Vector and preparation for knocking down human CBS gene as well as preparation method and application thereof

A carrier and gene technology used in the field of biopharmaceuticals to inhibit cell growth and reduce the migration rate of cancer cells
CN112980845APending Publication Date: 2021-06-18HENAN UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HENAN UNIVERSITY
Publication Date
2021-06-18

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Abstract

The invention discloses a vector and a preparation for knocking down a human CBS gene as well as a preparation method and application thereof, and relates to the technical field of biological pharmacy. The preparation method of the vector comprises the following steps: designing a primer for PCR amplification of a target gene according to a human CBS gene sequence, and the specific sequence is as shown in SEQ ID NO.1; restriction sites BamH I and Hind III in the GV102 vector are digested to form a linearized GV102 vector, and the sequence of the GV102 vector is hU6-MCS-CMV-GFP-SV40-Neomycin; and connecting the products obtained in the previous two steps to construct a GV102-CBS vector. The vector prepared by the invention can obviously inhibit the expression of human CBS genes, achieve the purposes of inhibiting the growth of human thyroid cancer cells and reducing the migration rate of the cancer cells, and can be used for preparing a preparation for treating the human thyroid cancer.
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Description

technical field

[0001] The invention relates to the technical field of biopharmaceuticals, in particular to a carrier for knocking down human CBS gene, a preparation, a preparation method and application thereof. Background technique

[0002] Thyroid carcinoma (thyroid carcinoma) is the most common malignant tumor of the endocrine system and belongs to the tumor of the thyroid epithelium. It includes four pathological types, namely papillary carcinoma, follicular carcinoma, undifferentiated carcinoma and medullary carcinoma. With the exception of medullary carcinoma, approximately 90% of thyroid cancers arise from follicular epithelial cells. Papillary carcinoma is the most common type of thyroid cancer with low malignancy and good prognosis. At present, the treatment methods for thyroid cancer at home and abroad mainly include surgery, thyrotropin suppression therapy, biological therapy and traditional Chinese medicine therapy. However, there is no report on the treatment...

Claims

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