Method for detecting cleanliness of cold-chain food outer package based on ATP

Abstract

The invention discloses a method for detecting the cleanliness of a cold-chain food outer package based on ATP (adenosine triphosphate) which comprises the following steps: step 1, taking a plurality of ATP swabs, in every two ATP swabs, filling an ATP enzyme solution and a reaction solution into one ATP swab, and filling an ATP enzyme solution, a selective bacterial culture solution and a reaction solution into the other ATP swab; 2, putting a plurality of ATP swabs into the tube seat, and enabling a plurality of ejector pins on the tube seat to extend into the swabs respectively; 3, sampling the two sampling tubes filled with different reagents in parallel; 4-6, pushing the push plate downwards to enable the sample to be in contact with the ATP enzyme solution, the selective bacterial culture solution and the reaction solution in sequence; and 7, performing detecting and comparing to obtain the cleanliness of the outer package of the cold-chain food. The method has the characteristics of simple multiple detection operations, accurate and comprehensive detection and capability of detecting specific bacteria.

Description

technical field [0001] The invention relates to a method for detecting cleanliness, in particular to an ATP-based method for detecting the cleanliness of outer packaging of cold-chain foods. Background technique [0002] Adenosine triphosphate (ATP) is the direct source of energy for all living organisms. It is ubiquitous in animal and plant cells, microorganisms, and food residues. The ATP fluorescence detection method is a rapid detection technology developed based on the principle of firefly luminescence. Luciferase can catalyze luciferin. It reacts with ATP to form fluorescein and emits fluorescence. The intensity of fluorescence emitted is proportional to the number of microorganisms. By testing the intensity of fluorescence, the degree of microbial contamination of the test object can be known. Therefore, detection of ATP can be used to judge cold chain food A visual indicator of whether the outer packaging is clean. [0003] Existing ATP detection methods generally u...

AI Technical Summary

Problems solved by technology

[0003] Existing ATP detection methods generally use ATP swabs directly on the surface of the object to be sampled and detected. The detection range is narrow at one time, and the detection data is not comprehensive enough. If multiple detections are required, multiple tests and reactions are required. , the operation is troublesome and time-consuming; and in the sampled sample, in addition to the ATP in the bacteria, there are some free ATP, which will interfere with the detection data of the actual bacteria and affect the accuracy of the detection; and after the reaction, only the total The amount of bacteria, it is impossible to know whether there are some special pathogenic bacteria

[0004] Therefore, the existing ATP detection method has the characteristics of troublesome multiple detection operations, detection accuracy to be improved, and inability to detect specific bacteria.

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