Separation and detection method of seaweed kelose in stingless bee honey
A trehalulose and detection method technology, which is applied in the field of detection of active ingredients in honey, can solve the problems of trehalulose detection method, etc., and achieve the effect of simple and accurate detection
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Embodiment 1
[0026] This embodiment relates to a separation method of a non-honey honey, comprising the steps of:
[0027] 1) Treatment of yellow-shaped non-honey as follows: Accurately weigh 0.5 g from the beaker, add 5 mL of water to dissolve, transferred to a 10 mL volumetric flask, use 3 ml water to wash the cup, and transfer to the same In the capacity bottle, pure water is set. Take 1 ml of sample solution with a disposable syringe over 0.22 μm nylon filters to injectable vials, ready to injection;
[0028] 2) Controlling liquid chromatographic detection conditions: column: Hi-PLEX PB, 300 × 7.7 mm, 8 μm; mobile phase: pure water; column temperature: 50 ° C; pressure: 0.6 kPa, injection detection (separation results like figure 1 ).
Embodiment 2
[0030] This embodiment relates to a separation method of a non-honey honey, comprising the steps of:
[0031] 1) Treatment of the top-free bee honey as follows: Accurately weigh 0.5 g from the beaker, add 5 mL of water to dissolve, transferred to a 10 mL volume bottle, washed with 3 mL water to wash the cup, and transferred to the same In the capacity bottle, pure water is arranged. Take 1 ml of sample solution with a disposable syringe over 0.22 μm nylon filters to injectable vials, ready to injection;
[0032] 2) Controlling liquid chromatographic detection conditions: column: Hi-PLEX PB, 300 × 7.7 mm, 8 μm; mobile phase: pure water; column temperature: 50 ° C; pressure: 0.6 kPa, injection detection (separation results like figure 2 ).
Embodiment 3
[0034] This embodiment relates to a separation method of a non-honey honey, comprising the steps of:
[0035] 1) Treatment of the Gobs free honey: accurately weigh 0.5 g of sample in the beaker, add 5 ml of water to dissolve, transferred to a 10 mL capacity bottle, use 3 ml water to wash the cup, and transfer to the same In the capacity bottle, pure water is set. Take 1 ml of sample solution with a disposable syringe over 0.22 μm nylon filters to injectable vials, ready to injection;
[0036] 2) Controlling liquid chromatographic detection conditions: column: Hi-PLEX PB, 300 × 7.7 mm, 8 μm; mobile phase: pure water; column temperature: 50 ° C; pressure: 0.6 kPa, injection detection (separation results like image 3 ).
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