Rapid cerasus humilis tissue culture method
A technology of tissue culture and eucalyptus, applied in the field of plant tissue culture, can solve problems such as unsatisfactory reproduction efficiency of eucalyptus tissue and inability to meet demand and so on.
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[0050] Example 1
[0051] A rapid method for tissue culture
[0052] 1. Culture of explants
[0053] Remove the outer seed coat of the collected Oleander seeds, transfer them to a sterilized container on an ultra-clean workbench, disinfect with 70% alcohol for 10s, rinse with sterile water for 3 times, and then disinfect with 0.1% mercuric chloride solution 5min, rinsed 5 times with sterile water. The sterilized seeds were inoculated on the pre-prepared MS medium (without any hormones), and cultivated under the conditions of 13 light / 11 dark, the light intensity was 2500Lx, the temperature was 25°C, and the humidity was 40%. . After 15 days, when the seeds germinated and the seedlings grew to about 2 cm, the seedlings were cut into segments of about 1.0 cm, and at least one axillary bud was retained in each segment, which was used as an explant to induce the proliferation of adventitious buds.
[0054] 2. Induction of adventitious buds
[0055] The obtained seedling segme...
Example Embodiment
[0062] Example 2
[0063] 1. Culture of explants
[0064] Remove the outer seed coat of the collected Oleander seeds, transfer them to a sterilized container on an ultra-clean workbench, sterilize with 75% alcohol for 10s, rinse with sterile water for 3 times, and then disinfect with 0.1% mercuric chloride 5min, rinsed 5 times with sterile water. The sterilized seeds were inoculated on the pre-prepared MS medium without any hormones, and cultivated under the conditions of 13 / 11h light, the light intensity was 2500Lx, the temperature was 24°C, and the humidity was 50%. After 15 days, when the seeds germinated and the seedlings grew to about 2 cm, the seedlings were cut into segments of about 1.0 cm, and at least one axillary bud was retained in each segment, which was used as an explant to induce the proliferation of adventitious buds.
[0065] 2. Induction of adventitious buds
[0066] Seedling segments were inoculated on pre-prepared proliferation medium. The medium formu...
Example Embodiment
[0073] Example 3
[0074] 1. Culture of explants
[0075] Remove the outer seed coat of the collected Oleander seeds, transfer them to a sterilized container on an ultra-clean workbench, sterilize with 75% alcohol for 10s, rinse with sterile water for 3 times, and then disinfect with 0.1% mercuric chloride 5min, rinsed 5 times with sterile water. The sterilized seeds were inoculated on the pre-prepared MS medium without any hormones, and cultivated under the conditions of 13 / 11h light, the light intensity was 2500Lx, the temperature was 26°C, and the humidity was 45%. After 15 days, when the seeds germinated and the seedlings grew to about 2 cm, the seedlings were cut into segments of about 1.0 cm, and at least one axillary bud was retained in each segment, which was used as an explant to induce the proliferation of adventitious buds.
[0076] 2. Induction of adventitious buds
[0077] Seedling segments were inoculated on pre-prepared proliferation medium. The medium formu...
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