Method for quickly determining nature of manuka honey

A manuka honey, rapid technology, applied in the detection of manuka honey, the detection field, can solve the problems of dependence, inability to realize on-site, rapid detection, different levels, etc.

Pending Publication Date: 2021-07-06
南京海关动植物与食品检测中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the widely used detection technology is to detect specific characteristic markers, but this method has its disadvantages
First, this method relies on the precise separation of samples, so it usually needs to be equipped with high-performance liquid chromatography and other equipment in the detection, which is not only expensive but also must be sent to a specific laboratory for inspection, which cannot achieve on-site and rapid detection; Finally, the purest Manuka honey comes from the single-flower

Method used

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  • Method for quickly determining nature of manuka honey
  • Method for quickly determining nature of manuka honey
  • Method for quickly determining nature of manuka honey

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Sample pretreatment:

[0024] Accurately weigh 1.000g of the sample, place it in a 50mL polytetrafluoroethylene centrifuge tube with a cover, dilute with high-purity water to 50mL, vortex and oscillate to mix, take about 5mL of the supernatant, and pass through a 0.45μm nylon microporous filter membrane. Waiting for instrument detection.

[0025] Instrument measurement conditions:

[0026] Excitation wavelength: 270nm; Excitation slit: 10nm; Emission slit: 10nm; Emission spectrum scanning range: 280nm~550nm; Scanning speed: 1200nm / min.

[0027] Such as figure 1 As shown in , Manuka monofloral honey has a fluorescence peak at 305nm and 360nm respectively, but the intensity of the characteristic fluorescence peak at 360nm is more than twice the intensity of the fluorescence peak at 305nm; as figure 2 The Manuka multifloral honey shown in has an obvious fluorescence peak at 305nm and 360nm respectively, and the difference in fluorescence intensity between the two is no...

Embodiment 2

[0029] According to the method in Example 1, a total of 14 manuka, kanuka, clover, Christmas flower, rivarewa, manna, etc. from different origins in New Zealand were collected from the hive by using the molecular fluorescence spectroscopic analysis method. The mature raw honey samples were tested.

[0030] A total of 13 samples of 6 different varieties, including manuka, kanuka, clover, Christmas flower, rivarewa, and honeydew honey, were numbered and the corresponding honey types were recorded.

[0031] Then according to the method in embodiment 1, use molecular fluorescence spectrum analysis method to detect above-mentioned 13 samples, and test result is compared with known and recorded honey kind, detection and comparison result are as shown in table 1,

[0032] Table 1:

[0033]

[0034]

[0035] The results in Table 1 show that the results of the detection method disclosed by us are consistent with the actual samples, indicating that the detection method disclosed ...

Embodiment 3

[0037] 42 commercial honey samples from New Zealand and Australia were detected according to the molecular fluorescence spectrum analysis method disclosed in the present invention (Example 1) and the pollen DNA detection method officially released by the New Zealand Ministry of Primary Industries, and the detection results are shown in Table 2 ;

[0038] Table 2:

[0039]

[0040]

[0041] According to Table 2, it can be seen that among the 20 commercial honeys marked as Manuka from New Zealand, 18 samples are Manuka honey and monofloral Manuka honey, and 2 samples are non-Manuka honey; Of the 2 commercial honeys from New Zealand marked as Manuka blend honey, 1 sample was multiflora manuka honey and the other was non-manuka honey; 2 commercial honeys from Australia marked as Manuka Among the commercialized honeys, one sample is Manuka honey, and it is monofloral Manuka honey, and the other is non-Manuka honey; a total of 18 commercial honeys from New Zealand and Austral...

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Abstract

The invention relates to the field of detection, in particular to the field of detection of manuka honey, and more particularly relates to a method for quickly determining the nature of manuka honey, which is a molecular fluorescence method and comprises the following steps of: irradiating a sample to be detected by using 270nm as an excitation wavelength, scanning an emission spectrum in a range of 280-550nm, and judging by using fluorescence peaks at 305nm and 360nm and the intensity of the fluorescence peaks. The qualitative detection method disclosed by the invention can be used for rapidly, accurately and qualitatively detecting single-flower manuka honey, multi-flower manuka honey and other adulterated honey. According to the method, expensive instruments such as a high performance liquid chromatograph and the like and complicated separation treatment are not needed, so that the manual honey does not need to be sent to a specific laboratory for detection, and various occasions for qualitative detection of the manual honey in actual work can be met.

Description

technical field [0001] The invention relates to the field of detection, in particular to the field of detection of Manuka honey, and more specifically to a method for rapidly characterizing Manuka honey. Background technique [0002] Manuka honey is a precious honey species unique to New Zealand. It is brewed by bees collecting the nectar of a local shrub plant—Manuka (Leptospermum scoparium). What sets Manuka honey apart from other honeys is its powerful and unique antibacterial activity. Honey generally has antibacterial activity, which is caused by the high permeability of honey itself, strong acidity and generally containing peroxides. The antibacterial activity of manuka honey is not dependent on peroxide, so it is called non-peroxide antibacterial activity (NPA). Medical research has shown that the superior antibacterial activity of Manuka honey has demonstrated its unique medicinal value in preventing wound infection, promoting wound healing, and treating foot ulcer...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/64
Inventor 王雪婷刘芸王艳姚燕林王恒丁涛王毅谦
Owner 南京海关动植物与食品检测中心
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