Primer and probe composition and real-time fluorescent quantitative PCR (polymerase chain reaction) kit for para-influenza virus typing detection

A real-time fluorescence quantitative and virus typing technology, applied in the field of biomedicine, can solve the problems of poor primer specificity, low accuracy of detection results, poor primer sensitivity, etc.
CN113278738APending Publication Date: 2021-08-20NINGBO HEALTH GENE TECHNOLOGIES CO LTD +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NINGBO HEALTH GENE TECHNOLOGIES CO LTD
Publication Date
2021-08-20

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Abstract

The invention discloses a primer and probe composition and real-time fluorescent quantitative PCR (polymerase chain reaction) kit for para-influenza virus typing detection, which can be used for detecting at least two of para-influenza virus type 1, para-influenza virus type 2, para-influenza virus type 3 and para-influenza virus type 4. In the primer and probe composition, four groups of primers and probes all have excellent specificity and can specifically perform typing detection on corresponding parainfluenza virus serotypes, the first group of primers and probes can specifically amplify a parainfluenza virus type 1 HN gene, the second group of primers and probes can specifically amplify a parainfluenza virus type 2 L gene, the third group of primers and probes can specifically amplify a parainfluenza virus type 3 L gene, the fourth group of primers and probes can specifically amplify a parainfluenza virus type 4 F gene, different groups of primers and probes do not interfere with each other, the typing effect on four serotypes of the parainfluenza virus is excellent, and the detection sensitivity and accuracy are high.
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Description

technical field

[0001] The invention belongs to the technical field of biomedicine, and in particular relates to a primer probe composition and a real-time fluorescent quantitative PCR kit for typing and detecting parainfluenza virus. Background technique

[0002] Parainfluenza virus is a kind of enveloped single-stranded negative-strand RNA virus belonging to the genus Paramyxovirus. It is a common pathogen of respiratory tract infection. It mainly causes severe lower respiratory tract infection in infants and children. At present, there is no effective treatment. Drugs and virus vaccines.

[0003] According to heredity and antigenicity, parainfluenza virus can be divided into type 1, type 2, type 3 and type 4. Type 1-3 parainfluenza virus is an important pathogen that causes acute respiratory infection in infants and young children, and type 4 only Causes respiratory infections in infants with mild symptoms. The symptoms of infection caused by parainfluenza virus are sim...

Claims

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