Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Substrate for nucleic acid analysis, flow cell for nucleic acid analysis, and image analysis method

A technology of nucleic acid analysis and analysis method, which is applied in the field of spot part configuration, can solve problems such as difficult identification of the position of nucleic acid attached spots, and achieve the effect of improving accuracy and processing speed

Pending Publication Date: 2021-08-06
HITACHI HIGH-TECH CORP
View PDF23 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Even if the patterned spots like the above-mentioned Patent Document 1 are formed and the density is increased, since the attached spots are periodically arranged, it is difficult to identify the attached spots of nucleic acid in the case of a positional shift due to erroneous recognition. Location

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Substrate for nucleic acid analysis, flow cell for nucleic acid analysis, and image analysis method
  • Substrate for nucleic acid analysis, flow cell for nucleic acid analysis, and image analysis method
  • Substrate for nucleic acid analysis, flow cell for nucleic acid analysis, and image analysis method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] use Figure 7 An example of a substrate for nucleic acid analysis including a patterned spot portion and a random spot portion on the surface of the substrate will be described.

[0059] Figure 7 It is an enlarged view of a part on the substrate. On the substrate, there are a patterned spot portion 701 , which is a region where nucleic acid attachment spots are regularly arranged, and a random spot portion 702 , which is a region where nucleic acid adheres irregularly. exist Image 6 In -A, the part where the circular parts are arranged represents the pattern-shaped spot part 701, and the circular parts represent the adhesion spots to which the sample adheres. Furthermore, the triangular part is a random spot part 702 . Each spot part has a nucleic acid-attached region formed of an amino group-containing coating film, and the surface of the non-nucleic acid-attached region is coated with hydrophobic HMDS. In the pattern-like spots, nucleic acid adheres to the arra...

Embodiment 2

[0066] An example of an image acquisition and alignment method using a nucleic acid analysis substrate having a patterned spot portion and a random spot portion will be described.

[0067] A nucleic acid sample to be analyzed is immobilized on a patterned spot portion and a random spot portion of a substrate disposed on the flow cell. Then, nucleotides with fluorescent substances are introduced by extension reaction, and four kinds of fluorescent images corresponding to four kinds of DNA bases are photographed and obtained. In each cycle of one base extension, four kinds of fluorescent images were observed as bright spots per field of view. exist Figure 9 , four examples of fluorescence images are shown. White circles indicate bright spots. Bright spots can be detected as bright spots on the fluorescence image. The bright spot positions of the image 905 obtained by combining the images ( 901 , 902 , 903 , 904 ) corresponding to the four types of A, T, G, and C indicate th...

Embodiment 3

[0085] Figure 15 An example of an image alignment method is shown.

[0086] A reference image 1501 is created based on the design information of the board. For example, the reference image can be created by simulation or the like. Here, the reference image refers to an image of the position of the spot as a reference used to align the position coordinates of the spot on the fluorescent image serving as the bright spot. When the alignment is performed only by the positional information of the spots, the image does not need to be created. The reference image can be prepared in advance according to the substrate used. For the pre-created reference image, the reference image can also be retrieved from the storage medium according to the substrate used. The first reference image created from the design information of the substrate is the position of the adhering speckles of the patterned speckle portion. Depending on the conditions of use, there may be information on the area...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
sizeaaaaaaaaaa
Login to View More

Abstract

At the positions of spots which are arranged on a substrate, image aligning is made difficult by the occurrence of a recognition error of the positions of spots, said spots being adjacent to each other in a patterned form, or a displacement caused by the expansion or deformation of the substrate due to device operation, temperature control, etc. The present invention provides: a substrate for nucleic acid analysis, on the surface of which a patterned spot area provided with spots to which a biopolymer is adhered and a randomly distributed spot area are formed; and an analysis method.

Description

technical field [0001] The present invention relates to a substrate for nucleic acid analysis, a flow cell for nucleic acid analysis, and an image alignment method, and relates to the arrangement of patterned spots and random spots for analysis of biological-related substances. Background technique [0002] In recent years, in nucleic acid analyzers, a large amount of base sequence information can be sequenced simultaneously and in parallel. The nucleic acid to be analyzed is immobilized on the substrate, and the sequencing reaction is repeated. A technique is used in which a fluorescent nucleotide for determining a base is introduced into the base sequence of a nucleic acid, and the base is determined from a fluorescent spot emitted therefrom. Images corresponding to a plurality of bases of nucleic acid are respectively provided from the apparatus. In a sequencing unit called 1 cycle, the amount of 1 base is sequenced in each fixed nucleic acid. By repeating this cycle, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12Q1/6874G01N33/53G01N37/00
CPCC12Q1/6874G01N33/543C12M1/00G01N33/53G01N37/00G06T7/32G06T7/337G06T7/0012G06T2207/30072
Inventor 马场纪子奈良原正俊板桥直志横山彻
Owner HITACHI HIGH-TECH CORP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com