Method and device for detecting HIV-1 P24 antigen based on silicon nitride (SiNx) solid nanopore

Abstract

The invention discloses a method and a device for detecting an HIV-1P24 antigen based on a silicon nitride (SiNx) solid nanopore, and the method comprises the following steps: S1, a pretreatment step: carrying out pretreatment on a SiNx film chip with a window before use; s2, preparing a nanopore, namely preparing the required nanopore in the SiNx thin film chip by using a multistage current pulse breakdown method; s3, detecting an ion blocking current pulse signal generated by the nanopore in the sample; and S4, analyzing, detecting and calculating the detection limit of the sample. According to the present invention, the HIV-1P24 antigen molecule can be well detected, the spatial structure change of the sample during the translocation can be inferred, and the HIV-1P24 antigen molecule at the low concentration can be detected. The method and the device have the advantages of rapidness, detection timeliness, high sensitivity, high signal-to-noise ratio and high flux, further shorten the window period of HIV detection, and strive for earlier diagnosis opportunities for infected persons.

Description

technical field [0001] The invention belongs to the technical field of antigen detection and analysis, and relates to a method and device for detecting HIV-1 P24 antigen based on a silicon nitride (SiNx) solid-state nanopore, which can be applied to the field of protein analysis and detection. Background technique [0002] HIV is a virus that attacks the immune system and is also the pathogen of AIDS (Acquired Immune Deficiency Syndrome, AIDS). Therefore, it is very important for HIV-infected patients to diagnose early, target treatment, and prolong life. [0003] Nanopore single-molecule detection technology originated from the invention of the Coulter counter and the recording technology of single-channel current. It is a single-molecule analysis method developed in 1996. It is a non-labeling, real-time monitoring, high sensitivity, fast detection speed and operation. Simple and other advantages of new sensing detection technology. Nanopore single-molecule detection techn...

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Problems solved by technology

[0004] So far, HIV laboratory diagnosis is mainly based on serological tests for primary screening and confirmation tests. The first-generation primary screening reagents are prone to false positive and false negative diagnoses when detecting antibodies in serum; the second-generation primary screening reagents are simultaneously Detect HIV-1 and HIV-2, but it is easy to miss; the third-generation primary screening reagents use double-antigen detection antibodies, which are more sensitive than before but have a longer window period; the fourth-generation primary screening reagents shorten the window period by 4~ 7 days, but still need a window period of about 2 weeks

Although the detection of HIV nucleic acid sequence alone can shorten the window period, it requires professional testing, and the steps are cumbersome and expensive, which is not conducive to the popularization of hospitals

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