Method for regenerating functional retinal ganglion cells by using transcription factor

A retinal ganglion and transcription factor technology, applied in the field of biomedicine, to achieve the effect of easy promotion

Pending Publication Date: 2021-07-20
SHANGHAI TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although people have done a lot of work on the regeneration of retinal ganglion cells, there is no case of successfully regenerating functional retinal ganglion cells in adult mammals. The present invention is the first breakthrough in this field

Method used

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  • Method for regenerating functional retinal ganglion cells by using transcription factor
  • Method for regenerating functional retinal ganglion cells by using transcription factor
  • Method for regenerating functional retinal ganglion cells by using transcription factor

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preparation example Construction

[0098] The preparation method of retinal ganglion cells in one embodiment of the present invention at least includes the following steps: applying transcription factors to adult cells to transform them into retinal ganglion cells; the transcription factors are selected from Brn3B, Sox4, Atoh7, Sox11 and Isl1 any one or more of.

[0099] In the present invention, unless otherwise specified, the pharmaceutical dosage form is not particularly limited, and can be made into dosage forms such as injections, oral liquids, tablets, capsules, dripping pills, sprays, etc., and can be prepared by conventional methods. The choice of drug dosage form should match the mode of administration.

[0100] The effective amount of the pharmaceutical preparation of the present invention should take into account factors such as the route of administration, the health status of the patient, etc., which are within the skill of skilled physicians.

[0101] Further, the adult cells are selected from on...

Embodiment 1

[0134] 1.1 Mice and feeding methods:

[0135] Lgr5 used in the present invention EGFP-IRES-CreERT2 , Pvalb CreERT2 , and the Rosa26-tdTomato mouse strain were purchased from Jackson laboratory. Lgr5 EGFP-IRES-CreERT2 Strain mice and Pvalb CreERT2 Strain mice were crossed with Rosa26-tdTomato mice to obtain Lgr5 EGFP-IRES-CreERT2 ; Rosa26-tdTomato mice and Pvalb CreERT2 ; Rosa26-tdTomato mice.

[0136] Prokr2 CreERT2 The mouse strain was constructed in the laboratory of ShanghaiTech University by using CRISPR / Cas9 technology through homologous recombination. The mouse was knocked into the CreERT2-PolyA expression box at the ATG site of the Prokr2 gene. The brief process of mouse construction is as follows: a vector (donor vector) containing CreERT2-PolyA expression cassette and homologous recombination arm was constructed by In-Fusion cloning method. Cas9 mRNA, gRNA and donor vector were microinjected into fertilized eggs of C57BL / 6J mice, and the injected fertilized eg...

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Abstract

The invention belongs to the field of biological medicine research, and particularly provides application of a transcription factor in one or more of the following items: preparing a medicine for treating retinal diseases; preparing a retina ganglion cell regeneration product; preparing a product for inducing adult cells to be reprogrammed into retinal ganglion cells; and preparing retinal ganglion cells. The transcription factor is selected from any one or more of Brn3B, Sox4, Atoh7, Sox11 and Isl1. A cell material used for regenerating the retinal ganglion cell is an endogenous cell of the retina ganglion cell, and compared with a cell transplantation mode, the retina ganglion cell has no immunological rejection and tumor formation risk; and the virus expression gene is simple, convenient and easy to popularize.

Description

technical field [0001] The invention belongs to the field of biomedical research, and in particular relates to a method for regenerating functional retinal ganglion cells by using transcription factors. Background technique [0002] Retinal ganglion cells are the only neurons in the retina that transmit nerve signals to the brain, and its disease and death are one of the main causes of blindness. Diseases associated with retinal ganglion cell lesions are very common in clinical practice, mainly including glaucoma, hereditary optic neuropathy, etc. [0003] According to the latest statistics, glaucoma alone has 79.6 million patients worldwide, of whom 11.2 million may eventually develop binocular blindness. According to epidemiological data in my country, the prevalence of glaucoma among people over 40 years old in China is 2.3%, and the blindness rate is about 30%. It is estimated that by 2020, there will be 21 million glaucoma patients in my country, and 6.3 million peopl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61K35/30A61P27/02A61P27/06C12N5/10C12N15/864
CPCA61K38/1709A61K35/30A61P27/02A61P27/06C12N5/0621C12N15/86C12N2506/08C12N2501/60C12N2510/00C12N2750/14143A01K67/0278A01K2217/072A01K2227/105A01K2267/0393A01K67/0275A61K48/005A61K48/0075C12N5/062C12N2501/602C12N2750/14023C12N2750/14133C12N2750/14142
Inventor 刘洪军未小虎张振豪乔娜刘璐
Owner SHANGHAI TECH UNIV
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