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Culture medium for chrysanthemum morifolium petal callus induction and application

A technology of callus induction and callus, which is applied in the field of plant tissue culture, can solve the problems of low callus induction rate, slow callus growth, medium classification discussion, etc., and achieve the solution of petal callus induction rate Low, increase the callus weight coefficient, improve the effect of petal callus induction rate and weight coefficient

Inactive Publication Date: 2021-07-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing callus induction methods of chrysanthemum petals still have problems such as slow callus growth, low callus induction rate, and no classification and discussion of culture media according to genotypes.

Method used

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  • Culture medium for chrysanthemum morifolium petal callus induction and application
  • Culture medium for chrysanthemum morifolium petal callus induction and application
  • Culture medium for chrysanthemum morifolium petal callus induction and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Test materials: cut chrysanthemum 'Flame', 'Freig', 'Roman Holiday', 'September Nine'.

[0027] 2. Test medium: 4.43g / L MS+30g / L sucrose+3g / L plant gel+0.5-2.0mg / L 6-BA+0.5-2.0mg / L NAA, pH 5.8. Cut chrysanthemum petal callus induction medium 1, 2, 3, 4, hormone ratio as shown in Table 1.

[0028] 3. Test method:

[0029] Material disinfection and inoculation: take petals, put them in a beaker filled with warm water at 25°C, drop 3 drops of Tween-80, and let it stand for 30 minutes; seal the mouth of the cup with gauze, and rinse it under running water for 1 hour. Shake constantly to wash away the adherents on the surface of the material; on the ultra-clean workbench, use tweezers to transfer the petals into a sterilized beaker, add a 70% alcohol solution for 5 seconds, and then rinse with sterile water for 3 times; add 0.1 % mercuric chloride for 5 minutes, then rinsed with sterile water for 5 times; take out the sterilized petals, blot the water with sterilized fi...

Embodiment 2

[0044] 1. Test material: cut chrysanthemum 'girl powder'.

[0045] 2. Test medium: same as Example 1;

[0046] 3. Test method: with embodiment 1.

[0047] 4. Test results:

[0048] In this example, the callus induction rate of petals in medium No. 2 (4.43g / L MS+30g / L sucrose+3g / L plant gel+1.0mg / L 6-BA+2.0mg / L NAA) Both were 100%, and the weight of callus was the largest, which indicated that No. 2 medium was the most suitable medium for inducing callus from the petals of cut chrysanthemum 'Maiden Pink'.

[0049] Table 4 Effects of different hormone concentration ratios on the callus induction rate of different varieties of cut chrysanthemum

[0050] Medium 1 medium 2 medium 3 Medium 4 girl powder 66.67 100.00 93.33 86.67

[0051] Table 5 Effects of different hormone concentration ratios on callus weight coefficient of different varieties of cut chrysanthemum

[0052] Medium 1 medium 2 medium 3 Medium 4 girl powder 0.02...

Embodiment 3

[0054] 1. Test materials: cut chrysanthemum 'wilderness', 'redost yellow', 'frank powder', 'margit', 'red dante', 'country music'.

[0055] 2. Test medium: the same as in Example 1.

[0056] 3. Test method: with embodiment 1.

[0057] 4. Test results:

[0058] In this example, the callus induction rate of petals in medium No. 3 (4.43g / L MS+30g / L sucrose+3g / L plant gel+2.0mg / L 6-BA+0.5mg / L NAA) Both are 100%, and the weight of callus is the largest, indicating that No. 3 culture medium is cut flower chrysanthemum 'wilderness', 'Ridost yellow', 'frank powder', 'Margit', 'red Dante', 'country Optimum medium for callus induction of music' petals.

[0059] Table 6 Effects of different hormone concentration ratios on the callus induction rate of different varieties of cut chrysanthemum

[0060] Medium 1 medium 2 medium 3 Medium 4 wilderness 73.33 83.33 100.00 86.67 Redoster Huang 90.00 100.00 100.00 100.00 Candid powder 100.00 100.00 1...

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Abstract

The invention provides a culture medium for chrysanthemum morifolium petal callus induction and application. The culture medium is prepared from 6-benzyladenine, naphthylacetic acid, MS (Murashige and Skoog), saccharose and phytagel. According to the culture medium, a plant growth regulator with high cost performance is selected, and NAA and 6-BA with specific concentration are added into the culture medium, so that the prepared culture medium not only effectively increases the induction rate of the petal callus, but also increases the weight coefficient of the callus. The culture medium involves a wide range of chrysanthemum morifolium varieties, and different cut-flower chrysanthemum varieties are classified and discussed to screen out the optimal callus induction culture medium for each variety. According to the culture medium, the induction rate and the weight coefficient of the petal callus of chrysanthemum morifolium varieties can be remarkably increased, the problems that the induction rate of the petal callus is low and the growth of the callus is slow are well solved, a foundation is laid for the next step of callus differentiation, and a good transgenic material is also provided.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a medium for inducing callus of cut chrysanthemum petals and its application. Background technique [0002] Chrysanthemum (Chrysanthemum morifolium) is a perennial herb of the genus Chrysanthemum in the family Compositae. It is rich in varieties and diverse in color. It is one of the top ten famous flowers in China and one of the four major cut flowers in the world. Chrysanthemum is usually propagated by cuttings, but its propagation efficiency is low, and it is greatly restricted by climatic conditions. Some rare chrysanthemum varieties and new varieties with insufficient female parent sources are difficult to meet market demand. The adoption of tissue culture technology can significantly improve the multiplication efficiency, accelerate the multiplication speed, realize the large-scale production of chrysanthemums, and have extremely high economic and s...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 李方滕如萍刘晓芬向理理李亚静
Owner ZHEJIANG UNIV
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