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Antibodies directed to filamin-a and therapeutic uses thereof

A filamin and antibody technology, applied in the direction of antibodies, immunoglobulins, hybrid immunoglobulins, etc.

Active Publication Date: 2021-07-23
IBEX BIOSCI LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Current approaches to treating cancer are fairly non-selective

Method used

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  • Antibodies directed to filamin-a and therapeutic uses thereof
  • Antibodies directed to filamin-a and therapeutic uses thereof
  • Antibodies directed to filamin-a and therapeutic uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0313] Example 1: Preparation of Human Chimeric Filamin-A Antibody

[0314] AHO1402 (a monoclonal mouse filamin-A IgGIk antibody) was obtained from Life Technologies as a solution in 95% BSA and 5% antibody. To remove BSA and purify antibodies, antibodies were separated and purified by gel electrophoresis, separated by size, and excised from the gel.

[0315] Gel slices containing antibodies were de novo sequenced using Database Assisted Shotgun Sequencing (DASS) technology, which utilizes high-resolution MS / MS peptide determination to obtain sequence information, followed by overlapping peptide sequence analysis. Compile to reconstruct the sequence. The DASS method is generally described on the Creative Biolabs website (http: / / www.creative-biolabs.com / next-generation-antibody-sequencing.html).

[0316] The constructed amino acid sequences of the light and heavy chain variable domains were then added to the murine and human constant region sequences chosen by the inventors. ...

Embodiment 2

[0318] Example 2: scFv preparation and characterization

[0319] The heavy and light chain variable regions were subcloned into scFv vector systems to generate scFv in two possible orientations, light-heavy (VL-VH or vl-vh) and heavy-light (VH-VL or vh- vl).

[0320] The resulting single chain antibodies will be tested in assays including immunofluorescence staining of cells, cell motility, proliferation, apoptosis and other assays to assess functional biological activity.

[0321] The scFvs will be further evaluated for use in diagnostic assays and methods of treating diseases such as breast cancer.

Embodiment 3

[0322] Example 3: Determination of cell motility

[0323] Cell motility assays were used to explore the functional activity of the mAbs generated in this disclosure (mouse antibody B186, human chimeric antibody B185 and human chimeric antibody B411).

[0324] Evidence of activity was obtained with murine and human chimeric antibodies. Surprisingly, however, human chimeric mAbs showed greater biological activity than murine mAbs. The effect was also more pronounced on fibronectin-coated tissue culture surfaces compared to collagen-coated surfaces.

[0325] Cell motility assay utilizing Platypus Technologies ORIS TM Cell migration assay platform, which is generally described at http: / / www.platypustech.com / discoverAssay.html.

[0326] This measure of motility, including 3D iterations, is thought to correlate with cancer cell invasiveness and malignancy, and is therefore a surrogate for cancer metastasis and other viability.

[0327] Specifically, in ORIS TM Filamin-A antibod...

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Abstract

The disclosure teaches antibodies that are useful, inter alia, in methods for detecting and treating human cancer. In a particular aspect, the disclosure teaches novel antibodies that are useful for detecting and treating human breast cancer. In some embodiments, the disclosure teaches novel antibodies that bind to filamin A. In some embodiments, the antibodies are intrabodies.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of U.S. Provisional Application No. 62 / 743,169, filed October 9, 2018, the entire contents of which are hereby incorporated by reference. [0003] Description of electronically submitted text files [0004] The contents of the text file accompanying the electronic submission are hereby incorporated by reference in their entirety: Copy of Sequence Listing in Computer Readable Form (File Name: IBEX_004_03WO_ST25.txt: Date of Record: October 9, 2019; File Size: 4 kilobytes ). technical field [0005] The present disclosure relates to monoclonal antibodies (mAbs) and fragments thereof, novel protein expressing cell lines that secrete said antibodies or fragments thereof, and the use of said antibodies and antibody fragments for preferential detection of antigens and / or treatment of disease. In certain embodiments, the antibodies and fragments thereof provided herein modulate cell meta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K51/10C07K16/30C07K16/46
CPCC07K16/30C07K16/18C07K2317/24C07K2317/70C07K2317/80C07K2317/622C07K2317/73A61K2039/86A61P35/00A61K2039/80A61K2039/884A61K2039/812A61K51/10A61K51/1093A61K47/62G01N33/57415G01N33/6854C07K16/2809C07K2317/565C07K2317/92A61K2039/505C07K2317/33G01N2333/4712
Inventor M·J·卡林A·M·克罗西V·F·德拉科鲁兹N·Z·莱R·费森
Owner IBEX BIOSCI LLC
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