Fluorescent probe for simultaneously detecting mitochondrial membrane potential and quality through flow cytometer and synthesis method of fluorescent probe

Abstract

The invention relates to a fluorescent probe for simultaneously detecting mitochondrial membrane potential and quality through a flow cytometer and a synthesis method of the fluorescent probe. Aiming at the conditions that an existing fluorescent probe needs to occupy two fluorescent channels and is easily isolated from mitochondria when detecting the mitochondrial membrane potential in cells, when the probe is used on a flow cytometer to detect the mitochondrial membrane potential, only the excitation wavelength range of the same section is represented, and detection is carried out in the same fluorescent channel; and the probe can detect the quality of mitochondria while detecting the membrane potential of the mitochondria, so that the effect of jointly evaluating the mitochondria is achieved.

Description

technical field [0001] The invention relates to the field of fluorescent probe detection, in particular to a fluorescent probe for simultaneously detecting mitochondrial membrane potential and quality through a flow cytometer and a synthesis method thereof. Background technique [0002] Mitochondria are semi-autonomous organelles surrounded by double-layered highly specialized unit membranes in eukaryotic cells, which can generate energy to maintain normal physiological activities of cells. A large number of studies have shown that mitochondria participate in the growth, development, metabolism, Aging, disease, death, and biological evolution. Under different pathogenic stimuli, mitochondria are very prone to structural and functional damage, which directly affects the normal functions of other cell tissues in the body. When mitochondria are damaged, the membrane potential of mitochondria decreases, and the morphology and function change. In response to this damage, cells s...

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Problems solved by technology

However, the probe needs to occupy two fluorescent channels when used on a flow cytometer, which is not conducive to the simultaneous measurement of multiple indicators. At the same time, the fluorescent probe is easily isolated from the mitochondria. When the membrane potential of the mitochondria of the cell disappears, they are very easily washed off

This characteristic limits their application in some experiments, such as some cells need to be fixed by aldehydes or other reagents will affect the energy status of mitochondria, and traditional mitochondrial dyes cannot be used

There are also probes that are not dependent on mitochondrial membrane potential on the market, which can stain mitochondria in living cells and can also be retained in cells that have undergone a fixed process. Typical probes such as Mito Tracker Green can be used to detect mitochondrial quality. This indicator can reflect the morphological integrity of mitochondria, but the probe cannot reflect changes in the depolarization of mitochondrial membrane potential. At the same time, when detecting on a flow cytometer, the relationship between the fluorescence value detected by this probe and the quality of mitochondria is not clear, for example Different probe concentrations, different instrument states, etc. will affect the fluorescence value of the probe on the instrument

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