Application of silymarin in preparation of drugs for preventing and/or treating neuronal injury after cerebral ischemia

A technology for neuron damage and silymarin, which is applied in the application field of silibinin in the preparation of drugs for preventing and/or treating neuron damage after cerebral ischemia, can solve the problems of no report on the preventive effect and the like, and achieves the Effects of treating neuronal damage and death, inhibiting neuronal damage

Inactive Publication Date: 2021-08-03
葛鹏飞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Up to now, there has been no report on the prevention and treatment effect of Silydianin on neurological diseases, especially on neuron damage and death after cerebral ischemia

Method used

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  • Application of silymarin in preparation of drugs for preventing and/or treating neuronal injury after cerebral ischemia
  • Application of silymarin in preparation of drugs for preventing and/or treating neuronal injury after cerebral ischemia
  • Application of silymarin in preparation of drugs for preventing and/or treating neuronal injury after cerebral ischemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Silyjinin inhibits the killing effect of oxygen-glucose deprivation (OGD) on human SH-SY5Y cells

[0021] Silyjining (purchased from Chengdu Ruifensi Company) was formulated into a mother solution with DMSO during use, and administered after dilution.

[0022] Human SH-SY5Y cells (purchased from China Jikai Biotechnology Co., Ltd.), using oxygen-glucose deprivation (OGD) to make a cell model of "neuron injury and death after cerebral ischemia", LDH (lactate dehydrogenase) release method to detect cell injury and death.

[0023] Human SH-SY5Y cells were inoculated in DMEM medium in 96-well plates, incubated overnight in a carbon dioxide incubator, and incubated with different concentrations of silymarin (2.0mmol / L and 4.0mmol / L) 1 hour in advance, and oxygen After 24 hours of sugar deprivation, the release degree of lactate dehydrogenase (LDH) was detected.

[0024] Experimental results such as figure 1 shown. The results showed that the OD values ​​of LDH ...

Embodiment 2

[0032] Example 2 Silyjinin inhibits AIF nuclear translocation in human SH-SY5Y cells induced by oxygen-glucose deprivation (OGD)

[0033] The release of AIF (Apoptosis inducing factor) from mitochondria and into the nucleus is one of the main causes of programmed cell death. In order to elucidate the inhibitory effect of silymarin on the death of human SH-SY5Y cells induced by oxygen-glucose deprivation (OGD), in this experiment, human SH-SY5Y cells were inoculated in DMEM medium in a culture dish and incubated overnight in a carbon dioxide incubator. Incubate with different concentrations of silymarin (2.0mmol / L and 4.0mmol / L) 1 hour in advance. After 24 hours of oxygen-glucose deprivation, the cells are collected, and after lysis, the mitochondrial components and nuclei are separated by differential centrifugation. Then, Western blot analysis was used to detect the effect of silymarin on the nuclear translocation of AIF induced by oxygen-glucose deprivation (OGD).

[0034] ...

Embodiment 3

[0035] Example 3 Silyjinin inhibits oxygen-glucose deprivation (OGD)-induced DNA double-strand scission in human SH-SY5Y cells

[0036] DNA is the carrier of genetic information, and DNA double-strand breaks are the basis for DNA dissolution and cell death. In order to illustrate the protective effect of silymarin on cells, this experiment detected the effect of silymarin on DNA double-strand breaks in human SH-SY5Y cells induced by oxygen-glucose deprivation (OGD) by Western blot.

[0037] Experimental results such as image 3 shown. The results showed that oxygen-glucose deprivation (OGD) could significantly up-regulate the protein level of γ-H2AX, a marker of DNA double-strand breaks, but silymarin inhibited γ-H2AX induced by oxygen-glucose deprivation (OGD) in a concentration-dependent manner. expression up-regulated. This shows that silymarin can inhibit DNA double-strand damage caused by oxygen-glucose deprivation (OGD) in human SH-SY5Y cells.

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Abstract

The invention provides the application of silymarin in preparation of drugs for preventing and/or treating neuronal injury after cerebral ischemia and relates to the technical field of prevention and treatment of cerebral ischemia, experiments prove that silymarin can inhibit human SH-SY5Y cell injury and death induced by oxygen-glucose deprivation, and silymarin can significantly inhibit AIF nuclear translocation induced by oxygen-glucose deprivation, so that silymarin can be used for preparing drugs for preventing and/or treating neuronal injury after cerebral ischemia. Silymarin inhibits expression up-regulation of gamma-H2AX induced by oxygen-glucose deprivation in a concentration-dependent manner, can inhibit DNA double-chain damage of human SH-SY5Y cells caused by oxygen-glucose deprivation, and inhibits quantity up-regulation of proteins labeled by oxygen-glucose deprivation induced Ubiquitin in a concentration-dependent manner. Silymarin can inhibit protein aggregation caused by oxygen-glucose deprivation. The invention develops a new application of silymarin, and provides a new prevention and treatment way and a new prevention and treatment method for preventing and treating common nervous system diseases which are cerebral ischemia and influence the life quality of old people.

Description

technical field [0001] The invention relates to the technical field of prevention and treatment of cerebral ischemia, in particular to the application of silymarin in the preparation of drugs for preventing and / or treating neuron damage after cerebral ischemia. Background technique [0002] Silydianin is a flavonoid monomer small molecular compound extracted from the fruit of the genus Silybum marianum (Milk thistle) in the family Asteraceae. Molecular formula: C 25 h 22 o 10 , Molecular weight 482, white needle crystal. CAS number: 29782-68-1. It has the characteristics of convenient material collection and low price. [0003] Cerebral ischemia refers to insufficient blood supply to the brain, which can be caused by a variety of diseases, including cerebrovascular arteriosclerosis, cerebral vasospasm, and compression of the vertebrobasilar artery. Cerebral ischemia includes two types: transient ischemic attack and cerebral infarction. If cerebral ischemia lasts for a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/357A61K36/28A61P9/10A61P25/00
CPCA61K31/357A61K36/28A61P9/10A61P25/00
Inventor 葛鹏飞罗天飞孟繁凯
Owner 葛鹏飞
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