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Prostate cancer PDX model construction method

A prostate cancer and construction method technology, applied in the field of experimental animal models, can solve problems such as obstacles to clinical efficacy prediction and preclinical research, limited types, etc., and achieve the effect of overcoming limited animal models, low cost, and high tumor formation rate

Pending Publication Date: 2021-08-03
博济医药科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the types of solid tumor animal models are limited, which cannot meet the needs of preclinical research and hinder the prediction of clinical efficacy and preclinical research. Therefore, it is necessary to establish an efficient and stable tumor PDX model

Method used

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  • Prostate cancer PDX model construction method
  • Prostate cancer PDX model construction method
  • Prostate cancer PDX model construction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1. A method for constructing a PDX model of prostate cancer

[0025] A method for constructing a prostate cancer PDX model, comprising the following steps:

[0026] 1) Cell extraction: Take a healthy B-NOD mouse, make a T-shaped incision on the lower abdomen, extract the seminal vesicles, peel off the superficial fascia, gently grind on a 200-mesh sieve, collect seminal vesicle cells, and mix them with a small amount of DMEM to obtain For seminal vesicle cell suspension, adjust the proportion of seminal vesicle cells in seminal vesicle cell suspension to 2×10 6 Individual / mL concentration;

[0027] 2) Resuscitate the animal tissue block and shred it to a size of 10-30mm 3 , mixing the shredded animal tissue block and seminal vesicle cell suspension at a volume ratio of 1:1 to obtain a tissue block containing seminal vesicle cells;

[0028] 3) Move the tissue block containing the seminal vesicle gland cells to a culture dish containing EPS to obtain a mixed so...

Embodiment 2

[0031] Example 2. A method for constructing a PDX model of prostate cancer

[0032] A method for constructing a prostate cancer PDX model, comprising the following steps:

[0033] 1) Cell extraction: Take a healthy B-NOD mouse, make a T-shaped incision on the lower abdomen, extract the seminal vesicles, peel off the superficial fascia, gently grind on a 200-mesh sieve, collect seminal vesicle cells, and mix them with a small amount of DMEM to obtain For seminal vesicle cell suspension, adjust the proportion of seminal vesicle cells in seminal vesicle cell suspension to 3×10 6 Individual / mL concentration;

[0034] 2) Resuscitate the animal tissue block and shred it to a size of 10-30mm 3 , mixing the shredded animal tissue block and seminal vesicle cell suspension at a volume ratio of 1:1 to obtain a tissue block containing seminal vesicle cells;

[0035] 3) Move the tissue block containing the seminal vesicle gland cells to a culture dish containing EPS to obtain a mixed so...

Embodiment 3

[0038] Example 3. A method for constructing a PDX model of prostate cancer

[0039] A method for constructing a prostate cancer PDX model, comprising the following steps:

[0040] 1) Cell extraction: Take a healthy B-NOD mouse, make a T-shaped incision on the lower abdomen, extract the seminal vesicles, peel off the superficial fascia, gently grind on a 200-mesh sieve, collect seminal vesicle cells, and mix them with a small amount of DMEM to obtain For seminal vesicle cell suspension, adjust the proportion of seminal vesicle cells in seminal vesicle cell suspension to 4×10 6 Individual / mL concentration;

[0041] 2) Resuscitate the animal tissue block and shred it to a size of 10-30mm 3 , mixing the shredded animal tissue block and seminal vesicle cell suspension at a volume ratio of 1:1 to obtain a tissue block containing seminal vesicle cells;

[0042] 3) Move the tissue block containing the seminal vesicle gland cells to a culture dish containing EPS to obtain a mixed so...

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Abstract

The invention relates to the technical field of experimental animal models, and particularly discloses a prostate cancer PDX model construction method. The construction method comprises the following steps: uniformly mixing animal tissue blocks which are cut into pieces with seminal vesicle cell suspension with the cell proportion of 2*10<6>-5*10<6> / mL to obtain a mixed solution, then mixing the mixed solution with matrigel, transplanting the mixed solution and matrigel into an animal body, and transplanting a tumor cell solution into the animal body for passage. The prostate cancer PDX model obtained with the construction method has high similarity with clinical prostate cancer, the tumor formation rate is high after tumor cell solution transplantation, the original microenvironment of tumor tissue is reserved, the operation method is simple, the cost is low, and the method can be widely applied to individualized precise diagnosis and treatment of prostatic cancer, preclinical drug screening of new anti-cancer drugs and research of prostatic cancer.

Description

technical field [0001] The invention relates to the technical field of experimental animal models, in particular to a method for constructing a prostate cancer PDX model. Background technique [0002] Tumor is the most serious disease that endangers human health and is one of the leading causes of human death. According to statistics from the World Health Organization, there were about 12.7 million new cancer cases and 7.6 million cancer deaths in the world in 2008. At present, there are about 9 million new cancer patients in the world every year. It is estimated that by 2025, the incidence of cancer worldwide will increase by about 60%, and the number of new cancer patients in the world will reach 15 million every year. The morbidity and mortality rate of cancer in my country ranks first in the world. The number of people who die from cancer every year exceeds 1.6 million, and the incidence of cancer is about 2 million per 100,000 people. One of them died of tumor, ranking...

Claims

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Application Information

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IPC IPC(8): A01K67/027
CPCA01K67/0271A01K2207/12A01K2227/105A01K2267/0331
Inventor 马仁强严科池王廷春
Owner 博济医药科技股份有限公司
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