Chitosan-sodium alginate composite scaffold loaded with egcg nanoparticles and preparation method thereof
A technology of sodium alginate and nanoparticles, which can be used in pharmaceutical formulations, prostheses, capsule delivery, etc. It can solve the problems of inability to achieve long-term functionality, unfavorable transport of nutrients and gases, and reduce the porosity of scaffold materials, so as to promote Proliferation of mesenchymal stem cells, simple and convenient preparation method, and reduced preparation cost
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[0028] In addition, the present invention also provides a preparation method of a chitosan-sodium alginate composite scaffold loaded with EGCG nanoparticles, comprising the following steps: S1) preparing chitosan loaded with EGCG with a mass concentration of 2 mg / ml-8 mg / ml Suspension of sugar nanoparticles; in the step S1), including S11) EGCG is added to the chitosan solution with a mass concentration of 1 mg / ml to 10 mg / ml and fully mixed, so that the mass ratio of EGCG to chitosan is 1 :25~1:100, obtain chitosan-EGCG mixed solution, and adjust the pH value of described chitosan-EGCG mixed solution to 4~5.5; S12) sodium triphosphate solution is added dropwise to described chitosan - In the EGCG mixed solution, the volume ratio of the chitosan-EGCG mixed solution to the sodium triphosphate solution is 7:3 to 8:2, and the mass concentration of the sodium triphosphate solution is 1 mg / ml to 4 mg / ml; Stir continuously for 1 hour, and the formed nano-suspension is centrifuged at...
Embodiment 1
[0032] see figure 1 , this embodiment 1 provides a preparation method of chitosan-sodium alginate composite scaffold loaded with nanoparticles, which is used for the proliferation of mesenchymal stem cells, including step S1)'-step S3)'.
[0033] specific
[0034] S1)'preparing a chitosan solution with a mass concentration of 5mg / ml, adjusting the pH of the chitosan solution to 4.8 with a concentration of 1N NaOH to obtain a chitosan mixture. A sodium triphosphate solution with a mass concentration of 2 mg / ml was added dropwise into the chitosan mixture, wherein the volume ratio of the sodium triphosphate solution to the chitosan mixture was 1:4. After continuous stirring for 1 hour, place it in a centrifuge and centrifuge at a speed of 15,000 rpm for 30 minutes, wash twice with distilled water, put it into -80°C for quick freezing, and then freeze-dry it at -20°C for 24 hours to prepare chitosan nanoparticles. Chitosan nanoparticles were added into purified water to prepar...
Embodiment 2
[0042] see figure 2 , a method for preparing a chitosan-sodium alginate composite scaffold loaded with EGCG nanoparticles in this embodiment, comprising step S1)-step S3).
[0043] specific
[0044] S1) Prepare a suspension of chitosan nanoparticles loaded with EGCG at a mass concentration of 4 mg / ml; this step includes step S11) to step S13). see image 3 , S11) prepare chitosan-EGCG mixed solution, be about to add EGCG mass concentration and be in the chitosan solution of 5mg / ml, until the concentration of EGCG reaches 25 μ M, and fully mix, then regulate chitosan with the NaOH that concentration is 1N The pH of the solution reaches 4.8 to obtain the chitosan-EGCG mixed solution. S12) The sodium triphosphate solution was added dropwise to the chitosan-EGCG mixed solution, and after continuous stirring for 1 h, the nanoparticle suspension formed was centrifuged at 15,000 rpm for 30 minutes with a high-speed centrifuge, and then washed twice with distilled water , placed ...
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Abstract
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