invention belongs to the technical field of in vitro fertilization of mice, and in particular relates to a nutrient solution for in vitro fertilization of egg cells of older female mice and an application thereof. Background technique [0002] With the application of genetic modification technology, tens of thousands of genetically modified mouse models have been produced in laboratories around the world, and the use of genetically modified mouse models to communicate in laboratories around the world has become a norm, so genetically modified mouse models Microbiological purification, recovery of sperm cryopreservation, and off-site reconstruction of strains have also become the norm. In vitro fertilization has become an important technology to support the purification and strain reconstruction of these normalized mouse models. [0003] In vitro fertilization is to simulate the environment in the body, in which sperm and eggs are fertilized and de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/075
CPCC12N5/0609C12N2501/30
Inventor 马雪云刘腾飞章延嘉
Owner EAST CHINA NORMAL UNIV