Myristoylated polypeptide for coding mitochondrial localization as well as preparation method and application of myristoylated polypeptide

A technology of myristoylation and mitochondria, which is applied in the field of molecular genetics, can solve the problems that foreign proteins cannot be imported into mitochondria, and achieve the effect of promoting combination
CN113307886AInactive Publication Date: 2021-08-27SUZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SUZHOU UNIV
Publication Date
2021-08-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a myristoylated polypeptide for coding mitochondrial localization as well as a preparation method and application thereof, and belongs to the technical field of molecular genetics. The polypeptide sequence has an amino acid sequence represented by an M-N formula, M is an amino acid sequence represented by SEQ ID No. 1, and N is an amino acid sequence represented by SEQ ID No. 2. The invention also discloses application of the polypeptide, a polypeptide composition, a nucleic acid and a polypeptide conjugate in expression of the myristoylated mitochondrial localization protein. The amino terminal of the sequence provided by the invention has a myristoylated conserved sequence which can be myristoylated and then inserted into a mitochondrial cell membrane.
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Description

technical field

[0001] The invention relates to the technical field of molecular genetics, in particular to a myristoylated polypeptide encoding mitochondrial location and its preparation method and application. Background technique

[0002] The present invention belongs to the field of molecular genetics. Mitochondrial function studies rely on mitochondrial co-localized marker genes and associated fluorescent dyes. However, how to artificially introduce fatty acid-modified target proteins into mitochondria is a difficult point in current research.

[0003] Observing and studying mitochondrial function relies on mitochondrial localized marker genes and fluorescent dyes. At the same time, by fusing the target gene with the mitochondrial guide peptide, the target protein encoded by the target gene can be introduced into the mitochondria. These methods make studying mitochondrial function simple and straightforward.

[0004] The introduction of fatty acid-modified target pr...

Claims

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