Lactobacillus reuteri and its use, composition, medicine and food
A technology of Lactobacillus reuteri and a composition, applied in the field of microorganisms, can solve problems such as protein exudation, allergic reaction and the like
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[0040] The above-mentioned Lactobacillus reuteri preparation method adopts high-density fermentation to prepare the above-mentioned Lactobacillus reuteri, and the yield is high.
[0041] In addition, based on the above-mentioned functions of the above-mentioned Lactobacillus reuteri, an embodiment of the present invention also provides a composition comprising the above-mentioned Lactobacillus reuteri and / or the fermentation product of the above-mentioned Lactobacillus reuteri.
[0042] Specifically, the fermentation product of the above-mentioned Lactobacillus reuteri refers to the culture product of the above-mentioned Lactobacillus reuteri, including at least one of metabolites located inside the bacteria and metabolites secreted outside the bacteria. When in use, take the culture fluid after the cultivation of the Lactobacillus reuteri or the freeze-dried powder obtained by freeze-drying the culture fluid. Alternatively, take the lysate lysed after the culture of Lactobaci...
Embodiment 1
[0064] A strain was isolated from the milk of healthy women by anaerobic culture. After 16S rRNA full-length sequencing and mass spectrometry identification, the strain was a new strain belonging to Lactobacillus reuteri and named Lactobacillus reuteri LR99 (referred to as "LR99" or "LR-99"). The strain has been deposited in the General Microbiology Center of China Committee for Culture Collection of Microorganisms (CGMCC for short, Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Zip code 100101) on December 31, 2020. , the classification name is Lactobacillus reuteri (Lactobacillus reuteri), and the preservation number is CGMCC No.21577.
[0065] 1. Taxonomic characteristics of Lactobacillus reuteri LR99:
[0066] (1) Observing Lactobacillus reuteri LR99 under a microscope, the results are as follows figure 1 shown.
[0067] (2) The physical and chemical test results are shown in Tables 1 and 2....
Embodiment 2
[0090] This example is used to illustrate the high-density fermentation of Lactobacillus reuteri LR99 and the preparation process of freeze-dried bacterial powder.
[0091] (1) Lactobacillus reuteri LR99 is anaerobically cultured in a modified MRS medium to obtain fermentation strains.
[0092] (2) Carry out three activation cultures to the Lactobacillus reuteri LR99 strain to improve the viability of the strain. The temperature of the three activation cultures is 37 ° C, and the time is between 16h and 18h. After the activation of the strain is completed, the Lactobacillus reuteri LR99 The strains were tested for growth curve, and the results are shown in Table 4.
[0093] Table 4
[0094] time pH OD 5h 4.48 4.5 6h 4.31 4.71 7h 4.17 6.05 8h 4.06 6.97 9h 3.73 7.26 10h 3.67 7.78
[0095] From the detection results of the growth curve of Lactobacillus reuteri LR99, it can be seen that the time point when Lactobacillus reute...
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