Method for evaluating virus inactivation efficiency of virus inactivation mask
A virus inactivation and face mask technology, which is applied in biochemical equipment and methods, and microbial measurement/inspection, can solve the problems that the inactivation effect of virus inactivation masks cannot be evaluated, so as to promote development and innovation, reduce the risk of infection, and ensure authenticity effect
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[0055] Example 1
[0056] Taking the virus as a Sindbis Virus as an example, a method for evaluating the inactivation efficiency of viral inactivation masks to virus (Sindz virus), corresponding host cells are Vero cells, including the following steps:
[0057] S1. After the long-term Vero cells are digested and counted, the MEM medium containing 10% fetal bovine serum is added, and the cell density is adjusted to 5 × 10. 5 A / ml, obtained cell suspension, taking 15 ml of cell suspension to T75 cell culture flask after 37 ° C, 5% CO 2 The culture tank culture, when the cells reached a 90% conversion state, the medium in the T75 cell culture flask was abandoned, and 13 ml of MEM medium containing 10% fetal bovine serum was added, and the cells were continued to the superhui state;
[0058] Abandoning the medium in the T75 cell culture bottle, first add 1 ml of Sindz virus liquid to T75 cell culture flask, and then add 3ml of MEM medium containing 10% fetal bovine serum, and shake ...
Example Embodiment
[0076] Example 2
[0077] A method of evaluating viral inactivation mask to virus (Sinkbs virus) inactivation efficiency, corresponding host cells are Vero cells, including the following steps:
[0078] S1. After the long-term Vero cells are digested and counted, the MEM medium containing 10% fetal bovine serum is added, and the cell density is adjusted to 5 × 10. 5 A / ml, obtained cell suspension, taking 15 ml of cell suspension to T75 cell culture flask after 37 ° C, 5% CO 2 The culture tank culture, when the cells reached a 90% conversion state, the medium in the T75 cell culture flask was abandoned, and 14 ml of MEM medium containing 10% fetal bovine serum was added, and the cells were continued to the superhui state;
[0079] Abandoning the medium in the T75 cell culture flask, 1 ml of Sindz viral solution in T75 cell culture flask, then add 3.5ml of MEM medium containing 10% fetal bovine serum, and shake T75 cell culture bottles to make Sindz virus After sufficient contact ...
Example Embodiment
[0097] Example 3
[0098] A method of evaluating viral inactivation mask to virus (Sinkbs virus) inactivation efficiency, corresponding host cells are Vero cells, including the following steps:
[0099] S1. After the long-term Vero cells are digested and counted, the MEM medium containing 10% fetal bovine serum is added, and the cell density is adjusted to 5 × 10. 5 A / ml, obtained cell suspension, taking 15 ml of cell suspension to T75 cell culture flask after 37 ° C, 5% CO 2 The culture tank culture, when the cells reached a 90% conversion state, the medium in the T75 cell culture flask was discarded, and 15 ml of MEM medium containing 10% fetal bovine serum was added, and the cells were continued to the superhui state;
[0100] Abandoning the medium in the T75 cell culture flask, 1 ml of the Sindz virus fluid in T75 cell culture flask, then add 4ml of MEM medium containing 10% fetal bovine serum, and shake T75 cell culture bottle to make Sindbis virus fluid After sufficient co...
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