An Antimony Oxidizing Bacteria with High Resistance and High Oxidation Ability and Its Application

A technology for oxidizing bacteria and trivalent antimony, which is applied in the direction of bacteria, chemical instruments and methods, and the restoration of contaminated soil, can solve the problems of lack of Sb oxidation function microorganisms and low oxidation efficiency, so as to reduce the risk of ecological environment, Good application prospect, low cost effect

Active Publication Date: 2022-04-05
GUANGXI UNIV
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  • Abstract
  • Description
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Problems solved by technology

However, according to the current research and published related patents, the discovery of Sb oxidation functional microorganisms is still very lacking, and the oxidation efficiency is low.

Method used

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  • An Antimony Oxidizing Bacteria with High Resistance and High Oxidation Ability and Its Application
  • An Antimony Oxidizing Bacteria with High Resistance and High Oxidation Ability and Its Application
  • An Antimony Oxidizing Bacteria with High Resistance and High Oxidation Ability and Its Application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Isolation and purification of Sb(III) oxidizing bacteria

[0029] (1) Enrichment: In order to obtain strains with Sb tolerance and oxidation function from the environment, farmland soil that had been heavily polluted by Sb for a long time was collected. Prepare 100ml of LB liquid medium (10g / L peptone, 10g / L NaCl, 5g / L yeast) in a 250ml Erlenmeyer flask, after the medium is sterilized, cool and add sterile Sb(III) solution to make the medium The concentration of Sb(III) in the medium was kept at 5mM (ie 0.6g / L). Take 1 g of fresh soil sample and add it to the above-mentioned medium, and cultivate it on a constant temperature air bath shaker at 30° C. with a rotation speed of 150 rpm for 48 hours. Every 24 hours, 5 mL of the aforementioned enriched culture solution was transferred into sterilized liquid LB medium with the same antimony concentration, and repeated three times.

[0030] (2) Plate separation: Take 1 mL of the third enrichment culture solutio...

Embodiment 2

[0037] Example 2: Growth characteristics of Klebsiella aerogenes X

[0038] Preparation of strain seed liquid: Inoculate the strain in LB liquid medium and cultivate for 12 hours, centrifuge at 8000r / min for 10 minutes, collect the bacteria, wash twice with 0.8% NaCl solution and resuspend, resuspend and adjust OD=1.0, Conduct the following research.

[0039] (1) The influence of temperature on bacterial strain growth: by the inoculum of 1% (v / v), inoculate seed solution in the Erlenmeyer flask that 100ml LB culture medium (regulating pH 7.2) is housed, under different temperature (25 ℃, 30°C, 35°C), cultured on a shaker at 150r / min, samples were taken at different time points to measure the Optical Density 600 (OD600) of the solution, and each treatment was repeated 3 times. The growth curves of Klebsiella aerogenes X at different temperatures are shown in Figure 4 . It can be seen from the figure that the growth rate of the strain in the logarithmic phase increases with ...

Embodiment 3

[0044] Example 3: Properties of Klebsiella aerogenes X oxidation of Sb(III)

[0045] In order to understand the oxidative characteristics of the strains under the stress of different Sb(III) concentrations, the seed solution (1%) was inoculated at 1.21mg / L (0.01mM), 6.09mg / L (0.05mM) and 12.18mg / L (0.1mM) In the LB liquid medium with Sb(III) concentration, samples were taken at different time points, the concentration of Sb(III) in the solution and the change of cell concentration (OD600) were measured, and the oxidation rate was calculated. The calculating formula of oxidation rate is the same as embodiment 1 (3).

[0046] The oxidation of Sb(III) in aqueous solution and the change of cell concentration by Klebsiella aerogenes X at different culture time are shown in Figure 8 . During the sampling time, with the prolongation of time, the oxidation rate gradually increased, of which 6.09mg / L+Klebsiella aerogenes X( Figure 8 B) The oxidation rate of Sb(III) can reach more ...

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Abstract

The invention discloses an antimony-oxidizing bacterium with high tolerance and strong oxidation ability, which is Klebsiella aerogenes, the preservation number is CGMCC NO: 20508, and the classification name is Klebsiella aerogenes X. Studies have shown that the strain's tolerance to Sb(III) in solution is as high as 40mM (4.8g / L), and can significantly reduce the content of Sb(III) in aqueous solution. It can be used to treat high-concentration Sb(III) wastewater. The Sb(III) in it is oxidized to Sb(V), and the oxidation rate can reach 98.5%, which significantly reduces the toxicity of Sb in the environment. Using the antimony oxidizing bacteria with high tolerance and strong oxidation ability of the present invention to carry out biological oxidation of wastewater containing Sb(III), compared with traditional chemical oxidation, has many characteristics such as low cost, high efficiency, and no secondary pollution, and can greatly reduce the production of antimony. Therefore, it has a good application prospect in bioremediation of Sb(III)-contaminated wastewater.

Description

technical field [0001] The invention belongs to the technical field of microbial remediation of environmental heavy metal pollution, in particular to an antimony oxidizing bacterium with high tolerance and strong oxidation ability and application thereof. Background technique [0002] Antimony (Sb) is a metalloid with high toxicity and carcinogenicity. It is a congener element with arsenic, and its chemical properties are similar to arsenic. my country is the country with the largest output of antimony in the world, accounting for 65% of the world's total. The mining of antimony ore has caused serious environmental pollution, and production activities such as mining, dressing and smelting have resulted in a large amount of Sb in the water body. Soil pollution caused by pollution is also becoming more and more serious. The toxicity of antimony and its compounds has aroused extensive research and discussion around the world. Antimony mainly exists in the form of inorganic com...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/22C02F101/20
CPCC02F3/34B09C1/10C02F2101/20
Inventor 容群张超兰赵贺诚秦兴姿钟凯农欣瑜
Owner GUANGXI UNIV
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