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Screening and preparation method of tea degrading bacteria

A technology for degrading bacteria and tea, applied in biochemical equipment and methods, microorganisms, microorganisms, etc., can solve problems such as low extraction rate

Pending Publication Date: 2021-07-16
无锡市茶叶品种研究所有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, generally speaking, the extraction rate is still low, and further research is needed to optimize its extraction process.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Configuration of culture medium

[0024] Beef extract peptone medium: beef extract 0.5%, peptone 1%, NaCl seed medium: glucose 1%, peptone 0.1%, yeast extract powder 0.1%, deionized water 10ml, pH 7, sterilized at 115°C for 30min.

[0025] Fermentation medium: tea dregs 0.5g, peptone 0.1%, yeast extract powder 0.1%, tap water 10ml, pH 7, sterilized at 115°C for 30min.

[0026] 2. Primary screening of strains

[0027] (1) Activation of bacteria. Use a pipette gun to draw 4ml of the original bacterial liquid obtained from the spoiled waste of tea leaves, insert it into the sterile fermentation medium, and culture it in a constant temperature incubator at 37°C for 48 hours, shake the bottle every 12 hours, and observe the tea in the activated bacterial liquid Changes in slag.

[0028] (2) Dilute the plate. Dilute the activated bacteria solution by 10 -4 times, add 900ul sterile saline. Draw 100ul of the diluted bacterial solution into the center of the sterilized ...

Embodiment 2

[0038] 1. Configuration of culture medium

[0039] Beef extract peptone medium: beef extract 0.5%, peptone 1%, NaCl seed medium: glucose 1%, peptone 0.1%, yeast extract powder 0.1%, deionized water 10ml, pH 7, sterilized at 115°C for 30min.

[0040] Fermentation medium: tea dregs 0.8g, peptone 0.1%, yeast extract powder 0.1%, tap water 10ml, pH 7, sterilized at 115°C for 30min.

[0041] 2. Primary screening of strains

[0042] (1) Activation of bacteria. Use a pipette gun to draw 4ml of the original bacterial liquid obtained from the spoiled waste of tea leaves, insert it into the sterile fermentation medium, and culture it in a constant temperature incubator at 37°C for 48 hours, shake the bottle every 12 hours, and observe the tea in the activated bacterial liquid Changes in slag.

[0043] (2) Dilute the plate. Dilute the activated bacteria solution by 10 -4times, add 900ul sterile saline. Draw 100ul of the diluted bacterial solution into the center of the sterilized b...

Embodiment 3

[0053] 1. Configuration of culture medium

[0054] Beef extract peptone medium: beef extract 0.5%, peptone 1%, NaCl seed medium: glucose 1%, peptone 0.1%, yeast extract powder 0.1%, deionized water 10ml, pH 7, sterilized at 115°C for 30min.

[0055] Fermentation medium: tea dregs 1g, peptone 0.1%, yeast extract powder 0.1%, tap water 10ml, pH 7, sterilized at 115°C for 30min.

[0056] 2. Primary screening of strains

[0057] (1) Activation of bacteria. Use a pipette gun to draw 4ml of the original bacterial liquid obtained from the spoiled waste of tea leaves, insert it into the sterile fermentation medium, and culture it in a constant temperature incubator at 37°C for 48 hours, shake the bottle every 12 hours, and observe the tea in the activated bacterial liquid Changes in slag.

[0058] (2) Dilute the plate. Dilute the activated bacteria solution by 10 -4 times, add 900ul sterile saline. Draw 100ul of the diluted bacterial solution into the center of the sterilized be...

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Abstract

The invention belongs to the field of strain preparation, and particularly relates to a screening and preparation method of tea degrading bacteria. According to the method, a beef extract peptone culture medium is prepared from tea residues as a raw material, an original bacterial solution obtained from rotten waste residues of tea leaves is inoculated into a sterile fermentation culture medium, the fermented solution is transferred into a seed culture medium after strain propagation, then separating and primarily screening are performed, and single colonies on different flat plates are picked to be re-screened and purified, and pure bacteria CJ are obtained. The tea residues are degraded to be converted into poultry feed, and huge social significance and economic value can be brought.

Description

technical field [0001] The invention belongs to the field of strain preparation, and in particular relates to a screening and preparation method of tea-degrading bacteria. [0002] technical background [0003] As one of the birthplaces of tea, my country is undoubtedly a big country in tea production. At present, the production of tea has become the first in the world. In 2017, my country's dry raw tea production has reached 2.58 million tons, and it still maintains a growing trend year by year. So much tea production also means a lot of tea by-products - tea dregs. [0004] Tea has a variety of high-content health care and medicinal ingredients, such as tea polyphenols, tea polysaccharides, theaflavins, caffeine, theanine, etc., and is one of the most valuable plant materials for development. There is a large amount of by-products of tea consumption in my country every year - tea dregs. Discarding them will not only pollute the environment, but also lead to a waste of res...

Claims

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Application Information

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IPC IPC(8): C12N1/00
CPCC12N1/00
Inventor 徐琪
Owner 无锡市茶叶品种研究所有限公司
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