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Rapid cerasus humilis tissue culture method

A technology of tissue culture and eucalyptus, applied in the field of plant tissue culture, can solve problems such as unsatisfactory reproduction efficiency of eucalyptus tissue and inability to meet demand and so on.

Pending Publication Date: 2021-07-13
INNER MONGOLIA AUTONOMOUS REGION ACAD OF FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this method is unsatisfactory to the propagation efficiency of Plum tissue culture, can not satisfy the demand of market to a large amount of Prunus plants

Method used

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  • Rapid cerasus humilis tissue culture method
  • Rapid cerasus humilis tissue culture method
  • Rapid cerasus humilis tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A kind of fast Oligo tissue culture method

[0052] 1. Culture of explants

[0053] Remove the outer testa of the collected Plum seeds, transfer them to a sterilized container on an ultra-clean workbench, disinfect with 70% alcohol for 10 seconds, and rinse with sterile water for 3 times; then disinfect with 0.1% mercuric chloride solution 5min, rinse with sterile water 5 times. Inoculate the sterilized seeds on the pre-prepared MS medium (without any hormones), cultivate under the condition of 13 light / 11 dark, the light intensity is 2500Lx, the temperature is 25°C, and the humidity is 40% for cultivation . After 15 days, when the seeds germinate and the seedlings grow to about 2 cm, the seedlings are cut into sections of about 1.0 cm, and each section retains at least one axillary bud, which is used as an explant to induce the proliferation of adventitious buds.

[0054] 2. Induction of adventitious buds

[0055] The obtained seedling segments were used as explant...

Embodiment 2

[0063] 1. Culture of explants

[0064] Remove the outer testa of the collected Plum seeds, transfer them to a sterilized container on an ultra-clean workbench, disinfect with 75% alcohol for 10 seconds, and rinse with sterile water for 3 times; then disinfect with 0.1% mercuric chloride solution 5min, rinse with sterile water 5 times. The sterilized seeds were inoculated on the pre-prepared MS medium without any hormone, and cultivated under the conditions of 13 / 11h light, light intensity of 2500Lx, temperature of 24°C, and humidity of 50%. After 15 days, when the seeds germinate and the seedlings grow to about 2 cm, the seedlings are cut into sections of about 1.0 cm, and each section retains at least one axillary bud, which is used as an explant to induce the proliferation of adventitious buds.

[0065] 2. Induction of adventitious buds

[0066] The seedling segments were inoculated on the pre-prepared proliferation medium. The medium formula is MS+6-BA1.0mg / L+NAA0.05mg / L...

Embodiment 3

[0074] 1. Culture of explants

[0075] Remove the outer testa of the collected Plum seeds, transfer them to a sterilized container on an ultra-clean workbench, disinfect with 75% alcohol for 10 seconds, and rinse with sterile water for 3 times; then disinfect with 0.1% mercuric chloride solution 5min, rinse with sterile water 5 times. The sterilized seeds were inoculated on the pre-prepared MS medium without any hormone, and cultivated under the conditions of 13 / 11h light, light intensity of 2500Lx, temperature of 26°C, and humidity of 45%. After 15 days, when the seeds germinate and the seedlings grow to about 2 cm, the seedlings are cut into sections of about 1.0 cm, and each section retains at least one axillary bud, which is used as an explant to induce the proliferation of adventitious buds.

[0076] 2. Induction of adventitious buds

[0077] The seedling segments were inoculated on the pre-prepared proliferation medium. The medium formula is MS+6-BA0.7mg / L+NAA0.05mg / L...

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Abstract

The invention provides a rapid cerasus humilis tissue culture method, and belongs to the technical field of plant tissue culture. The rapid cerasus humilis tissue culture method comprises the following steps that disinfected seeds are germinated to obtain cerasus humilis seedling segments, and at least one axillary bud is kept on each seedling segment; the seedling segments serving as cerasus humilis explants are inoculated to an adventitious bud induction culture medium for adventitious bud induction culture to obtain a cerasus humilis adventitious bud; the cerasus humilis adventitious buds are inoculated to a rooting culture medium for rooting culture, and cerasus humilis seedlings with adventitious roots are obtained; and the cerasus humilis seedlings with the adventitious roots are hardened and transplanted to obtain complete cerasus humilis plants. According to the rapid cerasus humilis tissue culture method, the seedlings obtained by seed germination are cut off to obtain the seedling segments with the axillary buds, and the seedling segments are used as the explants for tissue culture, so that the adventitious bud induction rate is greatly improved, the number of the cerasus humilis plants is further improved, and the requirements of the market on the cerasus humilis plants are met.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a rapid tissue culture method of Prunus aurantium. Background technique [0002] Ou Li is also called Shanmeizi, Xiaoliren, and Lier, etc. It is mainly distributed in Heilongjiang, Jilin, Liaoning, Inner Mongolia, Hebei, Shandong, Shanxi and other places in China. It is understood that the ingredients of Ou Li contain a variety of nutrients that are beneficial to the human body, especially the calcium content in it is much higher than that of other fruits. In fact, Oli is also a kind of Chinese medicinal material with diuresis and dampness. In the prior art, the domestication and cultivation of Prunus prunus is conducive to enriching the types of fruit varieties in the market. [0003] In the prior art, in order to realize the artificial cultivation of Prunus chinensis, various breeding techniques are adopted, such as seed planting and twig cutting seed...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 王美珍莎仁图雅乌日恒吴振廷赵丽李娜刘佳宁静
Owner INNER MONGOLIA AUTONOMOUS REGION ACAD OF FORESTRY SCI
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